{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:44:16Z","timestamp":1760244256574,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health, Rome, Italy","award":["RC 2022, Linea 4E"],"award-info":[{"award-number":["RC 2022, Linea 4E"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Theoretically, Aspergillus spp. grow in culture media, but frequently, blood cultures of patients with invasive Aspergillosis are negative, even if until now, the reasons are not clear. This aspect underlines the lack of a good strategy for the cultivation and isolation of Aspergillus spp. In order to develop a complete analytical method to detect Aspergillus in clinical and pharmaceutical samples, we investigated the growth performance of two blood culture systems versus the pharmacopeia standard method. At &lt;72 h, all test systems showed comparable sensitivity, about 1\u20132 conidia. However, the subculture analysis showed a suboptimal recovery for the methods, despite the positive growth and the visualization of the \u201cAspergillus balls\u201d in the culture media. To investigate this issue, we studied three different subculture approaches: (i) the use of a sterile subculture unit, (ii) the use of a sterile subculture unit and the collection of a larger aliquot (100 \u00b5L), following vigorous agitation of the vials, and (iii) to decapsulate the bottle, withdrawing and centrifuging the sample, and aliquot the pellet onto SDA plates. Our results showed that only the third procedure recovered Aspergillus from all positive culture bottles. This work confirmed that our strategy is a valid and faster method to culture and isolate Aspergillus spp. from blood culture bottles.<\/jats:p>","DOI":"10.3390\/microorganisms10102026","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T22:21:11Z","timestamp":1665699671000},"page":"2026","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Growth Performance and Recovery of Nosocomial Aspergillus spp. in Blood Culture Bottles"],"prefix":"10.3390","volume":"10","author":[{"given":"Salvatore","family":"Pasqua","sequence":"first","affiliation":[{"name":"Unit\u00e0 Prodotti Cellulari (GMP), Fondazione Ri.MED c\/o IRCCS-ISMETT, Via E. Tricomi 5, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1415-5911","authenticated-orcid":false,"given":"Francesco","family":"Monaco","sequence":"additional","affiliation":[{"name":"Unit\u00e0 di Medicina di Laboratorio e Biotecnologie Avanzate, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Via E. Tricomi 5, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Cardinale","sequence":"additional","affiliation":[{"name":"Unit\u00e0 di Medicina di Laboratorio e Biotecnologie Avanzate, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Via E. Tricomi 5, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Bonelli","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy"},{"name":"Proteomics Group of Fondazione Ri.MED, Department of Research IRCCS ISMETT, Via Ernesto Tricomi 5, 90145 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1994-8005","authenticated-orcid":false,"given":"Pier Giulio","family":"Conaldi","sequence":"additional","affiliation":[{"name":"Unit\u00e0 di Medicina di Laboratorio e Biotecnologie Avanzate, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Via E. Tricomi 5, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6215-4182","authenticated-orcid":false,"given":"Danilo","family":"D\u2019Apolito","sequence":"additional","affiliation":[{"name":"Unit\u00e0 Prodotti Cellulari (GMP), Fondazione Ri.MED c\/o IRCCS-ISMETT, Via E. Tricomi 5, 90127 Palermo, Italy"},{"name":"Unit\u00e0 di Medicina di Laboratorio e Biotecnologie Avanzate, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Via E. Tricomi 5, 90127 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.idc.2021.03.008","article-title":"Aspergillosis: Epidemiology, Diagnosis, and Treatment","volume":"35","author":"Cadena","year":"2021","journal-title":"Infect. Dis. Clin. 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