{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:44:14Z","timestamp":1760244254829,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Manitoba and Manitoba Medical Service Foundation (MMSF)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: We examined associations between NF\u03baB1 polymorphisms and influenza A (H1N1) clinical outcomes in Canadian. Methods: A total of thirty-six Caucasian patients admitted to the intensive care unit (ICU) in hospitals in Canada were recruited during the 2009 H1N1 pandemic. Genomic DNA was extracted from the whole blood samples. The NFkB1 gene was targeted for genotyping using next-generation sequencing technology\u2014Roche 454. Results: A total of 136 single nucleotide polymorphisms (SNPs) were discovered within the NF\u03baB1 gene. Among them, 63 SNPs were significantly enriched in patients admitted in the ICU (p &lt; 0.05) compared with the British Caucasian population in the 1000 Genomes study. These enriched SNPs are mainly intron variants, and only two are exon SNPs from the non-transcribing portion of the NF\u03baB1 gene. Conclusions: Genetic variations in the NF\u03baB1 gene could influence clinical outcomes of pandemic H1N1 infections. Our findings showed that sequence variations of the NF\u03baB1 gene might influence patient response to influenza infection.<\/jats:p>","DOI":"10.3390\/microorganisms10101886","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T05:33:14Z","timestamp":1663824794000},"page":"1886","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["NF\u03baB1 Polymorphisms Are Associated with Severe Influenza A (H1N1) Virus Infection in a Canadian Population"],"prefix":"10.3390","volume":"10","author":[{"given":"Suhrobjon Mullo","family":"Mirzo","sequence":"first","affiliation":[{"name":"Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3X 0P3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"},{"name":"Critical Care Medicine, Health Science Centre, University of Manitoba, Winnipeg, MB R3A 1R9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naresh Kumar","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"JC Wilt Infectious Diseases Research Centre, National Microbiology Laboratory, Winnipeg, MB R3E 3L5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2455-8792","authenticated-orcid":false,"given":"Robert","family":"Balshaw","sequence":"additional","affiliation":[{"name":"Data Sciences Platform, George & Fay Yee Centre for Healthcare Innovation, University of Manitoba, Winnipeg, MB R3X 0T6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis A.","family":"Plummer","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"},{"name":"JC Wilt Infectious Diseases Research Centre, National Microbiology Laboratory, Winnipeg, MB R3E 3L5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binhua","family":"Liang","sequence":"additional","affiliation":[{"name":"JC Wilt Infectious Diseases Research Centre, National Microbiology Laboratory, Winnipeg, MB R3E 3L5, Canada"},{"name":"Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Writing Committee of the WHO Consultation on Clinical Aspects of Pandemic (H1N1) 2009 Influenza (2010). 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