{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:06:53Z","timestamp":1791230813629,"version":"4.1.0"},"reference-count":72,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T00:00:00Z","timestamp":1666828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Programme","award":["773830"],"award-info":[{"award-number":["773830"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Human\u2013wildlife coexistence may increase the potential risk of direct transmission of emergent or re-emergent zoonotic pathogens to humans. Intending to assess the occurrence of three important foodborne pathogens in wild animals of two wildlife conservation centers in Portugal, we investigated 132 fecal samples for the presence of Escherichia coli (Shiga toxin-producing E. coli (STEC) and non-STEC), Salmonella spp. and Campylobacter spp. A genotypic search for genes having virulence and antimicrobial resistance (AMR) was performed by means of PCR and Whole-Genome Sequencing (WGS) and phenotypic (serotyping and AMR profiles) characterization. Overall, 62 samples tested positive for at least one of these species: 27.3% for STEC, 11.4% for non-STEC, 3.0% for Salmonella spp. and 6.8% for Campylobacter spp. AMR was detected in four E. coli isolates and the only Campylobacter coli isolated in this study. WGS analysis revealed that 57.7% (30\/52) of pathogenic E. coli integrated genetic clusters of highly closely related isolates (often involving different animal species), supporting the circulation and transmission of different pathogenic E. coli strains in the studied areas. These results support the idea that the health of humans, animals and ecosystems are interconnected, reinforcing the importance of a One Health approach to better monitor and control public health threats.<\/jats:p>","DOI":"10.3390\/microorganisms10112132","type":"journal-article","created":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T22:36:17Z","timestamp":1666910177000},"page":"2132","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9219-4222","authenticated-orcid":false,"given":"Angela","family":"Pista","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8485-3008","authenticated-orcid":false,"given":"Leonor","family":"Silveira","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Fontes","sequence":"additional","affiliation":[{"name":"National 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Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3767-2209","authenticated-orcid":false,"given":"V\u00edtor","family":"Borges","sequence":"additional","affiliation":[{"name":"Genomics and Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"S\u00e1","sequence":"additional","affiliation":[{"name":"Tapada Nacional de Mafra, Port\u00e3o do Code\u00e7al, 2640-602 Mafra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Centro de Recupera\u00e7\u00e3o do Parque Biol\u00f3gico de Gaia, Rua da Cunha, Avintes, 4430-812 Vila Nova de Gaia, 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Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.3201\/eid1112.050997","article-title":"Host range and emerging and reemerging pathogens","volume":"11","author":"Woolhouse","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Martins, S.B., H\u00e4sler, B., and Rushton, J. (2014). Economic Aspects of Zoonoses: Impact of Zoonoses on the Food Industry. Zoonoses-Infections Affecting Humans and Animals, Springer.","DOI":"10.1007\/978-94-017-9457-2_45"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control) (2021). The European Union One Health 2020 Zoonoses Report. EFSA J., 19, 6971.","DOI":"10.2903\/j.efsa.2021.6971"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1007\/s10393-019-01405-7","article-title":"The One Health Approach to Toxoplasmosis: Epidemiology, Control, and Prevention Strategies","volume":"16","author":"Aguirre","year":"2019","journal-title":"Ecohealth"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.4102\/ojvr.v87i1.1895","article-title":"Zoonotic origins and animal hosts of coronaviruses causing human disease pandemics: A review","volume":"87","author":"Latif","year":"2020","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1002\/ps.3965","article-title":"Wild boar populations up, numbers of hunters down? A review of trends and implications for Europe","volume":"71","author":"Massei","year":"2015","journal-title":"Pest Manag. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control) (2021). The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2018\/2019. EFSA J., 19, 6490.","DOI":"10.2903\/j.efsa.2021.6490"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1128\/AEM.07520-11","article-title":"Seropathotypes, Phylogroups, Stx subtypes, and intimin types of wildlife-carried, shiga toxin-producing Escherichia coli strains with the same characteristics as human-pathogenic isolates","volume":"78","author":"Mora","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Bertelloni, F., Cilia, G., Bogi, S., Ebani, V.V., Turini, L., Nuvoloni, R., Cerri, D., Fratini, F., and Turchi, B. (2020). Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany. Animals, 10.","DOI":"10.3390\/ani10040744"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.vetmic.2017.05.028","article-title":"Occurrence;and characterization of stx and\/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar","volume":"207","author":"Alonso","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2542","DOI":"10.1017\/S0950268814000119","article-title":"Presence of Salmonella spp., Yersinia enterocolitica, Yersinia pseudotuberculosis and Escherichia coli O157:H7 in wild boars","volume":"142","author":"Hestvik","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.vetmic.2009.11.016","article-title":"Detection and characterisation of O157:H7 and non-O157 Shiga toxin-producing Escherichia coli in wild boars","volume":"143","author":"Martinez","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1080\/01652176.2015.1023404","article-title":"Escherichia coli O157:H7 in wild boars (Sus scrofa) and Iberian ibex (Capra pyrenaica) sharing pastures with free-ranging livestock in a natural environment in Spain","volume":"35","author":"Porrero","year":"2015","journal-title":"Vet. Q"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.scitotenv.2018.09.162","article-title":"Shiga toxin-producing Escherichia coli in wild ungulates","volume":"651","author":"Dias","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ijfoodmicro.2019.04.004","article-title":"Pathogenic potential to humans of Shiga toxin-producing Escherichia coli isolated from wild boars in Poland","volume":"300","author":"Socha","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.vetmic.2013.07.037","article-title":"Food-borne zoonotic pathogens and antimicrobial resistance of indicator bacteria in urban wild boars in Barcelona, Spain","volume":"167","author":"Porrero","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1089\/fpd.2009.0367","article-title":"Wild Boars as an Important Reservoir for Foodborne Pathogens","volume":"7","author":"Wacheck","year":"2010","journal-title":"Foodborne Pathog Dis"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"592512","DOI":"10.3389\/fcimb.2020.592512","article-title":"Genotyping and Antibiotic Resistance Traits in Campylobacter jejuni and coli from Pigs and Wild Boars in Italy","volume":"10","author":"Marotta","year":"2020","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"20 Mokracka, J., Krzymi\u0144ska, S., A\u0142tunin, D., Wasyl, D., Koczura, R., Dudek, K., Dudek, M., Chyle\u0144ska, Z.A., and Ekner-Grzyb, A. (2018). In vitro virulence characteristics of rare serovars of Salmonella enterica isolated from sand lizards (Lacerta agilis L.). Antonie Van Leeuwenhoek, 111, 1863\u20131870.","DOI":"10.1007\/s10482-018-1079-8"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1128\/AEM.66.2.844-849.2000","article-title":"Selective and sensitive method for PCR amplification of Escherichia coli 16S rRNA genes in soil","volume":"66","author":"Sabat","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","unstructured":"(2017). Microbiology of the Food Chain-Horizontal Method for the Detection, Enumeration and Serotyping of Salmonella\u2014Part 1: Detection of Salmonella spp.. Standard No. ISO 6579-1:2017."},{"key":"ref_23","unstructured":"(2006). Microbiology of Food and Animal Feeding Stuffs\u2014Horizontal Method for Detection and Enumeration of Campylobacter spp.\u2014Part 1: Detection Method. Standard No. ISO 10272-1:2006."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1111\/j.1469-0691.2007.01692.x","article-title":"A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory","volume":"13","author":"Persson","year":"2007","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1093\/infdis\/jir757","article-title":"Genomic characterization of enteroaggregative Escherichia coli from children in Mali","volume":"205","author":"Boisen","year":"2012","journal-title":"J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.mimet.2012.12.010","article-title":"A novel single-step multiplex polymerase chain reaction assay for the detection of diarrheagenic Escherichia coli","volume":"92","author":"Fujioka","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1128\/JCM.00860-12","article-title":"Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature","volume":"50","author":"Scheutz","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_28","unstructured":"EUCAST-The European Committee on Antimicrobial Susceptibility Testing (2022, October 04). Breakpoint tables for interpretation of MICs and zone diameters. Version 12.0., Available online: http:\/\/www.eucast.org."},{"key":"ref_29","unstructured":"Grimont, P.A.D., and Weill, F.X. (2007). Antigenic formulae of the Salmonella serovars, WHO Collaborating Centre for Reference and Research on Salmonella."},{"key":"ref_30","unstructured":"EUCAST-The European Committee on Antimicrobial Susceptibility Testing (2022, October 04). Comit\u00e9 de l\u2019antibiogramme de la Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie. Recommandations V.1.1 Avril., Available online: sfm-microbiologie.org."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_32","first-page":"1498E","article-title":"INNUENDO: A Cross-sectoral Platform for the Integration of Genomics in the Surveillance of Food-borne Pathogens","volume":"15","author":"Llarena","year":"2018","journal-title":"EFSA Support. Publ."