{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:11:04Z","timestamp":1791274264776,"version":"4.1.0"},"reference-count":71,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T00:00:00Z","timestamp":1667001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Hydrogen sulfide (H2S) is a toxic and corrosive component that commonly occurs in biogas. In this study, H2S removal from swine-waste biogas using sulfur-oxidizing Paracoccus versutus CM1 immobilized in porous glass (PG) and polyurethane foam (PUF) biofilters was investigated. Bacterial compositions in the biofilters were also determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The biofilters were first tested on a laboratory scale under three space velocities (SV): 20, 30, and 40 h\u22121. Within 24 h, at an SV of 20 h\u22121, PG and PUF biofilters immobilized with P. versutus CM1 removed 99.5% and 99.7% of H2S, respectively, corresponding to the elimination capacities (EC) of 83.5 and 86.2 gm\u22123 h\u22121. On a pilot scale, with the horizontal PG-P. versutus CM1 biofilter operated at an SV of 30 h\u22121, a removal efficiency of 99.7% and a maximum EC of 113.7 gm\u22123 h\u22121 were achieved. No reduction in methane content in the outlet biogas was observed under these conditions. The PCR-DGGE analysis revealed that Paracoccus,\u00a0Acidithiobacillus, and Thiomonas were the predominant bacterial genera in the biofilters, which might play important roles in H2S removal. This PG\u2013P. versutus CM1 biofiltration system is highly efficient for H2S removal from swine-waste biogas.<\/jats:p>","DOI":"10.3390\/microorganisms10112148","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T09:01:42Z","timestamp":1667120502000},"page":"2148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Removal of Hydrogen Sulfide from Swine-Waste Biogas on a Pilot Scale Using Immobilized Paracoccus versutus CM1"],"prefix":"10.3390","volume":"10","author":[{"given":"Ladapa","family":"Kumdhitiahutsawakul","sequence":"first","affiliation":[{"name":"Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dolruedee","family":"Jirachaisakdeacha","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uthen","family":"Kantha","sequence":"additional","affiliation":[{"name":"Energy Research and Development Institute-Nakornping, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patiroop","family":"Pholchan","sequence":"additional","affiliation":[{"name":"Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1272-544X","authenticated-orcid":false,"given":"Pachara","family":"Sattayawat","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thararat","family":"Chitov","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Environmental Science Research Center (ESRC), Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0754-0638","authenticated-orcid":false,"given":"Yingmanee","family":"Tragoolpua","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Environmental Science Research Center (ESRC), Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakunnee","family":"Bovonsombut","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Environmental Science Research Center (ESRC), Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1007\/s13762-021-03301-6","article-title":"A critical review of biogas production and usage with legislations framework across the globe","volume":"19","author":"Abanades","year":"2022","journal-title":"Int. J. Sci. Environ. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11783-018-1037-8","article-title":"Biogas and its opportunities\u2014A review","volume":"12","author":"Kougias","year":"2018","journal-title":"Front. Environ. Sci. Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.renene.2018.03.006","article-title":"Biogas: Developments and perspectives in Europe","volume":"129","author":"Scarlat","year":"2018","journal-title":"Renew. Energy"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.enconman.2017.08.035","article-title":"Biogas as a renewable energy fuel\u2014A review of biogas upgrading, utilisation and storage","volume":"150","author":"Hashim","year":"2017","journal-title":"Energy Convers. Manag."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.rser.2016.01.117","article-title":"Potential of biogas production from farm animal waste in Malaysia","volume":"60","author":"Abdeshahian","year":"2016","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s13765-018-0390-4","article-title":"Potential of biogas production from swine manure in South Korea","volume":"61","author":"Lee","year":"2018","journal-title":"Appl. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s40093-016-0128-4","article-title":"Potential for biogas production from the anaerobic digestion of chicken droppings in Morocco","volume":"5","author":"Elasri","year":"2016","journal-title":"Int. J. Recycl. Org. Waste Agric."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pochwatka, P., Kowalczyk-Ju\u015bko, A., So\u0142owiej, P., Wawrzyniak, A., and Dach, J. (2020). Biogas plant exploitation in a middle-sized dairy farm in Poland: Energetic and economic aspects. Energies, 13.","DOI":"10.3390\/en13226058"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sherief, M., and Hassan, A.A. (2022). The Impact of wastewater quality and flow characteristics on H2S emissions generation: Statistical correlations and an artificial neural network model. Water, 14.","DOI":"10.3390\/w14050791"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.biteb.2018.01.006","article-title":"Use of sulfur-oxidizing bacteria enriched from sewage sludge to biologically remove H2S from biogas at an industrial-scale biogas plant","volume":"3","author":"Cheng","year":"2018","journal-title":"Bioresour. Technol. