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Our previous data reported the genus Methylocystis as one of the most abundant methanotrophs in these floodplain sediments. However, information on the functional potential and life strategies of these organisms living under seasonal flooding is still missing. Here, we described the first metagenome-assembled genome (MAG) of a Methylocystis sp. recovered from Amazonian floodplains sediments, and we explored its functional potential and ecological traits through phylogenomic, functional annotation, and pan-genomic approaches. Both phylogenomics and pan-genomics identified the closest placement of the bin.170_fp as Methylocystis parvus. As expected for Type II methanotrophs, the Core cluster from the pan-genome comprised genes for CH4 oxidation and formaldehyde assimilation through the serine pathway. Furthermore, the complete set of genes related to nitrogen fixation is also present in the Core. Interestingly, the MAG singleton cluster revealed the presence of unique genes related to nitrogen metabolism and cell motility. The study sheds light on the genomic characteristics of a dominant, but as yet unexplored methanotroph from the Amazonian floodplains. By exploring the genomic potential related to resource utilization and motility capability, we expanded our knowledge on the niche breadth of these dominant methanotrophs in the Amazonian floodplains.<\/jats:p>","DOI":"10.3390\/microorganisms10091747","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:13:56Z","timestamp":1661904836000},"page":"1747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Insights into the Genomic Potential of a Methylocystis sp. from Amazonian Floodplain Sediments"],"prefix":"10.3390","volume":"10","author":[{"given":"J\u00falia B.","family":"Gontijo","sequence":"first","affiliation":[{"name":"Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of S\u00e3o Paulo (CENA-USP), Piracicaba 13416-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana S.","family":"Paula","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of S\u00e3o Paulo (CENA-USP), Piracicaba 13416-000, Brazil"},{"name":"Department of Biological Oceanography, Oceanographic Institute, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5901-1658","authenticated-orcid":false,"given":"Andressa M.","family":"Venturini","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of S\u00e3o Paulo (CENA-USP), Piracicaba 13416-000, Brazil"},{"name":"Princeton Institute for International and Regional Studies, Princeton University, Princeton, NJ 08544-1019, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3454-0702","authenticated-orcid":false,"given":"J\u00e9ssica A.","family":"Mandro","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of S\u00e3o Paulo (CENA-USP), Piracicaba 13416-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5757-5572","authenticated-orcid":false,"given":"Paul L. E.","family":"Bodelier","sequence":"additional","affiliation":[{"name":"Netherlands Institute of Ecology (NIOO-KNAW), 6708 PB Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siu M.","family":"Tsai","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of S\u00e3o Paulo (CENA-USP), Piracicaba 13416-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1073\/pnas.1814297116","article-title":"Interpreting contemporary trends in atmospheric methane","volume":"116","author":"Turner","year":"2019","journal-title":"Proc. Natl. Acad. Sci. 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