{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:16:31Z","timestamp":1790226991012,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T00:00:00Z","timestamp":1651881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Biofertilizers are a key component of organic agriculture. Bacterial biofertilizers enhance plant growth through a variety of mechanisms, including soil compound mobilization and phosphate solubilizing bacteria (PSB), which convert insoluble phosphorus to plant-available forms. This specificity of PSB allows them to be used as biofertilizers in order to increase P availability, which is an immobile element in the soil. The objective of our study is to assess the capacity of PSB strains isolated from phosphate solid sludge to solubilize three forms of inorganic phosphates: tricalcium phosphate (Ca3(PO4)2), aluminum phosphate (AlPO4), and iron phosphate (FePO4), in order to select efficient solubilization strains and use them as biofertilizers in any type of soil, either acidic or calcareous soil. Nine strains were selected and they were evaluated for their ability to dissolve phosphate in the National Botanical Research Institute\u2019s Phosphate (NBRIP) medium with each form of phosphate (Ca3(PO4)2, AlPO4, and FePO4) as the sole source of phosphorus. The phosphate solubilizing activity was assessed by the vanadate-molybdate method. All the strains tested showed significantly (p \u2264 0.05) the ability to solubilize the three different forms of phosphates, with a variation between strains, and all strains solubilized Ca3(PO4)2 more than FePO4 and AlPO4.<\/jats:p>","DOI":"10.3390\/microorganisms10050980","type":"journal-article","created":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T21:31:20Z","timestamp":1652045480000},"page":"980","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Phosphate-Solubilizing Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilize Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4288-8012","authenticated-orcid":false,"given":"Fatima Zahra","family":"Aliyat","sequence":"first","affiliation":[{"name":"Environment and Valorization of Microbial and Plant Resources Unit, Faculty of Sciences, Moulay Ismail University, Meknes 50070, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Maldani","sequence":"additional","affiliation":[{"name":"Department of Biological & Forensic Sciences, Fayetteville State University, 1200 Murchison Road, Fayetteville, NC 28301, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"El Guilli","sequence":"additional","affiliation":[{"name":"National Institute of Agricultural Research, Regional Center for Agricultural Research, Kenitra 14000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila","family":"Nassiri","sequence":"additional","affiliation":[{"name":"Environment and Valorization of Microbial and Plant Resources Unit, Faculty of Sciences, Moulay Ismail University, Meknes 50070, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamal","family":"Ibijbijen","sequence":"additional","affiliation":[{"name":"Environment and Valorization of Microbial and Plant Resources Unit, Faculty of Sciences, Moulay Ismail University, Meknes 50070, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.apsoil.2005.12.002","article-title":"Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities","volume":"34","author":"Chen","year":"2006","journal-title":"Appl. Soil Ecol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hegyi, A., Bao, T., Nguyen, K., and Posta, K. (2021). Metagenomic analysis of bacterial communities in agricultural soils from Vietnam with special attention to phosphate solubilizing bacteria. Microorganisms, 9.","DOI":"10.3390\/microorganisms9091796"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zhang, C., Cai, K., Li, M., Zheng, J., and Han, Y. (2022). Plant-growth-promoting potential of PGPE isolated from Dactylis glomerata L.. Microorganisms, 10.","DOI":"10.3390\/microorganisms10040731"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.32604\/phyton.2021.016174","article-title":"Pathways of phosphorus absorption and early signaling between the mycorrhizal fungi and plants","volume":"90","year":"2021","journal-title":"Phyton"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/BF00029076","article-title":"A new method for estimating gross phosphorus mineralization and immobilization rates in soils","volume":"147","author":"Zou","year":"1992","journal-title":"Plant Soil"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.apsoil.2016.11.010","article-title":"Dark septate endophytes present different potential to solubilize calcium, iron and aluminum phosphates","volume":"111","author":"Spagnoletti","year":"2016","journal-title":"Appl. Soil Ecol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Holtan, H., and Stuanes, A. (1985, January 25\u201328). Phosphorus in soil, water and sediment: An overview. Proceedings of the 1988 Phosphorus in Freshwater Ecosystems Symposium, Uppsala, Sweden.","DOI":"10.1007\/978-94-009-3109-1_3"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s00374-012-0737-7","article-title":"Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: A proposal for an alternative procedure","volume":"49","author":"Bashan","year":"2012","journal-title":"Biol. Fertil. Soils"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0734-9750(99)00014-2","article-title":"Phosphate solubilizing bacteria and their role in plant growth promotion","volume":"17","author":"Fraga","year":"1999","journal-title":"Biotechnol. Adv."