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"76","DOI":"10.5144\/0256-4947.59379","article-title":"A hydrogen sulfide exposure in an adult male","volume":"30","author":"Doujaiji","year":"2010","journal-title":"Ann. Saudi Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1177\/1091581810384882","article-title":"Hydrogen sulfide: Advances in understanding human toxicity","volume":"29","author":"Guidotti","year":"2010","journal-title":"Int. J. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2017.09.011","article-title":"Environmental toxicology of hydrogen sulfide","volume":"71","author":"Pearce","year":"2017","journal-title":"Nitric Oxide Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Choudhury, A., Shelford, T., Felton, G., Gooch, C., and Lansing, S. (2019). Evaluation of hydrogen sulfide scrubbing systems for anaerobic digesters on two U.S. Dairy farms. Energies, 12.","DOI":"10.3390\/en12244605"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"136388","DOI":"10.1016\/j.scitotenv.2019.136388","article-title":"Review on microaeration-based anaerobic digestion: State of the art, challenges, and prospectives","volume":"710","author":"Chen","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.psep.2018.06.014","article-title":"On-farm trials of practical options for hydrogen sulphide removal from piggery biogas","volume":"117","author":"Skerman","year":"2018","journal-title":"Process Saf. Environ. Prot."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.biortech.2012.12.114","article-title":"Oxidation of hydrogen sulfide in biogas using dissolved oxygen in the extreme acidic biofiltration operation","volume":"131","author":"Charnnok","year":"2013","journal-title":"Bioresour. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.rser.2012.08.009","article-title":"Transforming biogas into biomethane using membrane technology","volume":"17","author":"Scholz","year":"2013","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"142753","DOI":"10.1016\/j.scitotenv.2020.142753","article-title":"Biomethane production from anaerobic co-digestion at wastewater treatment plants: A critical review on development and innovations in biogas upgrading techniques","volume":"765","author":"Nguyen","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_20","first-page":"2","article-title":"Removal of hydrogen sulfide from gas streams using biological processes\u2014A review","volume":"48","author":"Syed","year":"2006","journal-title":"Can. Biosyst. Eng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1016\/j.scitotenv.2008.09.054","article-title":"Application of bacteria involved in the biological sulfur cycle for paper mill effluent purification","volume":"407","author":"Janssen","year":"2009","journal-title":"Sci. Total Environ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3485","DOI":"10.1007\/s11270-012-1126-4","article-title":"Simultaneous removal of H2S, NH3, and ethyl mercaptan in biotrickling filters packed with poplar wood and polyurethane foam: Impact of pH during startup and crossed effects evaluation","volume":"223","author":"Lafuente","year":"2012","journal-title":"Water Air Soil Pollut."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s10098-013-0594-6","article-title":"Management and control of air emissions from electronic industries","volume":"16","author":"Babar","year":"2014","journal-title":"Clean Technol. Environ. Policy"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s11157-015-9379-1","article-title":"A review on the state-of-the-art of physical\/chemical and biological technologies for biogas upgrading","volume":"14","author":"Meier","year":"2015","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jenvman.2014.09.004","article-title":"Field evaluation of wood bark-based down-flow biofilters for mitigation of odor, ammonia, and hydrogen sulfide emissions from confined swine nursery barns","volume":"147","author":"Kafle","year":"2015","journal-title":"J. Environ. Manag."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.bej.2018.10.014","article-title":"Treatment of malodorous air in biotrickling filters: A review","volume":"141","author":"Rybarczyk","year":"2019","journal-title":"Biochem. Eng. J."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Le Borgne, S., and Baquerizo, G. (2019). Microbial ecology of biofiltration units used for the desulfurization of biogas. Chem. Eng., 3.","DOI":"10.3390\/chemengineering3030072"},{"key":"ref_28","first-page":"9","article-title":"Removal of hydrogen sulfide gas using biofiltration\u2014A review","volume":"9","author":"Rattanapan","year":"2011","journal-title":"Walailak J. Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1002\/elsc.202100006","article-title":"Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur-oxidizing bacteria","volume":"21","author":"Chen","year":"2021","journal-title":"Eng. Life Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s10098-007-0110-y","article-title":"Biofiltration of wastewater lift station emissions: Evaluation of VOC removal in the presence of H2S","volume":"10","author":"Martinez","year":"2008","journal-title":"Clean Technol. Environ. Policy"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.biotechadv.2015.02.007","article-title":"Sulfur-oxidizing bacteria in environmental technology","volume":"33","author":"Pokorna","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1099\/13500872-141-6-1469","article-title":"Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus","volume":"141","author":"Katayama","year":"1995","journal-title":"Microbiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/j.egypro.2015.11.582","article-title":"Comparative