{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:35:54Z","timestamp":1787063754852,"version":"build-2736575974"},"reference-count":85,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Council for Science and Technological Development (CNPq)","award":["311283\/2020-9"],"award-info":[{"award-number":["311283\/2020-9"]}]},{"name":"National Council for Science and Technological Development (CNPq)","award":["2017\/14821-7"],"award-info":[{"award-number":["2017\/14821-7"]}]},{"name":"National Council for Science and Technological Development (CNPq)","award":["2017\/21947-7"],"award-info":[{"award-number":["2017\/21947-7"]}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","doi-asserted-by":"publisher","award":["311283\/2020-9"],"award-info":[{"award-number":["311283\/2020-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","doi-asserted-by":"publisher","award":["2017\/14821-7"],"award-info":[{"award-number":["2017\/14821-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","doi-asserted-by":"publisher","award":["2017\/21947-7"],"award-info":[{"award-number":["2017\/21947-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>While primarily Gram-positive bacteria cause bacterial eye infections, several Gram-negative species also pose eye health risks. Currently, few studies have tried to understand the pathogenic mechanisms involved in E. coli eye infections. Therefore, this study aimed to establish the pathogenic potential of E. coli strains isolated from eye infections. Twenty-two strains isolated between 2005 and 2019 from patients with keratitis or conjunctivitis were included and submitted to traditional polymerase chain reactions (PCR) to define their virulence profile, phylogeny, clonal relationship, and sequence type (ST). Phenotypic assays were employed to determine hemolytic activity, antimicrobial susceptibility, and adhesion to human primary corneal epithelial cells (PCS-700-010). The phylogenetic results indicated that groups B2 and ST131 were the most frequent. Twenty-five virulence genes were found among our strains, with ecp, sitA, fimA, and fyuA being the most prevalent. Two strains presented a hemolytic phenotype, and resistance to ciprofloxacin and ertapenem was found in six strains and one strain, respectively. Regarding adherence, all but one strains adhered in vitro to corneal cells. Our results indicate significant genetic and virulence variation among ocular strains and point to an ocular pathogenic potential related to multiple virulence mechanisms.<\/jats:p>","DOI":"10.3390\/microorganisms10061084","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T05:12:27Z","timestamp":1653455547000},"page":"1084","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of the Pathogenic Potential of Escherichia coli Strains Isolated from Eye Infections"],"prefix":"10.3390","volume":"10","author":[{"given":"Pedro Henrique Soares","family":"Nunes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Experimental de Patogenicidade de Enterobact\u00e9rias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"},{"name":"Laborat\u00f3rio de Oftalmologia (LOFT), Departamento de Oftalmologia e Ci\u00eancias Visuais, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Barcelos","family":"Valiatti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Experimental de Patogenicidade de Enterobact\u00e9rias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"},{"name":"Laborat\u00f3rio Alerta, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04039-032, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0833-4585","authenticated-orcid":false,"given":"Ana Carolina de Mello","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Experimental de Patogenicidade de Enterobact\u00e9rias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7054-0817","authenticated-orcid":false,"given":"J\u00fallia Assis da Silva","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Experimental de Patogenicidade de Enterobact\u00e9rias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6407-5947","authenticated-orcid":false,"given":"Jos\u00e9 Francisco","family":"Santos-Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Experimental de Patogenicidade de Enterobact\u00e9rias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita Trevizani","family":"Rocchetti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Oftalmologia (LOFT), Departamento de Oftalmologia e Ci\u00eancias Visuais, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cecilia Zorat","family":"Yu","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Oftalmologia (LOFT), Departamento de Oftalmologia e Ci\u00eancias Visuais, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luisa","family":"Hofling-Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Oftalmologia (LOFT), Departamento de Oftalmologia e Ci\u00eancias Visuais, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4525-8705","authenticated-orcid":false,"given":"T\u00e2nia Aparecida Tardelli","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Experimental de Patogenicidade de Enterobact\u00e9rias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.exer.2013.06.003","article-title":"Characterization of the normal microbiota of the ocular surface","volume":"117","author":"Willcox","year":"2013","journal-title":"Exp. 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