{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:19:05Z","timestamp":1788365945303,"version":"build-2803163510"},"reference-count":72,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"\u201cS\u00e3o Paulo State Research Support Foundation\u201d","doi-asserted-by":"publisher","award":["2018\/02753-0"],"award-info":[{"award-number":["2018\/02753-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"\u201cS\u00e3o Paulo State Research Support Foundation\u201d","doi-asserted-by":"publisher","award":["2018\/19672-2"],"award-info":[{"award-number":["2018\/19672-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"R.B.A.\u2019s Doctorate Scholarship","doi-asserted-by":"publisher","award":["2018\/02753-0"],"award-info":[{"award-number":["2018\/02753-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"R.B.A.\u2019s Doctorate Scholarship","doi-asserted-by":"publisher","award":["2018\/19672-2"],"award-info":[{"award-number":["2018\/19672-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The genus Bartonella (Rhizobiales: Bartonellaceae) encompasses facultative intracellular Gram-negative alphaproteobacteria that parasitize mainly erythrocytes and endothelial cells, as well as macrophages, monocytes and dendritic cells. Although they can infect numerous mammal species and arthropod vectors worldwide, reports of Bartonella infections in marsupials are scarce. In fact, such agents have only been detected in marsupials and\/or associated ectoparasites in Australia and the United States of America until the present moment. The present study aimed to isolate and characterize molecularly, morphologically and phenotypically Bartonella infecting free-living marsupials sampled in the Brazilian Pantanal, the largest wetland in South America. Two marsupials were captured in December 2018 and six marsupials in February 2019, totaling eight small mammals sampled: five (62.5%) Thylamys macrurus and three (37.5%) Monodelphis domestica. All blood samples were submitted to qPCR for Bartonella spp. based on the nuoG gene, a pre-enrichment liquid culture and a chocolate agar solid culture. Bartonella sp. was isolated from 3 T. macrurus and one M. domestica. One Bartonella isolate obtained from a T. macrurus blood sample (strain 117A) that showed to be closely related to the Bartonella vinsonii complex and Bartonella machadoae was selected for whole genome sequencing using a hybrid approach based on Illumina NovaSeq and Nanopore sequencing platforms. This strain showed a genome of 2.35 Mbp, with an average C + G content of 38.8%, coding for 2013 genes, and a 29 kb plasmid with an average C + G content of 34.5%. In addition, this strain exhibited an average nucleotide identity (ANI) of 85% with Bartonella species belonging to the B. vinsonii group and 91% with B. machadoae. Phylogenomic analysis based on 291 protein coding genes shared by the genomes of 53 Bartonella species positioned this strain closely to B. machadoae. This new isolated species was named Bartonella harrusi sp. nov., which was characterized as having small capnophilic, microaerophilic and aerobic rods with an absence of pili and flagella. In conclusion, the present work describes the biochemical, phenotypic and genomic characteristics of Bartonella harrusi, a new species isolated from the T. macrurus blood samples of the Brazilian Pantanal. Finally, a review of the taxonomic classification of members of the genus Bartonella is proposed, based on the ANI values accessed by whole genome sequencing analyses.<\/jats:p>","DOI":"10.3390\/microorganisms10081609","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T04:20:32Z","timestamp":1660105232000},"page":"1609","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella"],"prefix":"10.3390","volume":"10","author":[{"given":"Renan Bressianini","family":"do Amaral","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 15385-000, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia, Reprodu\u00e7\u00e3o e Sa\u00fade \u00danica, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita Vedovelli","family":"Cardozo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia, Reprodu\u00e7\u00e3o e Sa\u00fade \u00danica, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8876-3269","authenticated-orcid":false,"given":"Alessandro de Mello","family":"Varani","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 15385-000, SP, Brazil"},{"name":"Departamento de Biotecnologia Agropecu\u00e1ria e Ambiental, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eduarda Chiaradia","family":"Furquim","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 15385-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9348-4814","authenticated-orcid":false,"given":"Clara Morato","family":"Dias","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 15385-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Oliveira de","family":"Assis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Parasit\u00e1ria, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alanderson Rodrigues","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Parasit\u00e1ria, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2404-8765","authenticated-orcid":false,"given":"Heitor Miraglia","family":"Herrera","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Parasit\u00e1ria, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5176-2940","authenticated-orcid":false,"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 15385-000, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia, Reprodu\u00e7\u00e3o e Sa\u00fade \u00danica, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agropecu\u00e1ria, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 15385-000, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunoparasitologia, Departamento de Patologia, Reprodu\u00e7\u00e3o e Sa\u00fade \u00danica, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.3201\/eid1203.050931","article-title":"Bartonella spp. in pets and effect on human health","volume":"12","author":"Chomel","year":"2006","journal-title":"Emerg. 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