{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:41:25Z","timestamp":1768783285885,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"The National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFB1503801"],"award-info":[{"award-number":["2019YFB1503801"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"The National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020WK2019"],"award-info":[{"award-number":["2020WK2019"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"The National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019CB1002"],"award-info":[{"award-number":["2019CB1002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Key Research and Development Program of Hunan Province","award":["2019YFB1503801"],"award-info":[{"award-number":["2019YFB1503801"]}]},{"name":"The Key Research and Development Program of Hunan Province","award":["2020WK2019"],"award-info":[{"award-number":["2020WK2019"]}]},{"name":"The Key Research and Development Program of Hunan Province","award":["2019CB1002"],"award-info":[{"award-number":["2019CB1002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The pretreatment of lignocellulosic biomass is important for efficient bioethanol conversion, but causes undesirable by-products that inhibit microbial growth, conversely affecting the bioconversion efficiency. In this study, the main inhibitors derived from dilute phosphoric acid plus steam-exploded poplar wood were identified as 0.22 g\/L furfural, 3.63 g\/L acetic acid, 0.08 g\/L syringaldehyde, etc., indicating the green nature and low toxicity of the pretreatment process. The effects of the three typical inhibitors (furfural, acetic acid, and syringaldehyde) on Saccharomyces cerevisiae 1517RM growth were analyzed and shown to prolong the lag phase of microbial growth to different degrees. In all the inhibitor groups, the ergosterol secretion was boosted, indicating low cell membrane fluidity and robustness of the strain to an adverse environment. The cell electronegativity and morphology of S. cerevisiae 1517RM also changed under different growth conditions, which was helpful for monitoring the physicochemical properties of cells. Furfural, acetic acid, and syringaldehyde had a synergistic effect on each other, providing an important reference to improving the subsequent ethanol fermentation process.<\/jats:p>","DOI":"10.3390\/microorganisms10071456","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T05:37:53Z","timestamp":1658209073000},"page":"1456","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of Inhibitors Generated by Dilute Phosphoric Acid Plus Steam-Exploded Poplar on Saccharomyces cerevisiae Growth"],"prefix":"10.3390","volume":"10","author":[{"given":"Yanan","family":"Wang","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"},{"name":"Hunan International Joint Laboratory of Woody Biomass Conversion, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zhan","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"},{"name":"Hunan International Joint Laboratory of Woody Biomass Conversion, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lishu","family":"Shao","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"},{"name":"Hunan International Joint Laboratory of Woody Biomass Conversion, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"},{"name":"Hunan International Joint Laboratory of Woody Biomass Conversion, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Qing","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"},{"name":"Hunan International Joint Laboratory of Woody Biomass Conversion, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121086","DOI":"10.1016\/j.fuel.2021.121086","article-title":"Lignocellulosic biomass based biorefinery: A successful platform towards circular bioeconomy","volume":"302","author":"Banu","year":"2021","journal-title":"Fuel"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"128523","DOI":"10.1016\/j.chemosphere.2020.128523","article-title":"A review on the environment-friendly emerging techniques for pretreatment of lignocellulosic biomass Mechanistic insight and advancements","volume":"264","author":"Haldar","year":"2021","journal-title":"Chemosphere"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2593","DOI":"10.1021\/ie503151g","article-title":"Application of Steam Explosion as Pretreatment on Lignocellulosic Material\u2014A Review","volume":"54","author":"Jacquet","year":"2015","journal-title":"Ind. 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