},{"key":"ref_10","first-page":"16","article-title":"Isolation and characterization of phosphate solubilizing bacteria from phosphate solid sludge of the Moroccan phosphate mines","volume":"14","author":"Aliyat","year":"2020","journal-title":"Open Agric. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jgeb.2017.01.003","article-title":"Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi River delta, Odisha, India","volume":"15","author":"Behera","year":"2017","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1080\/01490451.2019.1709108","article-title":"Isolation and characterization of rhizospheric phosphate solubilizing bacteria from wetland paddy field of Mizoram, India","volume":"37","author":"Chawngthu","year":"2020","journal-title":"Geomicrobiol. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1007\/s00284-020-02080-6","article-title":"Variation in the phosphate solubilizing bacteria from virgin and the agricultural soils of Punjab","volume":"77","author":"Kaur","year":"2020","journal-title":"Curr. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1080\/01490451.2016.1146373","article-title":"Isolation of phosphate solubilizing bacteria from maize rhizosphere and their potential for rock phosphate solubilization\u2013mineralization and plant growth promotion","volume":"34","author":"Manzoor","year":"2017","journal-title":"Geomicrobiol. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1080\/01490451.2019.1694106","article-title":"Isolation, selection and characterization of root-associated rock phosphate solubilizing bacteria in Moroccan wheat (Triticum aestivum L.)","volume":"37","author":"Rfaki","year":"2020","journal-title":"Geomicrobiol. J."},{"key":"ref_16","unstructured":"Premono, M.E., Moawad, A.M., and Vlek, P.L.G. (1996). Effect of Phosphate-Solubilizing Pseudomonas Putida on the Growth of Maize and Its Survival in the Rhizosphere, Centro Internacional de Mejoramiento de Ma\u00edz y Trigo, (CIMMYT)."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.scitotenv.2018.08.318","article-title":"Impacts of Mo application on biological nitrogen fixation and diazotrophic communities in a flooded rice-soil system","volume":"649","author":"Ma","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/j.1574-6968.1999.tb13383.x","article-title":"An efficient microbiological growth medium for screening phosphate solubilizing microorganisms","volume":"170","author":"Nautiyal","year":"1999","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_20","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1104\/pp.26.1.192","article-title":"Colorimetric estimation of indoleacetic acid","volume":"26","author":"Gordon","year":"1951","journal-title":"Plant Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0003-2697(87)90612-9","article-title":"Universal chemical assay for the detection and determination of siderophores","volume":"160","author":"Schwyn","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0038-0717(87)90037-X","article-title":"Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas SPP-mediated plant growth-stimulation","volume":"19","author":"Bakker","year":"1987","journal-title":"Soil Biol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1007\/s11274-006-9144-2","article-title":"Two phosphate-and potassium-solubilizing bacteria isolated from Tianmu mountain, Zhejiang, China","volume":"22","author":"Hu","year":"2006","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1007\/s003740050024","article-title":"Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon","volume":"30","author":"Vazquez","year":"2000","journal-title":"Biol. Fertil. Soils"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1001\/jama.1907.25320140022001f","article-title":"The nephelometer: An instrument for media used for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines","volume":"49","author":"McFaland","year":"1907","journal-title":"J. Am. Med. Assoc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0003-2670(00)88444-5","article-title":"A modified single solution method for the determination of phosphate in natural waters","volume":"27","author":"Murphy","year":"1962","journal-title":"Anal. