{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:24Z","timestamp":1787028984413,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2016R1C1B108888"],"award-info":[{"award-number":["NRF-2016R1C1B108888"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2020R1C1007169"],"award-info":[{"award-number":["NRF-2020R1C1007169"]}]},{"name":"Brain Busan 21 Plus project","award":["NRF-2016R1C1B108888"],"award-info":[{"award-number":["NRF-2016R1C1B108888"]}]},{"name":"Brain Busan 21 Plus project","award":["NRF-2020R1C1007169"],"award-info":[{"award-number":["NRF-2020R1C1007169"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Tuberculosis (TB) management is important for prompt discrimination of latent TB infection (LTBI) from active TB and proper treatment. Whole blood Interferon-gamma (IFN-\u03b3) release assay (IGRA) is used to diagnose LTBI based on the secretion of IFN-\u03b3 by T-cells in the whole blood by using a specific antigen of Mycobacterium tuberculosis. However, the ability of IGRA to distinguish active TB from LTBI is considerably limited. Distinguishing active TB from LTBI is necessary to identify indicators that can be used to effectively manage TB and develop diagnostic methods. In the present study, we used a Luminex multiplex bead array (a bead-based antibody\u2013antigen sandwich method). The whole blood level of acute phase proteins (APPs), such as endoglin (ENG), procalcitonin (PCT), C-reactive protein (CRP), and \u03b11-acid glycoprotein (AGP), in active TB, LTBI, and healthy individuals were analyzed and quantified. The APP test results for the serum and whole blood samples showed that the levels of PCT, CRP, and AGP were significantly increased (p &lt; 0.0500; area under curve = 0.955) in active TB. The level of these markers in the whole blood of active TB, LTBI, and healthy individuals could provide data for effective diagnosis and treatment of TB.<\/jats:p>","DOI":"10.3390\/microorganisms10101928","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T22:53:19Z","timestamp":1664405599000},"page":"1928","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["High Procalcitonin, C-Reactive Protein, and \u03b1-1 Acid Glycoprotein Levels in Whole Blood Samples Could Help Rapid Discrimination of Active Tuberculosis from Latent Tuberculosis Infection and Healthy Individuals"],"prefix":"10.3390","volume":"10","author":[{"given":"Yun-Jeong","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Department of Laboratory Medicine, Good Samsun Hospital, Busan 47007, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heechul","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Bae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, Masan University, Changwon 51217, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Institute of Dream DX Inc., Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyanglan","family":"Hyun","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minju","family":"Jung","sequence":"additional","affiliation":[{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Department of Forensic Science, Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Ju","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-A","family":"Je","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Sung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Good Samsun Hospital, Busan 47007, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2511-6555","authenticated-orcid":false,"given":"Sunghyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.cyto.2016.08.030","article-title":"Identification of promising plasma immune biomarkers to differentiate active pulmonary tuberculosis","volume":"88","author":"Zambuzi","year":"2016","journal-title":"Cytokine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/1751-7915.13291","article-title":"Identification of new diagnostic biomarkers for Mycobacterium tuberculosis and the potential application in the serodiagnosis of human tuberculosis","volume":"11","author":"Ren","year":"2018","journal-title":"Microb. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e2018036","DOI":"10.4178\/epih.e2018036","article-title":"Tuberculosis control in the Republic of Korea","volume":"40","author":"Cho","year":"2018","journal-title":"Epidemiol. Health"},{"key":"ref_4","first-page":"515","article-title":"Screening of Serum Biomarkers for Distinguishing between Latent and Active Tuberculosis Using Proteome Microarray","volume":"31","author":"Cao","year":"2018","journal-title":"Biomed. Environ. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.tube.2015.04.003","article-title":"Latent tuberculosis infection\u2014Revisiting and revising concepts","volume":"95","author":"Salgame","year":"2015","journal-title":"Tuberculosis"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s40291-014-0125-0","article-title":"A systematic review on TST and IGRA tests used for diagnosis of LTBI in immigrants","volume":"19","author":"Campbell","year":"2015","journal-title":"Mol. Diagn. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6263","DOI":"10.1007\/s11033-019-05067-0","article-title":"Diagnostic accuracy of interferon (IFN)-gamma inducible protein 10 (IP-10) as a biomarker for the discrimination of active and latent tuberculosis","volume":"46","author":"Mamishi","year":"2019","journal-title":"Mol. Biol. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lee, S.W., Wu, L.S., Huang, G.M., Huang, K.Y., Lee, T.Y., and Weng, J.T. (2016). Gene expression profiling identifies candidate biomarkers for active and latent tuberculosis. BMC Bioinform., 17.","DOI":"10.1186\/s12859-015-0848-x"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.jinf.2016.11.010","article-title":"Biomarkers for discrimination between latent tuberculosis infection and active tuberculosis disease","volume":"74","author":"Won","year":"2017","journal-title":"J. Infect."