{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:46:51Z","timestamp":1780512411031,"version":"3.54.1"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["217173\/Z\/19\/Z"],"award-info":[{"award-number":["217173\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution, protein composition and structure. Apicomplexan mitochondrial translation and the mitoribosomes are essential in all parasites and life stages studied, highlighting mitoribosomes as a promising target for drugs. Still, the apicomplexan mitoribosome is understudied, with one of the obstacles being that its composition is unknown. Here, to facilitate the study of apicomplexan mitoribosomes, we identified and validated components of the mitoribosomal large subunit in the model apicomplexan Toxoplasma gondii.<\/jats:p>","DOI":"10.3390\/microorganisms10050863","type":"journal-article","created":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T21:39:45Z","timestamp":1650749985000},"page":"863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification and Validation of Toxoplasma gondii Mitoribosomal Large Subunit Components"],"prefix":"10.3390","volume":"10","author":[{"given":"Shikha","family":"Shikha","sequence":"first","affiliation":[{"name":"Wellcome Centre for Integrative Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Ferreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Wellcome Centre for Integrative Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5909-2307","authenticated-orcid":false,"given":"Lilach","family":"Sheiner","sequence":"additional","affiliation":[{"name":"Wellcome Centre for Integrative Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7235","DOI":"10.1074\/jbc.RA120.012646","article-title":"Genetic Ablation of the Mitoribosome in the Malaria Parasite Plasmodium Falciparum Sensitizes It to Antimalarials That Target Mitochondrial Functions","volume":"295","author":"Ling","year":"2020","journal-title":"J. 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