{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T17:24:42Z","timestamp":1769880282320,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Research Project of Key Laboratory of Guangzhou","award":["202102100001"],"award-info":[{"award-number":["202102100001"]}]},{"name":"Key Project of Agricultural and Social Development Science and Technology Projects of Guang-zhou","award":["202103000008"],"award-info":[{"award-number":["202103000008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Leptospirosis is a neglected zoonotic disease with global importance caused by pathogenic Leptospira. Rodents are considered the most significant reservoirs for both human and animal infection. Historically, Guangzhou has been an endemic region of human leptospirosis. Although the incidence in humans has significantly decreased in the past decades in China, the epidemiology of pathogenic Leptospira in wild rodents is of great significance for the prevention and control of human leptospirosis. In this study, a total of 296 wild rodents were trapped in urban areas of Guangzhou, in southern China, in 2020. Three pathogenic Leptospira species, i.e., Leptospira interrogans, L. borgpetersenii, and L. kirschneri, were detected by nested PCR in this wild rodent population with an overall prevalence of 9.5%. Additionally, L. interrogans was detected in three of the four captured rodent species, and the relative high prevalence suggests that L. interrogans probably represents the preponderant species of the pathogenic Leptospira circulating in Guangzhou. Taken together, this study reveals a high genetic diversity of pathogenic Leptospira disseminated among wild rodents in the urban areas of Guangzhou and emphasizes that the risk for the occurrence of human leptospirosis in Guangzhou remains high.<\/jats:p>","DOI":"10.3390\/microorganisms10050873","type":"journal-article","created":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T21:39:45Z","timestamp":1650749985000},"page":"873","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Pathogenic Leptospira Species Are Widely Disseminated among Wild Rodents in Urban Areas of Guangzhou, Southern China"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9548-3831","authenticated-orcid":false,"given":"Jian-Wei","family":"Shao","sequence":"first","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Hong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Parasitic Disease and Endemic Disease Control and Prevention, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Yan","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Parasitic Disease and Endemic Disease Control and Prevention, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7857-6507","authenticated-orcid":false,"given":"Shou-Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Parasitic Disease and Endemic Disease Control and Prevention, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China"},{"name":"Institute of Public Health, Guangzhou Medical University, Guangzhou 511436, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: A zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., and Ko, A.I. (2015). Global morbidity and mortality of Leptospirosis: A systematic review. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003898"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1111\/j.1469-0691.2011.03474.x","article-title":"Emergence, control and re-emerging leptospirosis: Dynamics of infection in the changing world","volume":"17","author":"Hartskeerl","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1097\/QCO.0000000000000001","article-title":"Current trends in translational research in leptospirosis","volume":"26","author":"Ricaldi","year":"2013","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Biggs, H.M., Hertz, J.T., Munishi, O.M., Galloway, R.L., Marks, F., Saganda, W., Maro, V.P., and Crump, J.A. (2013). Estimating leptospirosis incidence using hospital-based surveillance and a population-based health care utilization survey in Tanzania. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002589"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cimid.2013.12.002","article-title":"Human leptospirosis: An emerging risk in Europe?","volume":"37","author":"Dupouey","year":"2014","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.jiph.2017.07.024","article-title":"Human leptospirosis cases in Palermo Italy. The role of rodents and climate","volume":"11","author":"Vitale","year":"2018","journal-title":"J. Infect. Public Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nrmicro.2017.5","article-title":"Virulence of the zoonotic agent of leptospirosis: Still terra incognita?","volume":"15","author":"Picardeau","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Thaipadungpanit, J., Wuthiekanun, V., Chierakul, W., Smythe, L.D., Petkanchanapong, W., Limpaiboon, R., Apiwatanaporn, A., Slack, A.T., Suputtamongkol, Y., and White, N.J. (2007). A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Negl. Trop. Dis., 1.","DOI":"10.1371\/journal.pntd.0000056"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1097\/QCO.0000000000000097","article-title":"Leptospira and leptospirosis in China","volume":"27","author":"Hu","year":"2014","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.micinf.2011.11.007","article-title":"Leptospirosis prevalence in Chinese populations in the last two decades","volume":"14","author":"Zhang","year":"2012","journal-title":"Microbes Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s40249-018-0435-2","article-title":"Epidemiological shift and geographical heterogeneity in the burden of leptospirosis in China","volume":"7","author":"Dhewantara","year":"2018","journal-title":"Infect. Dis. Poverty"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1111\/tbed.13437","article-title":"Leptospirosis trends in China, 2007\u20132018: A retrospective observational study","volume":"67","author":"Zhang","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_15","first-page":"99","article-title":"Animal leptospirosis","volume":"387","author":"Ellis","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1089\/vbz.2019.2544","article-title":"Detection of Leptospira interrogans in Hedgehogs from central China","volume":"20","author":"Ma","year":"2020","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/10408410902989837","article-title":"Rodent-borne diseases and their risks for public health","volume":"35","author":"Meerburg","year":"2009","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Guo, W.P., Lin, X.D., Wang, W., Tian, J.H., Cong, M.L., Zhang, H.L., Wang, M.R., Zhou, R.H., Wang, J.B., and Li, M.H. (2013). Phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodents. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003159"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.diagmicrobio.2008.10.017","article-title":"A simple and rapid nested polymerase chain reaction-restriction fragment length polymorphism technique for differentiation of pathogenic and nonpathogenic Leptospira spp.","volume":"63","author":"Djadid","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Bourhy, P., Herrmann Storck, C., Theodose, R., Olive, C., Nicolas, M., Hochedez, P., Lamaury, I., Zinini, F., Br\u00e9mont, S., and Landier, A. (2013). Serovar diversity of pathogenic Leptospira circulating in the French West Indies. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002114"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Guernier, V., Richard, V., Nhan, T., Rouault, E., Tessier, A., and Musso, D. (2017). Leptospira diversity in animals and humans in Tahiti, French Polynesia. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005676"},{"key":"ref_22","first-page":"71","article-title":"DNASTAR\u2019s Lasergene sequence analysis software","volume":"132","author":"Burland","year":"2000","journal-title":"Methods Mol. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1093\/molbev\/msn083","article-title":"jModelTest: Phylogenetic model averaging","volume":"25","author":"Posada","year":"2008","journal-title":"Mol. Biol. Evol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4564","DOI":"10.1021\/pr9004597","article-title":"Global proteome analysis of Leptospira interrogans","volume":"8","author":"Eshghi","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"14560","DOI":"10.1073\/pnas.0603979103","article-title":"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential","volume":"103","author":"Bulach","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhang, C., Xu, J., Zhang, T., Qiu, H., Li, Z., Zhang, E., Li, S., Chang, Y.F., Guo, X., and Jiang, X. (2019). Genetic characteristics of pathogenic Leptospira in wild small animals and livestock in Jiangxi Province, China, 2002-2015. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007513"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/zph.12717","article-title":"Epidemiology and genetic diversity of zoonotic pathogens in urban rats (Rattus spp.) from a subtropical city, Guangzhou, southern China","volume":"67","author":"Su","year":"2020","journal-title":"Zoonoses Public Health"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhang, C., Yang, H., Li, X., Cao, Z., Zhou, H., Zeng, L., Xu, J., Xu, Y., Chang, Y.F., and Guo, X. (2015). Molecular typing of pathogenic Leptospira serogroup icterohaemorrhagiae strains circulating in China during the past 50 years. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003762"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1073\/pnas.95.6.3140","article-title":"Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms","volume":"95","author":"Maiden","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jcpa.2007.08.001","article-title":"Morphological alterations in the kidney of rats with natural and experimental Leptospira infection","volume":"137","author":"Athanazio","year":"2007","journal-title":"J. Comp. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.actatropica.2019.02.008","article-title":"Molecular epidemiology of Leptospira interrogans in Rattus norvegicus in Hanoi, Vietnam","volume":"194","author":"Koizumi","year":"2019","journal-title":"Acta Trop."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.actatropica.2017.07.032","article-title":"Renal carriage of Leptospira species in rodents from Mediterranean Chile: The Norway rat (Rattus norvegicus) as a relevant host in agricultural lands","volume":"176","author":"Correa","year":"2017","journal-title":"Acta Trop."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Boey, K., Shiokawa, K., and Rajeev, S. (2019). Leptospira infection in rats: A literature review of global prevalence and distribution. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007499"},{"key":"ref_35","first-page":"161","article-title":"Isolation by culture and PCR identification of LipL32 gene of pathogenic Leptospira spp. in wild rats of Kuala Lumpur","volume":"39","author":"Latifah","year":"2017","journal-title":"Malays. J. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Himsworth, C.G., Bidulka, J., Parsons, K.L., Feng, A.Y.T., Tang, P., Jardine, C.M., Kerr, T., Mak, S., Robinson, J., and Patrick, D.M. (2013). Ecology of Leptospira interrogans in Norway rats (Rattus norvegicus) in an inner-city neighborhood of Vancouver, Canada. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002270"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1089\/vbz.2012.1195","article-title":"Rats, cities, people, and pathogens: A systematic review and narrative synthesis of literature regarding the ecology of rat-associated zoonoses in urban centers","volume":"13","author":"Himsworth","year":"2013","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Minter, A., Himsworth, C.G., Byers, K.A., Childs, J.E., Ko, A.I., and Costa, F. (2019). Tails of two cities: Age and wounding are associated with carriage of Leptospira interrogans by Norway rats (Rattus norvegicus) in ecologically distinct urban environments. Front. Ecol. Evol., 7.","DOI":"10.3389\/fevo.2019.00014"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Wei, Y., Wang, Y., Li, X., Qin, P., Lu, Y., Xu, J., Chen, S., Li, M., and Yang, Z. (2018). Meteorological factors and risk of hemorrhagic fever with renal syndrome in Guangzhou, southern China, 2006\u20132015. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006604"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/5\/873\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:58:38Z","timestamp":1760137118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/5\/873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,22]]},"references-count":39,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["microorganisms10050873"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10050873","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,22]]}}}