{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T01:21:44Z","timestamp":1769044904336,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T00:00:00Z","timestamp":1662854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Project of China","award":["2021YFE0101700"],"award-info":[{"award-number":["2021YFE0101700"]}]},{"name":"National Key Research and Development Project of China","award":["82061148018"],"award-info":[{"award-number":["82061148018"]}]},{"name":"National Key Research and Development Project of China","award":["32170177"],"award-info":[{"award-number":["32170177"]}]},{"name":"National Key Research and Development Project of China","award":["32170199"],"award-info":[{"award-number":["32170199"]}]},{"name":"National Key Research and Development Project of China","award":["31970179"],"award-info":[{"award-number":["31970179"]}]},{"name":"National Key Research and Development Project of China","award":["31970680"],"award-info":[{"award-number":["31970680"]}]},{"name":"National Key Research and Development Project of China","award":["31900115"],"award-info":[{"award-number":["31900115"]}]},{"name":"National Key Research and Development Project of China","award":["63223030"],"award-info":[{"award-number":["63223030"]}]},{"name":"National Science Foundation of China","award":["2021YFE0101700"],"award-info":[{"award-number":["2021YFE0101700"]}]},{"name":"National Science Foundation of China","award":["82061148018"],"award-info":[{"award-number":["82061148018"]}]},{"name":"National Science Foundation of China","award":["32170177"],"award-info":[{"award-number":["32170177"]}]},{"name":"National Science Foundation of China","award":["32170199"],"award-info":[{"award-number":["32170199"]}]},{"name":"National Science Foundation of China","award":["31970179"],"award-info":[{"award-number":["31970179"]}]},{"name":"National Science Foundation of China","award":["31970680"],"award-info":[{"award-number":["31970680"]}]},{"name":"National Science Foundation of China","award":["31900115"],"award-info":[{"award-number":["31900115"]}]},{"name":"National Science Foundation of China","award":["63223030"],"award-info":[{"award-number":["63223030"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2021YFE0101700"],"award-info":[{"award-number":["2021YFE0101700"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["82061148018"],"award-info":[{"award-number":["82061148018"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["32170177"],"award-info":[{"award-number":["32170177"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["32170199"],"award-info":[{"award-number":["32170199"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["31970179"],"award-info":[{"award-number":["31970179"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["31970680"],"award-info":[{"award-number":["31970680"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["31900115"],"award-info":[{"award-number":["31900115"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["63223030"],"award-info":[{"award-number":["63223030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Multidrug-resistant (MDR) Pseudomonas aeruginosa poses a great challenge to clinical treatment. In this study, we characterized a ST768 MDR P. aeruginosa strain, Pa150, that was isolated from a diabetic foot patient. The minimum inhibitory concentration (MIC) assay showed that Pa150 was resistant to almost all kinds of antibiotics, especially aminoglycosides. Whole genome sequencing revealed multiple antibiotic resistant genes on the chromosome and a 437-Kb plasmid (named pTJPa150) that harbors conjugation-related genes. A conjugation assay verified its self-transmissibility. On the pTJPa150 plasmid, we identified a 16S rRNA methylase gene, rmtB, that is flanked by mobile genetic elements (MGEs). The transfer of the pTJPa150 plasmid or the cloning of the rmtB gene into the reference strain, PAO1, significantly increased the bacterial resistance to aminoglycoside antibiotics. To the best of our knowledge, this is the first report of an rmtB-carrying conjugative plasmid isolated from P. aeruginosa, revealing a novel possible transmission mechanism of the rmtB gene.<\/jats:p>","DOI":"10.3390\/microorganisms10091818","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T01:44:03Z","timestamp":1663033443000},"page":"1818","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Emergence and Transfer of Plasmid-Harbored rmtB in a Clinical Multidrug-Resistant Pseudomonas aeruginosa Strain"],"prefix":"10.3390","volume":"10","author":[{"given":"Jiacong","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoya","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwen","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxin","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0639-8104","authenticated-orcid":false,"given":"Fang","family":"Bai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihui","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9017-0091","authenticated-orcid":false,"given":"Weihui","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,11]]},"reference":[{"key":"ref_1","first-page":"2964","article-title":"Two Pseudomonas aeruginosa clonal groups belonging to the PA14 clade are indigenous to the Churince system in Cuatro Ci\u00e9negas Coahuila, M\u00e9xico","volume":"218","author":"Souza","year":"2019","journal-title":"Environ. 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