{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:45:25Z","timestamp":1791434725077,"version":"4.3.3"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T00:00:00Z","timestamp":1668643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science (JSPS)","award":["18K17252"],"award-info":[{"award-number":["18K17252"]}]},{"name":"Japan Society for the Promotion of Science (JSPS)","award":["21K10184"],"award-info":[{"award-number":["21K10184"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Immune thrombocytopenic purpura (ITP) is an autoimmune disease characterized by isolated cryptogenic thrombocytopenia due to a transient or persistent reduction in platelet count. Many patients with ITP have shown improved platelet count after Helicobacter pylori eradication therapy. However, there have been no studies regarding H. pylori in the oral cavity of patients with ITP. Here, we describe a patient with ITP whose oral samples exhibited H. pylori. A 64-year-old woman with ITP came to our hospital with chief complaints that required oral surgery, including tooth extraction and cystectomy. Bacterial DNA from H. pylori was confirmed on the extracted tooth, but was not detected in the saliva taken at the time. Bacterial DNA from H. pylori was detected on the suture around the extraction socket, which was removed at 10 days post-operation. However, H. pylori DNA was not detected in other oral samples at 10 or 30 days post-operation. A urea breath test was carried out in the gastrointestinal clinic at 60 days post-operation, which revealed no presence of H. pylori in the gastrointestinal tract. These results suggest that teeth with severe bacterial infections may be a potential reservoir of H. pylori for patients with ITP.<\/jats:p>","DOI":"10.3390\/microorganisms10112285","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T03:35:23Z","timestamp":1668742523000},"page":"2285","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of Helicobacter pylori from Extracted Teeth of a Patient with Idiopathic Thrombocytopenic Purpura"],"prefix":"10.3390","volume":"10","author":[{"given":"Masakazu","family":"Hamada","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery II, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryota","family":"Nomura","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"},{"name":"Department of Pediatric Dentistry, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima 734-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saaya","family":"Matayoshi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Ogaya","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8007-3274","authenticated-orcid":false,"given":"Hiroyasu","family":"Kameyama","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery II, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narikazu","family":"Uzawa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery II, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1182\/blood-2008-07-162503","article-title":"Standardization of terminology, definitions and outcome criteria in immune thrombocytopenic purpura of adults and children: Report from an international working group","volume":"113","author":"Rodeghiero","year":"2009","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3389\/fimmu.2018.00880","article-title":"Emerging concepts in immune thrombocytopenia","volume":"9","author":"Swinkels","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"410","DOI":"10.4103\/0976-237X.137976","article-title":"Idiopathic thrombocytopenic purpura","volume":"5","author":"Kayal","year":"2014","journal-title":"Contemp. Clin. Dent."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1136\/adc.71.3.251","article-title":"Intracranial haemorrhage in idiopathic thrombocytopenic purpura. Paediatric Haematology Forum of the British Society for Haematology","volume":"71","author":"Lilleyman","year":"1994","journal-title":"Arch. Dis. Child."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1097\/00043426-200308000-00017","article-title":"Intracranial hemorrhage in immune thrombocytopenic purpura: A retrospective analysis","volume":"25","author":"Butros","year":"2003","journal-title":"J. Pediatr. Hematol. Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4777","DOI":"10.1182\/blood-2009-04-215525","article-title":"Intracranial hemorrhage (ICH) in children with immune thrombocytopenia (ITP): Study of 40 cases","volume":"114","author":"Psaila","year":"2009","journal-title":"Blood"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"164","DOI":"10.2174\/1874210601408010164","article-title":"Immune thrombocytopenic purpura presenting as unprovoked gingival hemorrhage: A case report","volume":"8","author":"Bal","year":"2014","journal-title":"Open Dent. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S147","DOI":"10.1016\/0090-1229(89)90080-9","article-title":"The use of intravenous gamma-globulin in idiopathic thrombocytopenic purpura","volume":"53","author":"Bussel","year":"1989","journal-title":"Clin. Immunol. Immunopathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1001\/archinte.1994.00420070021003","article-title":"Helicobacter pylori","volume":"154","author":"Fennerty","year":"1994","journal-title":"Arch. Intern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"11221","DOI":"10.3748\/wjg.v21.i40.11221","article-title":"Diagnosis of Helicobacter pylori infection: Current options and developments","volume":"21","author":"Wang","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e12411","DOI":"10.1111\/hel.12411","article-title":"Helicobacter pylori infection and extragastric diseases in 2017","volume":"22","author":"Ianiro","year":"2017","journal-title":"Helicobacter"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/hel.12340","article-title":"Helicobacter pylori and extragastric diseases","volume":"21","author":"Goni","year":"2016","journal-title":"Helicobacter"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/S0140-6736(05)60004-9","article-title":"Regression of autoimmune thrombocytopenia after eradication of Helicobacter pylori","volume":"352","author":"Gasbarrini","year":"1998","journal-title":"Lancet"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"356","DOI":"10.5009\/gnl14483","article-title":"The effects of Helicobacter pylori eradication therapy for chronic idiopathic thrombocytopenic purpura","volume":"10","author":"Hwang","year":"2016","journal-title":"Gut. Liver"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.5539\/gjhs.v8n7p35","article-title":"Impact