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e102","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes De Novo Assembler","volume":"70","author":"Prjibelski","year":"2020","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11.7.1","DOI":"10.1002\/0471250953.bi1107s32","article-title":"Aligning Short Sequencing Reads with Bowtie","volume":"32","author":"Langmead","year":"2010","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Walker, B.J., Abeel, T., Shea, T., Priest, M., Abouelliel, A., Sakthikumar, S., Cuomo, C.A., Zeng, Q., Wortman, J., and Young, S.K. (2014). Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112963"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"R46","DOI":"10.1186\/gb-2014-15-3-r46","article-title":"Kraken: Ultrafast Metagenomic Sequence Classification Using Exact Alignments","volume":"15","author":"Wood","year":"2014","journal-title":"Genome Biol."},{"key":"ref_37","first-page":"e000166","article-title":"chewBBACA: A Complete Suite for Gene-by-Gene Schema Creation and Strain Identification","volume":"4","author":"Silva","year":"2018","journal-title":"Microb. Genom."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D660","DOI":"10.1093\/nar\/gkaa889","article-title":"Chewie Nomenclature Server (chewie-NS): A Deployable Nomenclature Server for Easy Sharing of Core and Whole Genome MLST Schemas","volume":"49","author":"Mamede","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mix\u00e3o, V., Pinto, M., Sobral, D., Di Pasquale, A., Gomes, J.P., and Borges, V. (2022). ReporTree: A Surveillance-Oriented Tool to Strengthen the Linkage between Pathogen Genetic Clusters and Epidemiological Data. Res. Sq.","DOI":"10.21203\/rs.3.rs-1404655\/v2"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1101\/gr.232397.117","article-title":"GrapeTree: Visualization of Core Genomic Relationships among 100,000 Bacterial Pathogens","volume":"28","author":"Zhou","year":"2018","journal-title":"Genome Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1089\/fpd.2012.1156","article-title":"Shiga toxin subtypes associated with Shiga toxin-producing Escherichia coli strains isolated from red deer, roe deer, chamois, and ibex","volume":"9","author":"Hofer","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1751-0147-48-7","article-title":"Campylobacter spp., Enterococcus spp., Escherichia coli, Salmonella spp., Yersinia spp., and Cryptosporidium oocysts in semi-domesticated reindeer (Rangifer tarandus tarandus) in Northern Finland and Norway","volume":"48","author":"Kemper","year":"2006","journal-title":"Acta Vet. Scand."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1751-0147-46-23","article-title":"Campylobacter spp., Salmonella spp., verocytotoxic Escherichia coli, and antibiotic resistance in indicator organisms in wild cervids","volume":"46","author":"Lillehaug","year":"2005","journal-title":"Acta Vet. Scand."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/AEM.01650-08","article-title":"Isolation and characterization of intestinal Escherichia coli clones from wild boars in Germany","volume":"75","author":"Schierack","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Cilia, G., Turchi, B., Fratini, F., Bilei, S., Boss\u00f9, T., De Marchis, M.L., Cerri, D., Pacini, M.I., and Bertelloni, F. (2021). Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy). Pathogens, 10.","DOI":"10.3390\/pathogens10020093"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13028-018-0395-3","article-title":"Distribution of enteropathogenic Yersinia spp. and Salmonella spp. in the Swedish wild boar population, and assessment of risk factors that may affect their prevalence","volume":"60","author":"Rosendal","year":"2018","journal-title":"Acta Vet. Scand."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.cimid.2012.11.004","article-title":"Prevalence and antimicrobial susceptibility of Salmonella in European wild boar (Sus scrofa); Latium Region-Italy","volume":"36","author":"Zottola","year":"2013","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1111\/tbed.13140","article-title":"Prevalence of Salmonella spp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates","volume":"66","author":"Molino","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Navarro-Gonzalez, N., Mentaberre, G., Porrero, C.M., Serrano, E., Mateos, A., L\u00f3pez-Mart\u00edn, J.M., Lav\u00edn, S., and Dom\u00ednguez, L. (2012). Effect of cattle on Salmonella carriage, diversity and antimicrobial resistance in free-ranging wild boar (Sus scrofa) in northeastern Spain. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0051614"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s10393-014-0921-3","article-title":"Campylobacter shared between free-ranging cattle and sympatric wild ungulates in a natural environment (NE Spain)","volume":"11","author":"Porrero","year":"2014","journal-title":"Ecohealth"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.cimid.2014.01.001","article-title":"Campylobacter infection in wild artiodactyl species from southern Spain: Occurrence, risk factors and antimicrobial susceptibility","volume":"37","author":"Carbonero","year":"2014","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_52","unstructured":"Antimicrobial Resistance Collaborators (2022). Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. Lancet, 399, 629\u2013655."