performance of Halothiobacillus Neapolitanus and Paracoccus Pantotrophus in sulphur oxidation","volume":"79","author":"Vikromvarasiri","year":"2015","journal-title":"Energy Procedia"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.biortech.2018.03.123","article-title":"Paracoccus versutus KS293 adaptation to aerobic and anaerobic denitrification: Insights from nitrogen removal, functional gene abundance, and proteomic profiling analysis","volume":"260","author":"Zhang","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"119351","DOI":"10.1016\/j.jclepro.2019.119351","article-title":"Resilient performance of an anoxic biotrickling filter for hydrogen sulphide removal from a biogas mimic: Steady, transient state and neural network evaluation","volume":"249","author":"Watsuntorn","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_36","first-page":"872","article-title":"Hydrogen sulfide removal from biogas using immobilized sulfur oxidizing bacterium Paracoccus versutus CM1 in biofilters","volume":"47","author":"Jirachaisakdeacha","year":"2020","journal-title":"Chiang Mai J. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jenvman.2019.04.003","article-title":"Technical and economic investigation of chemical scrubber and bio-filtration in removal of H2S and NH3 from wastewater treatment plant","volume":"241","author":"Alinezhad","year":"2019","journal-title":"J. Environ. Manag."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1007\/s10098-015-1043-5","article-title":"Biofiltration of H2S-rich biogas using Acidithiobacillus thiooxidans","volume":"18","author":"Aita","year":"2016","journal-title":"Clean Technol. Environ. Policy"},{"key":"ref_39","first-page":"121","article-title":"Biological removal of H2S gas in a semi-pilot scale biotrickling filter: Optimization of various parameters for efficient removal at high loading rates and low pH conditions","volume":"285","author":"Bae","year":"2019","journal-title":"Bioresour. Technol."},{"key":"ref_40","first-page":"444","article-title":"Use of the PCR-DGGE technique to determine the microbial community in anaerobic activated sludges from biogas plants","volume":"46","author":"Kumdhitiahutsawakul","year":"2019","journal-title":"Chiang Mai J. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/BF02529967","article-title":"Phylogenetic-relationships-ofThiomicrospira-species-and-their-identification-in-deep-sea-hydrothermal-vent-samples-by-denaturing-gradient-gel-electrophoresis","volume":"164","author":"Muyzer","year":"1995","journal-title":"Arch. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"126127","DOI":"10.1016\/j.chemosphere.2020.126127","article-title":"The performance and microbial community in a slightly alkaline biotrickling filter for the removal of high concentration H2S from biogas","volume":"249","author":"Wu","year":"2020","journal-title":"Chemosphere"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1016\/j.renene.2019.07.047","article-title":"A comprehensive review on recent biological innovations to improve biogas production, Part 2: Mainstream and downstream strategies","volume":"146","author":"Tabatabaei","year":"2020","journal-title":"Renew. Energy"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1007\/s00253-008-1759-9","article-title":"Transient-state biodegradation behavior of a horizontal biotrickling filter in co-treating gaseous H2S and NH3","volume":"81","author":"Jiang","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.biortech.2006.12.028","article-title":"Biological oxidation of hydrogen sulfide under steady and transient state conditions in an immobilized cell biofilter","volume":"99","author":"Kim","year":"2008","journal-title":"Bioresour. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/j.chemosphere.2013.05.054","article-title":"Elimination of high concentration hydrogen sulfide and biogas purification by chemical-biological process","volume":"92","author":"Ho","year":"2013","journal-title":"Chemosphere"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bej.2013.12.019","article-title":"Hydrogen sulfide removal from air by biotrickling filter using open-pore polyurethane foam as a carrier","volume":"84","author":"Solcia","year":"2014","journal-title":"Biochem. Eng. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jenvman.2015.09.008","article-title":"The performance of a two-layer biotrickling filter filled with new mixed packing materials for the removal of H2S from air","volume":"165","author":"Chen","year":"2016","journal-title":"J. Environ. Manag."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"741","DOI":"10.18331\/BRJ2017.4.4.6","article-title":"A mini review on prospects and challenges of biological H2S removal from biogas with focus on biotrickling filtration and microaerobic desulfurization A review on prospects and challenges of biological H2S removal from biogas with focus on biotrickling filtration and microaerobic desulfurization","volume":"16","author":"Khoshnevisan","year":"2017","journal-title":"Biofuel.Res. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jhazmat.2019.05.043","article-title":"Transient\u2013state operation of an anoxic biotrickling filter for H2S removal","volume":"377","author":"Khanongnuch","year":"2019","journal-title":"J. Hazard. Mater."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.2166\/wpt.2022.072","article-title":"Removal of hydrogen sulfide in biogas from wastewater treatment sludge by real-scale biotrickling filtration desulfurization process","volume":"17","author":"Koyuncu","year":"2022","journal-title":"Water Pract. