{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T21:20:31Z","timestamp":1776374431159,"version":"3.51.2"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Red Cross","award":["FNS-31003A_163432"],"award-info":[{"award-number":["FNS-31003A_163432"]}]},{"name":"Portuguese Red Cross","award":["FNS-407240_177381"],"award-info":[{"award-number":["FNS-407240_177381"]}]},{"name":"University of Fribourg","award":["FNS-31003A_163432"],"award-info":[{"award-number":["FNS-31003A_163432"]}]},{"name":"University of Fribourg","award":["FNS-407240_177381"],"award-info":[{"award-number":["FNS-407240_177381"]}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["FNS-31003A_163432"],"award-info":[{"award-number":["FNS-31003A_163432"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["FNS-407240_177381"],"award-info":[{"award-number":["FNS-407240_177381"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This study aimed to investigate, for the first time, the occurrence and characteristics of extended-spectrum \u03b2-lactamase (ESBL)- and carbapenemase-producing Enterobacterales in Cape Verde. A total of 98 inpatients hospitalized at Hospital Universit\u00e1rio Agostinho Neto were screened for rectal colonization. All ESBL- and carbapenemase-producing isolates were tested for antimicrobial susceptibility and characterized by multilocus sequence typing. Mating-out assay followed by PCR-based replicon typing were performed to characterize the plasmids harboring carbapenemase encoding genes. A large proportion of patients carried ESBL- or carbapenemase-producing Enterobacterales (56% and 6%, respectively). Among 93 ESBL-producing isolates, there were mainly Klebsiella pneumoniae (58%) and Escherichia coli (37%). Five different ESBLs were detected, with CTX-M-15 being highly predominant (92%). Six carbapenemase-producing isolates (five E. coli and one K. pneumoniae) were recovered, and all of the OXA-48-like type (four OXA-181, one OXA-48, and one OXA-244). The blaOXA-48 gene was located on an IncFI-type plasmid, the blaOXA-181 gene on IncFI or IncX3 plasmids, and the blaOXA-244 gene was found to be chromosomally located. The five carbapenemase-producing E. coli isolates belonged to five distinct sequence types. This study overall showed a very high prevalence of ESBL-producing Enterobacterales, as well as the emergence of carbapenemase producers in this hospital.<\/jats:p>","DOI":"10.3390\/microorganisms10071426","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T01:57:11Z","timestamp":1657850231000},"page":"1426","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde"],"prefix":"10.3390","volume":"10","author":[{"given":"Samanta","family":"Freire","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Grilo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lu\u00edsa","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio Agostinho Neto, Praia 112, Cape Verde"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Euclides","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio Agostinho Neto, Praia 112, Cape Verde"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5160-5286","authenticated-orcid":false,"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Medical and Molecular Microbiology Unit, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, Switzerland"},{"name":"INSERM European Unit (IAME, France), University of Fribourg, 1700 Fribourg, Switzerland"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), 1700 Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5036-2446","authenticated-orcid":false,"given":"Marta","family":"Aires-de-Sousa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, Portugal"},{"name":"Escola Superior de Sa\u00fade da Cruz Vermelha Portuguesa-Lisboa (ESSCVP-Lisboa), 1300-125 Lisboa, Portugal"},{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), Universidade Nova de Lisboa (UNL), 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,14]]},"reference":[{"key":"ref_1","unstructured":"(2022, June 23). 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