{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:09:26Z","timestamp":1790726966893,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cPHC Utique\u201d program of the French Ministry of Foreign Affairs, the French Ministry of Higher Education, Research and Innovation and the Tunisian Ministry of Higher Education and Scientific Research","award":["21G0807"],"award-info":[{"award-number":["21G0807"]}]},{"name":"internal funding from the French Agency for Food, Environmental and Occupational Health &amp; Safety (ANSES)","award":["21G0807"],"award-info":[{"award-number":["21G0807"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Aquaculture is a rapidly expanding sector in which it is important to monitor the occurrence of multi-drug resistant (MDR) bacteria. The presence of extended-spectrum \u03b2-lactamase (ESBL-) or carbapenemase-producing Enterobacterales is a commonly used indicator of the resistance burden in a given sector. In this study, 641 pieces of farmed fish (sea bream and sea bass), as well as 1075 Mediterranean clams, were analyzed. All ESBL- and carbapenemase-producing Enterobacterales collected were whole-genome sequenced. The proportion of ESBL-producing Enterobacterales was 1.4% in fish and 1.6% in clams, carried by Escherichia coli (n = 23) and Klebsiella pneumoniae (n = 4). The ESBL phenotype was exclusively due to the presence of blaCTX-M genes, the most frequent one being blaCTX-M-15. The blaCTX-M-1 gene was also identified in six E. coli, among which four were carried by IncI1\/pST3 plasmids, possibly betraying an animal origin. Carbapenemases were absent in fish but identified in two K. pneumoniae isolates from clams (blaNDM-1 and blaOXA-48). Several sequence types (STs) identified were associated with human MDR clones such as E. coli ST131 and ST617, or K. pneumoniae ST307 and ST147. Our results might indicate that bacteria from hospital or farm effluents can reach the open sea and contaminate seafood and fish that are living or raised nearby. Therefore, monitoring the quality of water discharged to the sea and the presence of MDR bacteria in seafood is mandatory to ensure the quality of fishery products.<\/jats:p>","DOI":"10.3390\/microorganisms10071364","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T09:41:10Z","timestamp":1657100470000},"page":"1364","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Prevalence and Characterization of Extended-Spectrum \u03b2-Lactamase- and Carbapenemase-Producing Enterobacterales from Tunisian Seafood"],"prefix":"10.3390","volume":"10","author":[{"given":"Mehdi","family":"Sola","sequence":"first","affiliation":[{"name":"Laboratoire de Recherche Biophysique M\u00e9tabolique et Pharmacologie Appliqu\u00e9e (LR12ES02), Facult\u00e9 de M\u00e9decine Ibn Al Jazzar Sousse, Universit\u00e9 de Sousse, Sousse 4002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosra","family":"Mani","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche Biophysique M\u00e9tabolique et Pharmacologie Appliqu\u00e9e (LR12ES02), Facult\u00e9 de M\u00e9decine Ibn Al Jazzar Sousse, Universit\u00e9 de Sousse, Sousse 4002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Saras","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes, ANSES Laboratoire de Lyon, Universit\u00e9 de Lyon, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Drapeau","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes, ANSES Laboratoire de Lyon, Universit\u00e9 de Lyon, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raoudha","family":"Grami","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche Biophysique M\u00e9tabolique et Pharmacologie Appliqu\u00e9e (LR12ES02), Facult\u00e9 de M\u00e9decine Ibn Al Jazzar Sousse, Universit\u00e9 de Sousse, Sousse 4002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahjoub","family":"Aouni","sequence":"additional","affiliation":[{"name":"Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Facult\u00e9 de Pharmacie de Monastir, Universit\u00e9 de Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Madec","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes, ANSES Laboratoire de Lyon, Universit\u00e9 de Lyon, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1333-5603","authenticated-orcid":false,"given":"Marisa","family":"Haenni","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes, ANSES Laboratoire de Lyon, Universit\u00e9 de Lyon, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wejdene","family":"Mansour","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche Biophysique M\u00e9tabolique et Pharmacologie Appliqu\u00e9e (LR12ES02), Facult\u00e9 de M\u00e9decine Ibn Al Jazzar Sousse, Universit\u00e9 de Sousse, Sousse 4002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"592291","DOI":"10.3389\/fmicb.2021.592291","article-title":"Plasmid-borne and chromosomal ESBL\/AmpC genes in Escherichia coli and Klebsiella pneumoniae in global food products","volume":"12","author":"Kurittu","year":"2021","journal-title":"Front. 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