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"29554","DOI":"10.1016\/j.ijhydene.2019.03.069","article-title":"Hydrogen sulfide removal from biogas in biotrickling filter system inoculated with Paracoccus pantotrophus","volume":"44","author":"Juntranapaporn","year":"2019","journal-title":"Int. J. Hydrog. Energy."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Tayar, S.P., Guerrero, R.d.B.S., Hidalgo, L.F., and Bevilaqua, D. (2019). Evaluation of biogas bio-desulfurization using different packing materials. ChemEngineering, 3.","DOI":"10.3390\/chemengineering3010027"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"27820","DOI":"10.1016\/j.ijhydene.2017.05.232","article-title":"Performance of Paracoccus pantotrophus for H2S removal in biotrickling filter","volume":"42","author":"Vikromvarasiri","year":"2017","journal-title":"Int. J. Hydrog. Energy"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5617","DOI":"10.1016\/j.jece.2017.10.029","article-title":"Hydrogen sulfide removal from a biogas mimic by biofiltration under anoxic conditions","volume":"5","author":"Jaber","year":"2017","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1007\/s00253-011-3191-9","article-title":"Integrating microbial ecology in bioprocess understanding: The case of gas biofiltration","volume":"90","author":"Cabrol","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_58","first-page":"162645","article-title":"Use of PCR-DGGE based molecular methods to analyse microbial community diversity and stability during the thermophilic stages of an ATAD wastewater sludge treatment process as an aid to performance monitoring","volume":"2013","author":"Piterina","year":"2013","journal-title":"Int. Sch. Res. Not."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1016\/j.meatsci.2013.09.013","article-title":"Effective survival of immobilized Lactobacillus casei during ripening and heat treatment of probiotic dry-fermented sausages and investigation of the microbial dynamics","volume":"96","author":"Sidira","year":"2014","journal-title":"Meat Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2416","DOI":"10.3389\/fmicb.2018.02416","article-title":"Dynamic and functional characteristics of predominant species in industrial paocai as revealed by combined DGGE and metagenomic sequencing","volume":"9","author":"Liang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"331","DOI":"10.26656\/fr.2017.5(2).526","article-title":"Microbial and metabolite profiles of spontaneous and adjunct-inoculated cacao (Theobroma cacao L.) fermentation","volume":"5","author":"Peralta","year":"2021","journal-title":"Food Res."},{"key":"ref_62","first-page":"1","article-title":"Investigation of biogas for enhancing oil recovery with indigenous microorganism in high salinity oil field","volume":"40","author":"Wang","year":"2022","journal-title":"Pet. Sci. Technol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1263\/jbb.101.309","article-title":"Removal of hydrogen sulfide by sulfate-resistant Acidithiobacillus thiooxidans AZ11","volume":"101","author":"Lee","year":"2006","journal-title":"J. Biosci. Bioeng."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Yang, Z., Liu, Z., Sklodowska, A., Musialowski, M., Bajda, T., Yin, H., and Drewniak, L. (2021). Microbiological sulfide removal\u2014From microorganism isolation to treatment of industrial effluent. Microorganisms, 9.","DOI":"10.3390\/microorganisms9030611"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.jhazmat.2012.06.029","article-title":"Removal of nitrate using Paracoccus sp. YF1 immobilized on bamboo carbon","volume":"229","author":"Liu","year":"2012","journal-title":"J. Hazard. Mater."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.biortech.2013.08.052","article-title":"Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM","volume":"148","author":"Shi","year":"2013","journal-title":"Bioresour. Technol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"17870","DOI":"10.1038\/s41598-019-54281-0","article-title":"Variations in microbial community structure and functional gene expression in bio-treatment processes with odorous pollutants","volume":"9","author":"Li","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.chemosphere.2010.05.019","article-title":"Bacterial community analysis of a gas-phase biotrickling filter for biogas mimics desulfurization through the rRNA approach","volume":"8","author":"Maestre","year":"2010","journal-title":"Chemosphere"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1007\/s12010-015-1545-y","article-title":"The removal of hydrogen sulfide from biogas in a microaerobic botrickling filter using polypropylene carrier as packing material","volume":"175","author":"Zhou","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_70","unstructured":"Haosagul, S. (2019). Biochip for Monitoring Microbial Community in Industrial Scale Hydrogen Sulfide Removal System. [Ph.D. Thesis, King Mongkut\u2019s University of Technology Thonburi]. 1 Semester."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1002\/bit.20443","article-title":"Operational and microbiological aspects of a bioaugmented two-stage biotrickling filter removing hydrogen sulfide and dimethyl sulfide","volume":"90","author":"Sercu","year":"2005","journal-title":"Biotechnol. Bioeng."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2148\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:05:50Z","timestamp":1760144750000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,29]]},"references-count":71,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["microorganisms10112148"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10112148","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,29]]}}}