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Collis, R.M., Biggs, P.J., Midwinter, A.C., Browne, A.S., Wilkinson, D.A., Irshad, H., French, N.P., Brightwell, G., and Cookson, A.L. (2020). Genomic epidemiology and carbon metabolism of Escherichia coli serogroup O145 reflect contrasting phylogenies. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0235066"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1017\/S0950268897007413","article-title":"Origin and characteristics of enteroinvasive strains of Escherichia coli (EIEC) isolated in Germany","volume":"118","author":"Beutin","year":"1997","journal-title":"Epidemiol. Infect."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.diagmicrobio.2015.07.016","article-title":"Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee","volume":"83","author":"Foster","year":"2015","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_56","unstructured":"(2022, October 04). STEC Infection: Annual Epidemiological Report for 2020. Available online: https:\/\/www.ecdc.europa.eu\/sites\/default\/files\/documents\/STEC-infection-AER-2020-JD-FINAL.pdf."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1046\/j.1462-2920.2001.00213.x","article-title":"The chicken, the egg and Salmonella Enteritidis","volume":"3","year":"2001","journal-title":"Environ. Microbiol."},{"key":"ref_58","unstructured":"ECDC and EFSA (European Centre for Disease Prevention and Control, European Food Safety Authority) (2022, October 04). Multi-Country Outbreak of Salmonella Enteritidis Sequence Type (ST)11 Infections Linked to Poultry Products in the EU\/EEA and the United Kingdom\u201325 February Stockholm: ECDC\/EFSA. Available online: https:\/\/efsa.onlinelibrary.wiley.com\/doi\/epdf\/10.2903\/sp.efsa.2021.EN-6486."},{"key":"ref_59","unstructured":"ECDC and EFSA (European Centre for Disease Prevention and Control, European Food Safety Authority) (2022, October 04). Multi-Country Outbreak of Salmonella Enteritidis Sequence Type (ST)11 Infections Linked to Eggs and Egg Products \u20138 February. Available online: https:\/\/www.ecdc.europa.eu\/sites\/default\/files\/documents\/ROA_Salmonella-Enteritidis-ST11_2022_final.pdf."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S1201-9712(01)90077-3","article-title":"Acute terminal ileitis mimicking Crohn\u2019s disease caused by Salmonella veneziana","volume":"5","author":"Dionisio","year":"2001","journal-title":"Int. J. Infect. Dis."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1128\/CMR.00006-15","article-title":"Global Epidemiology of Campylobacter Infection","volume":"28","author":"Kaakoush","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1038\/s41598-018-20889-x","article-title":"Updating the genomic taxonomy and epidemiology of Campylobacter hyointestinalis","volume":"8","author":"Wilkinson","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_63","unstructured":"(2022, October 04). The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-Borne Outbreaks in 2017. Available online: https:\/\/efsa.onlinelibrary.wiley.com\/doi\/epdf\/10.2903\/j.efsa.2018.5500."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1093\/cid\/cit055","article-title":"Enterohemorrhagic Escherichia coli O26:H11\/H: A new virulent clone emerges in Europe","volume":"56","author":"Bielaszewska","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"5411","DOI":"10.1128\/AEM.01728-13","article-title":"Detection of the emerging Shiga toxin-producing Escherichia coli O26:H11\/H- sequence type 29 (ST29) clone in human patients and healthy cattle in Switzerland","volume":"79","author":"Zweifel","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1053\/j.gastro.2008.12.072","article-title":"Intrahost genome alterations in enterohemorrhagic Escherichia coli","volume":"136","author":"Mellmann","year":"2009","journal-title":"Gastroenterology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"103352","DOI":"10.1016\/j.fm.2019.103352","article-title":"Shiga toxin-producing Escherichia coli isolates from red deer (Cervus elaphus), roe deer (Capreolus capreolus) and fallow deer (Dama dama) in Poland","volume":"86","author":"Siemionek","year":"2020","journal-title":"Food Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1128\/IAI.01182-10","article-title":"Shiga toxin subtypes display dramatic differences in potency","volume":"79","author":"Fuller","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1128\/JCM.03590-14","article-title":"Assessing the public health risk of Shiga toxin-producing Escherichia coli by use of a rapid diagnostic screening algorithm","volume":"53","author":"Ferdous","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.3389\/fmicb.2017.01471","article-title":"Characteristics of Shigatoxin-Producing Escherichia coli Strains Isolated during 2010\u20132014 from Human Infections in Switzerland","volume":"8","author":"Fierz","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s13213-011-0279-5","article-title":"Major virulence factors of enterotoxigenic Escherichia coli in pigs","volume":"62","author":"Duan","year":"2012","journal-title":"Ann. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.3201\/eid1509.090161","article-title":"Phylogeny and disease association of Shiga toxin-producing Escherichia coli O91","volume":"15","author":"Mellmann","year":"2009","journal-title":"Emerg. Infect. 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