{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T12:01:47Z","timestamp":1776859307535,"version":"3.51.2"},"reference-count":33,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Disease","award":["(ZIAAI001331)"],"award-info":[{"award-number":["(ZIAAI001331)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Coxiella burnetii is a Gram-negative, intracellular bacterium that causes the zoonosis Q fever. Among the many natural isolates of C. burnetii recovered from various sources, the Dugway group exhibits unique genetic characteristics, including the largest C. burnetii genomes. These strains were isolated during 1954\u20131958 from wild rodents from the Utah, USA desert. Despite retaining phase I lipopolysaccharide and the type 4B secretion system, two critical virulence factors, avirulence has been reported in a guinea pig infection model. Using guinea pig models, we evaluated the virulence, whole-cell vaccine (WCV) efficacy, and post-vaccination hypersensitivity (PVH) potential of a representative Dugway strain. Consistent with prior reports, Dugway appeared to be highly attenuated compared to a virulent strain. Indeed, Dugway-infected animals showed similarly low levels of fever, body weight loss, and splenomegaly like Nine Mile II-infected animals. When compared to a human Q fever vaccine, QVax\u00ae, Dugway WCV exhibited analogous protection against a heterologous Nine Mile I challenge. PVH was investigated in a skin-testing model which revealed significantly decreased maximum erythema in Dugway \u0394dot\/icm WCV-skin-tested animals compared to that of QVax\u00ae. These data provide insight into this unique bacterial strain and implicate its potential use as a mutated WCV candidate.<\/jats:p>","DOI":"10.3390\/microorganisms10112261","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T02:28:58Z","timestamp":1668479338000},"page":"2261","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of Coxiella burnetii Dugway Strain Host-Pathogen Interactions In Vivo"],"prefix":"10.3390","volume":"10","author":[{"given":"Mahelat","family":"Tesfamariam","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Picabo","family":"Binette","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane","family":"Cockrell","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Beare","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Heinzen","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl","family":"Shaia","sequence":"additional","affiliation":[{"name":"Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5030-975X","authenticated-orcid":false,"given":"Carrie Mae","family":"Long","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"ref_1","first-page":"829","article-title":"The geographical distribution of Q fever","volume":"13","author":"Kaplan","year":"1955","journal-title":"Bull World Health Organ"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wardrop, N.A., Thomas, L.F., Cook, E.A.J., De Glanville, W.A., Atkinson, P.M., Wamae, C.N., and F\u00e8vre, E.M. 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