{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:09:15Z","timestamp":1791047355639,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"RUSSIAN SCIENCE FOUNDATION","doi-asserted-by":"publisher","award":["20-14-00137"],"award-info":[{"award-number":["20-14-00137"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The metagenome of foulings from sulfidic spring \u201cSerovodorodny\u201d (Tatarstan, Russia), where members of the genus Thiothrix was observed, was sequenced. Representatives of the phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in the microbial community. The complete genome of Thiothrix sp. KT was assembled from the metagenome. It displayed 93.93\u201399.72% 16S rRNA gene sequence identity to other Thiothrix species. The average nucleotide identity (ANI) \u0438 digital DNA-DNA hybridization (dDDH) showed that the genome designated KT represents a new species within the genus Thiothrix, \u2018Candidatus Thiothrix sulfatifontis\u2019 sp. nov. KT. The taxonomic status has been determined of the strain Thiothrix sp. CT3, isolated about 30 years ago and not assigned to any of Thiothrix species due to high 16S rRNA gene sequence identity with related species (i.e., 98.8\u201399.4%). The complete genome sequence of strain CT3 was determined. The ANI between CT3 and other Thiothrix species was below 82%, and the dDDH values were less than 40%, indicating that strain CT3 belongs to a novel species, Thiothrix winogradskyi sp. nov. A genome analysis showed that both strains are chemo-organoheterotrophs, chemolithotrophs (in the presence of hydrogen sulfide and thiosulfate) and chemoautotrophs. For the first time, representatives of Thiothrix showed anaerobic growth in the presence of thiosulfate.<\/jats:p>","DOI":"10.3390\/microorganisms10071300","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:07:02Z","timestamp":1656374822000},"page":"1300","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and \u2018Candidatus Thiothrix sulfatifontis\u2019 sp. nov."],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1456-1832","authenticated-orcid":false,"given":"Nikolai","family":"Ravin","sequence":"first","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9474-0496","authenticated-orcid":false,"given":"Simona","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Water Research Institute, IRSA-CNR, Monterotondo, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Beletsky","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaly","family":"Kadnikov","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-4115","authenticated-orcid":false,"given":"Tatyana","family":"Rudenko","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, 394018 Voronezh, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8500-5366","authenticated-orcid":false,"given":"Dmitry","family":"Smolyakov","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, 394018 Voronezh, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Moskvitina","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, 394018 Voronezh, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4887-794X","authenticated-orcid":false,"given":"Maria","family":"Gureeva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, 394018 Voronezh, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8245-8757","authenticated-orcid":false,"given":"Andrey","family":"Mardanov","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7877-3829","authenticated-orcid":false,"given":"Margarita","family":"Grabovich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, 394018 Voronezh, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"\u00a0Thiothrix","volume":"Volume 2","author":"Unz","year":"2005","journal-title":"Bergey\u2019s Manual of Systematic Bacteriology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1128\/aem.49.4.887-898.1985","article-title":"Filamentous sulfur bacteria of activated sludge: Characterization of Thiothrix, Beggiatoa, and Eikelboom type 021N strains","volume":"49","author":"Williams","year":"1985","journal-title":"Appl. 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