{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T03:39:32Z","timestamp":1769053172894,"version":"3.49.0"},"reference-count":69,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T00:00:00Z","timestamp":1647734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Aquatic environments are recognized as one of the main reservoirs for the emergence and dissemination of high-risk lineages of multidrug-resistant (MDR) bacteria of public health concern. However, the genomic characteristics of antibiotic-resistant Escherichia coli isolates from aquatic origins remain limited. Herein, we examined the antibiotic resistance and virulence genomic profiles of three E. coli recovered from surface water in northwest Mexico. Antimicrobial susceptibility testing, whole-genome sequencing (WGS), and in-depth in silico analysis were performed. Two E. coli exhibited MDR phenotypes. WGS-based typing revealed genetic diversity, and phylogenetic analysis corroborated a notable divergent relationship among the studied E. coli. One E. coli strain, harboring enterotoxigenic and extraintestinal pathogenic-associated virulence genes, was assigned to the ST4 lineage. MDR E. coli, belonging to the international high-risk clones ST410 and ST617, carried genes and mutations conferring resistance to aminoglycosides, \u03b2-lactams, quinolones, sulfonamides, tetracyclines, and trimethoprim. This study describes, for the first time, the detection and genomic profiling of high-risk lineages of E. coli ST410 and ST617 from surface water in Mexico. Additionally, our results underscore the role of surface water as a reservoir for critical pathogenic and MDR E. coli clones and the need for the surveillance and monitoring of aquatic environments via WGS from the One Health perspective.<\/jats:p>","DOI":"10.3390\/microorganisms10030662","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:34:27Z","timestamp":1647812067000},"page":"662","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genomic Profiling of Antibiotic-Resistant Escherichia coli Isolates from Surface Water of Agricultural Drainage in North-Western Mexico: Detection of the International High-Risk Lineages ST410 and ST617"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1534-6832","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"Maga\u00f1a-Liz\u00e1rraga","sequence":"first","affiliation":[{"name":"Unidad de Investigaciones en Salud P\u00fablica \u201cDra. Kaethe Willms\u201d, Facultad de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad Aut\u00f3noma de Sinaloa, Ciudad Universitaria, Culiacan 80013, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3695-3597","authenticated-orcid":false,"given":"Bruno","family":"G\u00f3mez-Gil","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo, A. C. (CIAD), Unidad Mazatl\u00e1n en Acuicultura y Manejo Ambiental, AP.711, Mazatlan 82112, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Guadalupe","family":"Rend\u00f3n-Maldonado","sequence":"additional","affiliation":[{"name":"Unidad de Investigaciones en Salud P\u00fablica \u201cDra. Kaethe Willms\u201d, Facultad de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad Aut\u00f3noma de Sinaloa, Ciudad Universitaria, Culiacan 80013, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3369-5200","authenticated-orcid":false,"given":"Francisco","family":"Delgado-Vargas","sequence":"additional","affiliation":[{"name":"Unidad de Investigaciones en Salud P\u00fablica \u201cDra. Kaethe Willms\u201d, Facultad de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad Aut\u00f3noma de Sinaloa, Ciudad Universitaria, Culiacan 80013, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In\u00e9s Fernando","family":"Vega-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Parque de Innovaci\u00f3n Tecnol\u00f3gica, Universidad Aut\u00f3noma de Sinaloa, Culiacan 80013, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Elena","family":"B\u00e1ez-Flores","sequence":"additional","affiliation":[{"name":"Unidad de Investigaciones en Salud P\u00fablica \u201cDra. Kaethe Willms\u201d, Facultad de Ciencias Qu\u00edmico Biol\u00f3gicas, Universidad Aut\u00f3noma de Sinaloa, Ciudad Universitaria, Culiacan 80013, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,20]]},"reference":[{"key":"ref_1","unstructured":"O\u2019Neill, J. (2016). 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