{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:11:31Z","timestamp":1762067491578,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Academician Innovation Platform Project of Hainan Province","award":["YSPTZX202013","CARS-38","ZY2022HN09"],"award-info":[{"award-number":["YSPTZX202013","CARS-38","ZY2022HN09"]}]},{"name":"Hainan Innovation Center for Academician of Jin Ningyi","award":["YSPTZX202013","CARS-38","ZY2022HN09"],"award-info":[{"award-number":["YSPTZX202013","CARS-38","ZY2022HN09"]}]},{"name":"Hainan Innovation Center for Academician of Zhang Yong","award":["YSPTZX202013","CARS-38","ZY2022HN09"],"award-info":[{"award-number":["YSPTZX202013","CARS-38","ZY2022HN09"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["YSPTZX202013","CARS-38","ZY2022HN09"],"award-info":[{"award-number":["YSPTZX202013","CARS-38","ZY2022HN09"]}]},{"name":"Special Funding Projects for Local Science and Technology Development Guided by the Central Committee","award":["YSPTZX202013","CARS-38","ZY2022HN09"],"award-info":[{"award-number":["YSPTZX202013","CARS-38","ZY2022HN09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Mycoplasma mycoides subspecies capri (Mmc) is one of the six Mycoplasma mycoides cluster (Mm cluster) members, which can cause \u201cMAKePS\u201d (Mastitis, Arthritis, Keratoconjunctivitis, Pneumonia, Septicemia) syndrome in ruminants. These symptoms can occur alone or together in individuals or flocks of goats. However, little is known about the epidemic Mmc strains in Hainan Island, China. We aimed to isolate the endemic Mmc strains in Hainan Island and reveal their molecular characteristics by genomic sequencing and comparative genomics to mitigate the impact of Mmc on local ruminant farming. Here, the Mmc HN-A strain was isolated and identified for the first time in Hainan Island, China. The genome of Mmc HN-A was sequenced. It contains a 1,084,691 bp-long circular chromosome and 848 coding genes. The genomic analysis of Mmc HN-A revealed 16 virulence factors, 2 gene islands, and a bacterial type IV secretion system protein VirD4. Comparative genomics showed that the core genome of the five Mycoplasma mycoides contained 611 genes that could be exploited to develop drugs and endemic vaccines. Additionally, 36 specific genes were included in the Mmc HN-A genome, which could provide the possibility for the further control and prevention of the Mmc effects on local ruminants and enrich the information on Mmc strains.<\/jats:p>","DOI":"10.3390\/microorganisms10101908","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T01:51:49Z","timestamp":1664329909000},"page":"1908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["First Report and Comparative Genomic Analysis of a Mycoplasma mycoides Subspecies capri HN-A in Hainan Island"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3205-5909","authenticated-orcid":false,"given":"Zhenxing","family":"Zhang","sequence":"first","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junming","family":"Jiang","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meirong","family":"He","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoyang","family":"Li","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiwen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"An","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0435-1954","authenticated-orcid":false,"given":"Si","family":"Chen","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Du","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Churiga","family":"Man","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4442-9450","authenticated-orcid":false,"given":"Qiaoling","family":"Chen","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianbin","family":"Li","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.jmii.2020.06.002","article-title":"The First Isolation and Molecular Characterization of Mycoplasma capricolum subsp. capripneumoniae Pakistan Strain: A Causative Agent of Contagious Caprine Pleuropneumonia","volume":"54","author":"Ahmad","year":"2021","journal-title":"J. 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