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0232-4393(83)80060-2","article-title":"Phosphate-solubilizing potentiality of the microorganisms capable of utilizing aluminium phosphate as a sole phosphate source","volume":"138","author":"Banik","year":"1983","journal-title":"Zent. Mikrobiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1080\/00380768.2002.10409246","article-title":"New isolation method for microorganisms solubilizing iron and aluminum phosphates using dyes","volume":"48","author":"Gadagi","year":"2002","journal-title":"Soil Sci. Plant Nutr."},{"key":"ref_30","first-page":"667","article-title":"Solubilization of different phosphate forms by phosphate solubilizing bacteria isolated from aerobic rice","volume":"11","author":"Panhwar","year":"2009","journal-title":"Int. J. Agric. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"306","DOI":"10.5958\/0974-0228.2021.00054.2","article-title":"Diversity of multifunctional phosphorus solubilizing bacteria in acid soils of diverse hill rice ecosystems","volume":"69","author":"Devi","year":"2021","journal-title":"J. Indian Soc. Soil Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1574-6941.1999.tb00584.x","article-title":"Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants","volume":"28","author":"Reyes","year":"1999","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_33","first-page":"439","article-title":"Solubilization of insoluble inorganic phosphates by a soil-inhabiting Fungus fomitopsis sp. PS 102","volume":"82","author":"Kang","year":"2002","journal-title":"Curr. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12223-022-00953-w","article-title":"Solubilization of rock phosphate by phosphate solubilizing bacteria isolated from effluent treatment plant sludge of a fertilizer plant","volume":"67","author":"Maharana","year":"2022","journal-title":"Folia Microbiol."},{"key":"ref_35","first-page":"1","article-title":"Selective enrichment method for isolation of efficient phosphate solubilizing bacteria from soil","volume":"53","author":"Patel","year":"2022","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100448","DOI":"10.1016\/j.rhisph.2021.100448","article-title":"Phosphorus solubilizing bacteria Bacillus thuringiensis and Pantoea ananatis simultaneously promote soil inorganic phosphate dissolution and soil Pb immobilization","volume":"20","author":"Liu","year":"2021","journal-title":"Rhizosphere"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Z\u00fa\u00f1iga-Silgado, D., Rivera-Leyva, J.C., Coleman, J.J., S\u00e1nchez-Reyez, A., Valencia-D\u00edaz, S., Serrano, M., De-Bashan, L.E., and Folch-Mallol, J.L. (2020). Soil type affects organic acid production and phosphorus solubilization efficiency mediated by several native fungal strains from Mexico. Microorganisms, 8.","DOI":"10.3390\/microorganisms8091337"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ma\u017eylyt\u0117, R., Kazi\u016bnien\u0117, J., Orola, L., Valkovska, V., Lastauskien\u0117, E., and Gegeckas, A. (2022). Phosphate solubilizing microorganism Bacillus sp. MVY-004 and its significance for biomineral fertilizers\u2019 development in agrobiotechnology. Biology, 11.","DOI":"10.3390\/biology11020254"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00572-021-01067-w","article-title":"Desert truffle mycorrhizosphere harbors organic acid releasing plant growth\u2013promoting rhizobacteria, essentially during the truffle fruiting season","volume":"32","author":"Arenas","year":"2022","journal-title":"Mycorrhiza"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1007\/s42729-021-00709-4","article-title":"Optimization of culture conditions for zinc phosphate solubilization by Aspergillus sp. using response surface methodology","volume":"22","author":"Barin","year":"2022","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1016\/j.scitotenv.2018.06.399","article-title":"Bacteria from nodules of wild legume species: Phylogenetic diversity, plant growth promotion abilities and osmotolerance","volume":"645","author":"Cardoso","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1007\/BF00327716","article-title":"Factors determining rock phosphate solubilization by microorganisms isolated from soil","volume":"12","author":"Nahas","year":"1996","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_43","first-page":"37","article-title":"Phosphate solubilizing microbes: An effective and alternative approach as biofertilizers","volume":"8","author":"Anand","year":"2016","journal-title":"Int. J. Pharm. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1023\/A:1008830129262","article-title":"The role of rock-phosphate-solubilizing fungi and vesicular\u2013arbusular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate","volume":"14","author":"Omar","year":"1997","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s12088-008-0037-y","article-title":"Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh","volume":"48","author":"Chatli","year":"2008","journal-title":"Indian J. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Vasseur-Coronado, M., Vlassi, A., du Boulois, H., Schuhmacher, R., Parich, A., Pertot, I., and Puopolo, G. (2021). Ecological role of volatile organic compounds emitted by Pantoea agglomerans as interspecies and interkingdom signals. Microorganisms, 9.","DOI":"10.3390\/microorganisms9061186"},{"key":"ref_47","first-page":"174","article-title":"Phosphorus availability issue\u2014Its fixation and role of phosphate solubilizing bacteria in phosphate solubilization\u2014Case study","volume":"2","author":"Mahidi","year":"2011","journal-title":"Agric. Sci. Res. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"220605","DOI":"10.13057\/biodiv\/d220605","article-title":"Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer","volume":"22","author":"Elfiati","year":"2021","journal-title":"Biodiversitas J. Biol. Divers."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0065-2113(01)74031-X","article-title":"Organic acids exuded from roots in phosphorus uptake and aluminum tolerance of plants in acid soils","volume":"74","author":"Hocking","year":"2001","journal-title":"Adv. Agron."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.flora.2018.01.006","article-title":"Pb stress effects on leaf chlorophyll fluorescence, antioxidative enzyme activities, and organic acid contents of Pogonatherum crinitum seedlings","volume":"240","author":"Hou","year":"2018","journal-title":"Flora Morphol. Distrib. Funct. Ecol. Plants"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s42729-020-00342-7","article-title":"Phosphate-solubilizing microorganisms: Mechanism and their role in phosphate solubilization and uptake","volume":"21","author":"Rawat","year":"2020","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_52","first-page":"108","article-title":"Effect of co inoculation of potassium solubilizing, mobilizing and phosphorus solubilizing bacteria on growth, yield and nutrient uptake of radish (Raphanus sativus L)","volume":"8","author":"Biswas","year":"2021","journal-title":"Int. J. Adv. Res. Biol. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1007\/s00284-008-9327-1","article-title":"Characterization of the mineral phosphate-solubilizing activity of Pantoea aglomerans MMB051 isolated from an iron-rich soil in southeastern Venezuela (Bol\u00edvar state)","volume":"58","author":"Ball","year":"2009","journal-title":"Curr. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Benjelloun, I., Thami Alami, I., El Khadir, M., Douira, A., and Udupa, S.M. (2021). Co-inoculation of Mesorhizobium ciceri with either Bacillus sp. or Enterobacter aerogenes on chickpea improves growth and productivity in phosphate-deficient soils in dry areas of a Mediterranean region. Plants, 10.","DOI":"10.3390\/plants10030571"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s00248-021-01702-1","article-title":"Plant growth promotion at low temperature by phosphate-solubilizing Pseudomonas spp. isolated from high-altitude Himalayan soil","volume":"82","author":"Adhikari","year":"2021","journal-title":"Microb. Ecol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s13580-018-00121-5","article-title":"Endogenous hormone levels and activities of IAA-modifying enzymes during adventitious rooting of tree peony cuttings and grafted scions","volume":"60","author":"Meng","year":"2019","journal-title":"Hortic. Environ. Biotechnol."},{"key":"ref_57","first-page":"100150","article-title":"Screening of siderophore-producing salt-tolerant rhizobacteria suitable for supporting plant growth in saline soils with iron limitation","volume":"4","author":"Sultana","year":"2021","journal-title":"J. Agric. Food Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.tibtech.2009.12.002","article-title":"Potential of siderophore-producing bacteria for improving heavy metal phytoextraction","volume":"28","author":"Rajkumar","year":"2010","journal-title":"Trends Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1007\/s00203-021-02284-9","article-title":"Isolation and screening of potassium solubilizing bacteria from saxicolous habitat and their impact on tomato growth in different soil types","volume":"203","author":"Raji","year":"2021","journal-title":"Arch. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/5\/980\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:07:44Z","timestamp":1760137664000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/5\/980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,7]]},"references-count":59,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["microorganisms10050980"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10050980","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,7]]}}}