{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:24:56Z","timestamp":1793021096744,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T00:00:00Z","timestamp":1646611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cFondo de Investigaci\u00f3n Sanitaria, Instituto de Salud Carlos III, Ministerio de Econom\u00eda y Competitividad\u201d, Spain, cofunded by European Regional Development Fund of the European Union: a way to making Europe.","award":["FIS PI17\/00474"],"award-info":[{"award-number":["FIS PI17\/00474"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Non-typhoid serovars of Salmonella enterica are one of the main causes of bacterial food-borne infections worldwide. For the treatment of severe cases of salmonellosis in adults, fluoroquinolones are amongst the drugs of choice. They are categorized by the World Health Organization (WHO) as \u201ccritically important with highest priority in human medicine\u201d. In the present study, two clinical S. enterica serovar Corvallis isolates (HUA 5\/18 and HUA 6\/18) from a Spanish hospital, selected on the basis of fluoroquinolone resistance, were characterized. The MICs of ciprofloxacin, determined by E-test, were 0.5 and 0.75 \u00b5g\/mL for HUA 5\/18 and HUA 6\/18, respectively, and both were also resistant to pefloxacin but susceptible to nalidixic acid. Whole genome sequencing (WGS) of the isolates was performed with Illumina platform, and different bioinformatics tools were used for sequence analysis. The two isolates belonged to ST1541, and had the Thr57Ser substitution in the ParC protein which is also found in ciprofloxacin susceptible isolates. However, they harbored identical ColE plasmids of 10 kb carrying the qnrS1 gene. In these plasmids, the gene was flanked by defective versions of IS2-like and ISKra4-like insertion sequences. HUA 5\/18 and HUA 6\/18 were also phenotypically resistant to streptomycin, sulfonamides and tetracycline, with the responsible genes: strA, strB, sul2 and tet(A) genes, being located on a IncQ1 plasmid. ColE plasmids with the qnrS1 gene are widely spread among multiple serovars of S. enterica from different samples and countries. These mobilizable plasmids are playing an important role in the worldwide spread of qnrS1. Thus, their detection in hospitals is a cause of concern which deserves further attention.<\/jats:p>","DOI":"10.3390\/microorganisms10030579","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T01:53:54Z","timestamp":1646790834000},"page":"579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Plasmid-Mediated Quinolone Resistance (PMQR) in Two Clinical Strains of Salmonella enterica Serovar Corvallis"],"prefix":"10.3390","volume":"10","author":[{"given":"Xenia","family":"V\u00e1zquez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Universidad de Oviedo (UO), 33006 Oviedo, Spain"},{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7081-0936","authenticated-orcid":false,"given":"Javier","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain"},{"name":"Research & Innovation, Artificial Intelligence and Statistical Department, Pragmatech AI Solutions, 33003 Oviedo, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red-Enfermedades Respiratorias, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Hern\u00e1ez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario de \u00c1lava (HUA), 01009 \u00c1lava, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaura","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"},{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de Oviedo (UO), 33003 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3402-2908","authenticated-orcid":false,"given":"Maria Rosario","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Universidad de Oviedo (UO), 33006 Oviedo, Spain"},{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority and European Centre for Disease Prevention and Control (2021). 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