{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:51:56Z","timestamp":1790700716712,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French GIS IBiSA project dehaloDeepSeq"},{"name":"the French Ministry of Higher Education and Research"},{"name":"R\u00e9gion Alsace (France)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Dichloromethane (DCM, methylene chloride) is a toxic halogenated volatile organic compound massively used for industrial applications, and consequently often detected in the environment as a major pollutant. DCM biotransformation suggests a sustainable decontamination strategy of polluted sites. Among methylotrophic bacteria able to use DCM as a sole source of carbon and energy for growth, Methylorubrum extorquens DM4 is a longstanding reference strain. Here, the primary 5\u2032-ends of transcripts were obtained using a differential RNA-seq (dRNA-seq) approach to provide the first transcription start site (TSS) genome-wide landscape of a methylotroph using DCM or methanol. In total, 7231 putative TSSs were annotated and classified with respect to their localization to coding sequences (CDSs). TSSs on the opposite strand of CDS (antisense TSS) account for 31% of all identified TSSs. One-third of the detected TSSs were located at a distance to the start codon inferior to 250 nt (average of 84 nt) with 7% of leaderless mRNA. Taken together, the global TSS map for bacterial growth using DCM or methanol will facilitate future studies in which transcriptional regulation is crucial, and efficient DCM removal at polluted sites is limited by regulatory processes.<\/jats:p>","DOI":"10.3390\/microorganisms10071301","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:07:02Z","timestamp":1656374822000},"page":"1301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genome-Wide Transcription Start Sites Mapping in Methylorubrum Grown with Dichloromethane and Methanol"],"prefix":"10.3390","volume":"10","author":[{"given":"Bruno","family":"Maucourt","sequence":"first","affiliation":[{"name":"G\u00e9n\u00e9tique Mol\u00e9culaire, G\u00e9nomique, Microbiologie, Universit\u00e9 de Strasbourg, UMR 7156 CNRS, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2366-0311","authenticated-orcid":false,"given":"David","family":"Roche","sequence":"additional","affiliation":[{"name":"LABGeM, G\u00e9nomique M\u00e9tabolique, G\u00e9noscope, Institut de Biologie Fran\u00e7ois Jacob, Commissariat \u00e0 l\u2019Energie Atomique (CEA), CNRS, Universit\u00e9 Evry, Universit\u00e9 Paris-Saclay, 91057 Evry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Chaignaud","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9tique Mol\u00e9culaire, G\u00e9nomique, Microbiologie, Universit\u00e9 de Strasbourg, UMR 7156 CNRS, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2232-7023","authenticated-orcid":false,"given":"St\u00e9phane","family":"Vuilleumier","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9tique Mol\u00e9culaire, G\u00e9nomique, Microbiologie, Universit\u00e9 de Strasbourg, UMR 7156 CNRS, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4529-5143","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Bringel","sequence":"additional","affiliation":[{"name":"G\u00e9n\u00e9tique Mol\u00e9culaire, G\u00e9nomique, Microbiologie, Universit\u00e9 de Strasbourg, UMR 7156 CNRS, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1002\/jat.3979","article-title":"Solvent-based paint and varnish removers: A focused toxicologic review of existing and alternative constituents","volume":"40","author":"Durrani","year":"2020","journal-title":"J. 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