of Helicobacter pylori eradication therapy on platelet counts in patients with chronic idiopathic thrombocytopenic purpura","volume":"8","author":"Amiri","year":"2015","journal-title":"Glob. J. Health Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1182\/asheducation-2008.1.206","article-title":"Helicobacter pylori and chronic ITP","volume":"2008","author":"Stasi","year":"2008","journal-title":"Hematol. Am. Soc. Hematol. Educ. Program"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.5504\/BBEQ.2011.0078","article-title":"Oral cavity and systemic diseases\u2014Helicobacter pylori and dentistry","volume":"25","author":"Krasteva","year":"2011","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_18","first-page":"215","article-title":"Diagnosis of Helicobacter pylori infection","volume":"241","author":"Westblom","year":"1999","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1590\/S0074-02762010000500009","article-title":"Helicobacter pylori transiently in the mouth may participate in the transmission of infection","volume":"105","author":"Silva","year":"2010","journal-title":"Mem. Inst. Oswaldo. Cruz."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1099\/jmm.0.079491-0","article-title":"Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents","volume":"64","author":"Ogaya","year":"2015","journal-title":"J. Med. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Nomura, R., Ogaya, Y., Matayoshi, S., Morita, Y., and Nakano, K. (2018). Molecular and clinical analyses of Helicobacter pylori colonization in inflamed dental pulp. BMC Oral Health, 18.","DOI":"10.1186\/s12903-018-0526-2"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1136\/jcp.46.6.540","article-title":"Identification of Helicobacter pylori DNA in the mouths and stomachs of patients with gastritis using PCR","volume":"46","author":"Mapstone","year":"1993","journal-title":"J. Clin. Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1046\/j.1523-5378.2000.00004.x","article-title":"Influence of oral Helicobacter pylori on the success of eradication therapy against gastric Helicobacter pylori","volume":"5","author":"Miyabayashi","year":"2000","journal-title":"Helicobacter"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1023\/A:1016417200611","article-title":"Comparison of cytotoxin genotypes of Helicobacter pylori in stomach and saliva","volume":"47","author":"Wang","year":"2002","journal-title":"Dig. Dis. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1128\/JCM.41.7.3336-3338.2003","article-title":"Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures","volume":"41","author":"Park","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.3748\/wjg.v10.i13.1958","article-title":"Comparison of three PCR methods for detection of Helicobacter pylori DNA and detection of cagA gene in gastric biopsy specimens","volume":"10","author":"Smith","year":"2004","journal-title":"World J. Gastroenterol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1128\/JCM.00377-06","article-title":"Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens","volume":"44","author":"Nakano","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0140-6736(96)07023-7","article-title":"Helicobacter pylori infection","volume":"349","author":"Goodwin","year":"1997","journal-title":"Lancet"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4845","DOI":"10.1038\/s41598-019-41166-5","article-title":"Potential involvement of Helicobacter pylori from oral specimens in overweight body-mass index","volume":"9","author":"Hamada","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s10266-018-0393-y","article-title":"Comparison of oral flora before and after triple therapy for Helicobacter pylori eradication in patient with gastric disease","volume":"107","author":"Kadota","year":"2019","journal-title":"Odontology"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Iwai, K., Watanabe, I., Yamamoto, T., Kuriyama, N., Matsui, D., Nomura, R., Ogaya, Y., Oseko, F., Adachi, K., and Takizawa, S. (2019). Association between Helicobacter pylori infection and dental pulp reservoirs in Japanese adults. BMC Oral. Health, 19.","DOI":"10.1186\/s12903-019-0967-2"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"14150","DOI":"10.1038\/s41598-022-18409-z","article-title":"Characterization of the unique oral microbiome of children with Down syndrome","volume":"12","author":"Mitsuhata","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"208","DOI":"10.2334\/josnusd.21-0464","article-title":"Three-year survey of oral hygiene conditions of Cambodian public primary school children","volume":"64","author":"Asao","year":"2022","journal-title":"J. Oral Sci."},{"key":"ref_34","unstructured":"Asao, Y., Iwamoto, Y., Chea, C., Chher, T., Mitsuhata, C., Naito, M., and Kozai, K. (2022). The effect of improving oral health literacy among teachers on the oral health condition of primary schoolchildren in Cambodia. Eur. J. Paediatr. Dent., in press."},{"key":"ref_35","first-page":"123","article-title":"Periodontal pockets as a reservoir of Helicobacter pylori causing relapse of gastric ulcer: A review of the literature","volume":"29","author":"Lauritano","year":"2015","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1177\/0300060518816158","article-title":"Effects of periodontal therapy on eradication and recurrence of Helicobacter pylori infection after successful treatment","volume":"47","author":"Tongtawee","year":"2019","journal-title":"J. Int. Med. Res."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kadota, T., Hamada, M., Nomura, R., Ogaya, Y., Okawa, R., Uzawa, N., and Nakano, K. (2020). Distribution of Helicobacter pylori and periodontopathic bacterial species in the oral cavity. Biomedicines, 8.","DOI":"10.3390\/biomedicines8060161"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"126","DOI":"10.4292\/wjgpt.v7.i1.126","article-title":"Helicobacter pylori and its reservoirs: A correlation with the gastric infection","volume":"7","author":"Rasmussen","year":"2016","journal-title":"World. J. Gastrointest. Pharmacol. Ther."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2285\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:20:19Z","timestamp":1760145619000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,17]]},"references-count":38,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["microorganisms10112285"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10112285","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,17]]}}}