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sun, T., Wu, B., Wang, J., Yuan, T., Huang, H., Xu, D., and Deng, S. (2019). Evaluation of the Diagnostic Efficacy of Monocyte Parameters and MCP-1 to Distinguishing Active Tuberculosis from Latent Tuberculosis. Clin. Lab., 65.","DOI":"10.7754\/Clin.Lab.2018.181115"},{"key":"ref_11","first-page":"2077","article-title":"Comparative proteomic analysis of serum diagnosis patterns of sputum smear-positive pulmonary tuberculosis based on magnetic bead separation and mass spectrometry analysis","volume":"8","author":"Liu","year":"2015","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/pmic.201400366","article-title":"Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ-coupled two-dimensional LC-MS\/MS","volume":"15","author":"Xu","year":"2015","journal-title":"Proteomics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.3389\/fmicb.2018.01267","article-title":"Label-Free Quantitative Proteomics Identifies Novel Plasma Biomarkers for Distinguishing Pulmonary Tuberculosis and Latent Infection","volume":"9","author":"Sun","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.cmi.2018.07.017","article-title":"Acute phase proteins and IP-10 as triage tests for the diagnosis of tuberculosis: Systematic review and meta-analysis","volume":"25","author":"Santos","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Stavrum, R., PrayGod, G., Range, N., Faurholt-Jepsen, D., Jeremiah, K., Faurholt-Jepsen, M., Krarup, H., Aabye, M.G., Changalucha, J., and Friis, H. (2014). Increased level of acute phase reactants in patients infected with modern Mycobacterium tuberculosis genotypes in Mwanza, Tanzania. BMC Infect. Dis., 14.","DOI":"10.1186\/1471-2334-14-309"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.tube.2015.04.011","article-title":"Serum biomarkers of treatment response within a randomized clinical trial for pulmonary tuberculosis","volume":"95","author":"Jayakumar","year":"2015","journal-title":"Tuberculosis"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ruiz-Remolina, L., Ollauri-Ibanez, C., Perez-Roque, L., Nunez-Gomez, E., Perez-Barriocanal, F., Lopez-Novoa, J.M., Pericacho, M., and Rodriguez-Barbero, A. (2017). Circulating soluble endoglin modifies the inflammatory response in mice. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0188204"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"330","DOI":"10.3389\/fmicb.2018.00330","article-title":"Identification of a Novel Serum Biomarker for Tuberculosis Infection in Chinese HIV Patients by iTRAQ-Based Quantitative Proteomics","volume":"9","author":"Chen","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.5588\/ijtld.17.0078","article-title":"Diagnostic accuracy of C-reactive protein for active pulmonary tuberculosis: A meta-analysis","volume":"21","author":"Yoon","year":"2017","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.tube.2007.10.004","article-title":"Alpha-1-acid glycoprotein, its local production and immunopathological participation in experimental pulmonary tuberculosis","volume":"88","author":"Gonzalez","year":"2008","journal-title":"Tuberculosis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e12177","DOI":"10.1097\/MD.0000000000012177","article-title":"Effect of linezolid on serum PCT, ESR, and CRP in patients with pulmonary tuberculosis and pneumonia","volume":"97","author":"Ding","year":"2018","journal-title":"Medicine"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"284","DOI":"10.3904\/kjm.2012.82.3.284","article-title":"Diagnosis and Treatment of Latent Tuberculosis Infection","volume":"82","author":"Shim","year":"2012","journal-title":"Korean J. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"263","DOI":"10.3904\/kjm.2012.82.3.263","article-title":"New Diagnostic Methods for Tuberculosis","volume":"82","author":"Lim","year":"2012","journal-title":"Korean J. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1086\/589248","article-title":"Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum","volume":"47","author":"Marais","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11097","DOI":"10.1038\/s41598-022-15482-2","article-title":"Role of GBP1 in innate immunity and potential as a tuberculosis biomarker","volume":"12","author":"Shi","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_26","unstructured":"WHO (2021). Global Tuberculosis Report 2021, WHO."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sharma, S.K., Vashishtha, R., Chauhan, L.S., Sreenivas, V., and Seth, D. (2017). Comparison of TST and IGRA in Diagnosis of Latent Tuberculosis Infection in a High TB-Burden Setting. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169539"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Schoonderwoerd, M.J.A., Goumans, M.T.H., and Hawinkels, L. (2020). Endoglin: Beyond the Endothelium. Biomolecules, 10.","DOI":"10.3390\/biom10020289"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1056\/NEJMra0706596","article-title":"Tumor angiogenesis","volume":"358","author":"Kerbel","year":"2008","journal-title":"N. Engl. J. Med."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mossenta, M., Busato, D., Baboci, L., Cintio, F.D., Toffoli, G., and Bo, M.D. (2019). New Insight into Therapies Targeting Angiogenesis in Hepatocellular Carcinoma. Cancers, 11.","DOI":"10.3390\/cancers11081086"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Jeng, K.S., Sheen, I.S., Lin, S.S., Leu, C.M., and Chang, C.F. (2021). The Role of Endoglin in Hepatocellular Carcinoma. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22063208"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6331","DOI":"10.1158\/1078-0432.CCR-18-0329","article-title":"Endoglin Expression on Cancer-Associated Fibroblasts Regulates Invasion and Stimulates Colorectal Cancer Metastasis","volume":"24","author":"Paauwe","year":"2018","journal-title":"Clin. Cancer Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1016\/j.ijbiomac.2019.10.096","article-title":"Interaction of antitubercular drug candidates with alpha1-acid glycoprotein produced in pulmonary granulomas","volume":"147","author":"Zsila","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/0167-5699(94)90137-6","article-title":"The acute phase response","volume":"15","author":"Baumann","year":"1994","journal-title":"Immunol. Today"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1089\/ham.2018.0017","article-title":"Urinary Alpha-1-Acid Glycoprotein Is a Sensitive Marker of Glomerular Protein Leakage at Altitude","volume":"19","author":"Talks","year":"2018","journal-title":"High Alt. Med. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1136\/jclinpath-2014-202807","article-title":"Procalcitonin","volume":"68","author":"Davies","year":"2015","journal-title":"J. Clin. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1159\/000438557","article-title":"Serum Procalcitonin Level and Mortality Risk in Critically ill Patients with Ventilator-Associated Pneumonia","volume":"37","author":"Li","year":"2015","journal-title":"Cell Physiol. Biochem."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/10\/1928\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:41:09Z","timestamp":1760143269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/10\/1928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,28]]},"references-count":37,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["microorganisms10101928"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10101928","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,28]]}}}