{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:22Z","timestamp":1787028982450,"version":"build-2736575974"},"reference-count":33,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T00:00:00Z","timestamp":1668211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Florida\u2014Department of Small Animal Clinical Sciences Intramural Grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Coagulase positive Staphylococci (CoPS) are the leading cause of canine cutaneous and otic infections. Virulence factors associated with Staphylococci include the expression of mec and panton-valentine leukocidin (pvl) genes. Methicillin-resistance (MR) is commonly associated with mecA gene expression, although a recently identified variant, mecC, has been reported. This study aims to evaluate the prevalence of mecA, mecC and pvl genes in 232 clinical isolates of CoPS collected from dogs with pyoderma. A multiplex PCR, and Kirby-Bauer disk diffusion susceptibility test for cefoxitin was performed for all isolates. PBP2a agglutination test was performed on 127 isolates. Standard MRSA isolates were used as positive controls. The mecA gene was identified in 149\/232 isolates (64.2%): 116 S. pseudintermedius, 30 S. coagulans and three S. aureus. The pvl gene was present in only 1 isolate of S. pseudintermedius (0.4%), whereas no isolates carried the mecC gene. 34 isolates were resistant to cefoxitin (14.6%) and they were all mecA positive. The results of this study show an MR prevalence of 64.2% confirming concerns about antibiotic resistance in veterinary medicine. In conclusion, this is the first study analyzing the prevalence of mecC and pvl in comparison to mecA, in a large cohort of CoPS clinical isolates from dogs with pyoderma. A multimodal surveillance on the prevalence of mecC and pvl in veterinary medicine is essential to appropriate antimicrobial management.<\/jats:p>","DOI":"10.3390\/microorganisms10112239","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T02:34:58Z","timestamp":1668393298000},"page":"2239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Prevalence of mecA, mecC and Panton-Valentine-Leukocidin Genes in Clinical Isolates of Coagulase Positive Staphylococci from Dermatological Canine Patients"],"prefix":"10.3390","volume":"10","author":[{"given":"Marcela O.","family":"Platenik","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Archer","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lopamudra","family":"Kher","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4893-4289","authenticated-orcid":false,"given":"Domenico","family":"Santoro","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1111\/j.1365-3164.2006.00536.x","article-title":"Screening of Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus schleiferi isolates obtained from small companion animals for antimicrobial resistance: A retrospective review of isolates (2003\u201304)","volume":"17","author":"Morris","year":"2006","journal-title":"Vet. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1093\/jac\/dkr367","article-title":"Review on methicillin-resistant Staphylococcus pseudintermedius","volume":"66","author":"Catry","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1080\/01652176.2017.1400710","article-title":"Molecular characteristics of new clonal complexes of Staphylococcus pseudintermedius from clinically normal dogs","volume":"38","author":"Han","year":"2018","journal-title":"Vet. Q."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5926","DOI":"10.1099\/ijsem.0.004498","article-title":"Phylogenomic analyses of the Staphylococcaceae family suggest the reclassification of five species within the genus Staphylococcus as heterotypic synonyms, the promotion of five subspecies to novel species, the taxonomic reassignment of five Staphylococcus species to Mammaliicoccus gen. nov., and the formal assignment of Nosocomiicoccus to the family Staphylococcaceae","volume":"70","author":"Madhaiyan","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1002\/vms3.701","article-title":"Prevalence of coagulase-positive methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in dogs in Bangladesh","volume":"8","author":"Rana","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"001407","DOI":"10.1099\/jmm.0.001407","article-title":"Genomic epidemiology of the opportunistic pathogen Staphylococcus coagulans from companion dogs","volume":"70","author":"Paterson","year":"2021","journal-title":"J. Med. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1128\/JCM.01336-06","article-title":"Comparison of tests to detect oxacillin resistance in Staphylococcus intermedius, Staphylococcus schleiferi, and Staphylococcus aureus isolates from canine hosts","volume":"44","author":"Bemis","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4093","DOI":"10.1128\/IAI.00542-15","article-title":"Fibronectin Binding Proteins SpsD and SpsL Both Support Invasion of Canine Epithelial Cells by Staphylococcus pseudintermedius","volume":"83","author":"Pietrocola","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Velasco, V., Sherwood, J.S., Rojas-Garc\u00eda, P.P., and Logue, C.M. (2014). Multiplex Real-Time PCR for Detection of Staphylococcus aureus, mecA and Panton-Valentine Leukocidin (PVL) Genes from Selective Enrichments from Animals and Retail Meat. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0097617"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.1128\/JCM.43.10.5026-5033.2005","article-title":"Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus","volume":"43","author":"Zhang","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"E16","DOI":"10.1111\/1469-0691.12036","article-title":"Epidemiology of methicillin-resistant Staphylococcus aureus carrying the novel mecC gene in Denmark corroborates a zoonotic reservoir with transmission to humans","volume":"19","author":"Petersen","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1111\/1348-0421.12059","article-title":"Characterization of methicillin- resistant Staphylococcus pseudintermedius isolated from dogs and cats","volume":"57","author":"Bardiau","year":"2013","journal-title":"Microbiol. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetmic.2019.02.014","article-title":"Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals","volume":"230","author":"Loncaric","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.tim.2013.11.003","article-title":"The emergence of mecC methicillin-resistant Staphylococcus aureus","volume":"22","author":"Paterson","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1093\/jac\/dkt462","article-title":"Prevalence and characterization of human mecC methicillin-resistant Staphylococcus aureus isolates in England","volume":"69","author":"Paterson","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1093\/jac\/48.suppl_1.65","article-title":"Detection of methicillin\/oxacillin resistance in staphylococci","volume":"48","author":"Brown","year":"2001","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4518541","DOI":"10.1155\/2018\/4518541","article-title":"Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study","volume":"2018","author":"Karmakar","year":"2018","journal-title":"J. Pathog."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1111\/j.1469-0691.2004.00976.x","article-title":"Detection of Panton\u2013Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: Clinical and epidemiological aspects","volume":"10","author":"Johnsson","year":"2004","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/JCM.02864-15","article-title":"Evaluation of Oxacillin and Cefoxitin Disk and MIC Breakpoints for Prediction of Methicillin Resistance in Human and Veterinary Isolates of Staphylococcus intermedius Group","volume":"54","author":"Wu","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1128\/JCM.00075-13","article-title":"Evaluation of a Modular Multiplex-PCR Methicillin-Resistant Staphylococcus aureus Detection Assay Adapted for mecC Detection","volume":"51","author":"Becker","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","unstructured":"CLSI (2010). Surveillance for Methicillin-Resistant Staphylococcus aureus: Principles, Practices, and Challenges, A Report., Clinical and Laboratory Standards Institute. CLSI Docu ment X07-R."},{"key":"ref_23","unstructured":"CLSI (2015). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolates form Animals, Clinical and Laboratory Standards Institute. [3rd ed.]. CLSI Supplement VET01S."},{"key":"ref_24","unstructured":"CLSI (2015). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. 25th Informational Supplement. M100-S25."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1128\/JCM.02193-06","article-title":"Methicillin-Resistant Staphylococcus pseudintermedius in a Veterinary Teaching Hospital","volume":"45","author":"Sasaki","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","unstructured":"(2022, February 02). GENEWIZ, USA. Available online: https:\/\/www.genewiz.com\/en\/Public\/Services\/Next-Generation-Sequencing\/Standalone-NGS-Solutions."},{"key":"ref_27","unstructured":"(2022, February 02). QIAGEN Multiplex PCR Kit Handbook. Available online: https:\/\/www.qiagen.com\/us\/resources\/download.aspx?id=a541a49c-cd06-40ca-b1d2-563d0324ad6c&lang=en."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1292\/jvms.10-0172","article-title":"Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans Isolated from Dogs with Pyoderma in Japan","volume":"72","author":"Kawakami","year":"2010","journal-title":"J. Vet. Med. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1128\/JCM.38.6.2051-2054.2000","article-title":"Rapid detection of mecA-positive and mecA-negative Staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test","volume":"38","author":"Hussain","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1128\/MMBR.00052-12","article-title":"Role of Pore-Forming Toxins in Bacterial Infectious Diseases","volume":"77","author":"Los","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.vetmic.2013.04.011","article-title":"Staphylococcus pseudintermedius heterogeneously expresses the mecA gene","volume":"165","author":"Savini","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vetmic.2016.08.009","article-title":"Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC","volume":"200","author":"Seidel","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"376","DOI":"10.3389\/fvets.2020.00376","article-title":"Molecular Detection and Characterization of the mecA and nuc Genes From Staphylococcus Species (S. aureus, S. pseudintermedius, and S. schleiferi) Isolated From Dogs Suffering Superficial Pyoderma and Their Antimicrobial Resistance Profiles","volume":"7","author":"Carvajal","year":"2020","journal-title":"Front. Vet. Sci."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2239\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:16:57Z","timestamp":1760145417000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,12]]},"references-count":33,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["microorganisms10112239"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10112239","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,12]]}}}