{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:20:39Z","timestamp":1762075239903,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T00:00:00Z","timestamp":1668816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010751","name":"FONDECYT","doi-asserted-by":"publisher","award":["1180265","5392202MC","1191902"],"award-info":[{"award-number":["1180265","5392202MC","1191902"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DICYT","award":["1180265","5392202MC","1191902"],"award-info":[{"award-number":["1180265","5392202MC","1191902"]}]},{"DOI":"10.13039\/501100010751","name":"FONDECYT","doi-asserted-by":"publisher","award":["1180265","5392202MC","1191902"],"award-info":[{"award-number":["1180265","5392202MC","1191902"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Previously, we reported an in vitro evaluation regarding antibacterial effects against F. psychrophilum by a new Cu (I) complex, [Cu(NN1)2](ClO4). This study presents the results of an in vivo evaluation of [Cu(NN1)2](ClO4) added as a dietary supplement against F. psychrophilum in rainbow trout. The results showed that the administration of [Cu(NN1)2](ClO4) at 29 and 58 \u00b5g\/g of fish for 15 days does not affect the growth of rainbow trout. On the other hand, the amount of copper present in the liver, intestine, and muscle of rainbow trout was determined. The results showed that the amount of copper in the liver, when compared between treated fish and control fish, does not change. While, in the intestine, an increase in the fish fed at 58 \u00b5g\/g of fish was observed. In muscle, a slight decrease at 29 \u00b5g\/g was obtained. Additionally, copper concentrations in the pond water after 15 days of feeding with the [Cu(NN1)2](ClO4) complex showed the highest levels of copper. Finally, the effect of the administration of [Cu(NN1)2](ClO4) for 15 days at 58 \u00b5g\/g of fish was evaluated against F. psychrophilum, where a 75% survival was obtained during 20 days of challenge.<\/jats:p>","DOI":"10.3390\/microorganisms10112296","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T03:09:30Z","timestamp":1669000170000},"page":"2296","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protective Effect of [Cu(NN1)2](ClO4) Complex in Rainbow Trout Challenged against Flavobacterium psychrophilum"],"prefix":"10.3390","volume":"10","author":[{"given":"Maialen","family":"Aldabaldetrecu","sequence":"first","affiliation":[{"name":"Laboratory of Coordination Compounds and Supramolecularity, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mick","family":"Parra","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products Chemistry, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"},{"name":"Laboratory of Bacterial Metagenomic, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9170002, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarita","family":"Soto-Aguilera","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products Chemistry, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4689-3874","authenticated-orcid":false,"given":"Pablo","family":"Arce","sequence":"additional","affiliation":[{"name":"Laboratory of Coordination Compounds and Supramolecularity, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amaya Paz de la Vega","family":"Quiroz","sequence":"additional","affiliation":[{"name":"Laboratory of Electroanalysis, Department of Materials Chemistry, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5747-2357","authenticated-orcid":false,"given":"Rodrigo","family":"Segura","sequence":"additional","affiliation":[{"name":"Laboratory of Electroanalysis, Department of Materials Chemistry, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4573-6460","authenticated-orcid":false,"given":"Mario","family":"Tello","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Metagenomic, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9170002, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9827-5162","authenticated-orcid":false,"given":"Juan","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Laboratory of Coordination Compounds and Supramolecularity, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5292-0480","authenticated-orcid":false,"given":"Brenda","family":"Modak","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products Chemistry, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,19]]},"reference":[{"key":"ref_1","unstructured":"FAO (2020). The State of World Fisheries and Aquaculture 2020: Sustainability in Action, FAO. The State of World Fisheries and Aquaculture (SOFIA)."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.3389\/fpls.2021.718775","article-title":"Plant-Produced Vaccines: Future Applications in Aquaculture","volume":"12","author":"Su","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1111\/j.1439-0396.2012.01301.x","article-title":"Aquaculture and Stress Management: A Review of Probiotic Intervention","volume":"97","author":"Mohapatra","year":"2013","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1365-2761.2004.00598.x","article-title":"Flavobacterium Psychrophilum in Rainbow Trout, Oncorhynchus Mykiss (Walbaum), Hatcheries: Studies on Broodstock, Eggs, Fry and Environment","volume":"28","author":"Madsen","year":"2005","journal-title":"J. Fish Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jare.2014.10.009","article-title":"Emerging Flavobacterial Infections in Fish: A Review","volume":"6","author":"Loch","year":"2015","journal-title":"J. Adv. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"40","DOI":"10.2174\/1874401X01104010040","article-title":"A Review of Flavobacterium Psychrophilum Biology, Clinical Signs, and Bacterial Cold Water Disease Prevention and Treat","volume":"4","author":"Barnes","year":"2011","journal-title":"Open Fish Sci. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.jare.2010.04.001","article-title":"Bacterial Coldwater Disease of Fishes Caused by Flavobacterium Psychrophilum","volume":"2","author":"Starliper","year":"2011","journal-title":"J. Adv. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/jfd.12944","article-title":"Erythromycin and Florfenicol Treatment of Rainbow Trout Oncorhynchus Mykiss (Walbaum) Experimentally Infected with Flavobacterium Psychrophilum","volume":"42","author":"Jarau","year":"2019","journal-title":"J. Fish Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Savic, S. (2018). Antibiotics in Chilean Aquaculture: A Review. Antibiotic Use in Animals, InTech.","DOI":"10.5772\/intechopen.68438"},{"key":"ref_10","first-page":"38","article-title":"Antimicrobial Susceptibility and Plasmid Profiles of Flavobacterium Psychrophilum Strains Isolated in Chile","volume":"354","author":"Evrard","year":"2012","journal-title":"Aquaculture"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.3389\/fmicb.2016.01880","article-title":"Antimicrobial Susceptibility of Flavobacterium Psychrophilum from Chilean Salmon Farms and Their Epidemiological Cut-Off Values Using Agar Dilution and Disk Diffusion Methods","volume":"7","author":"Miranda","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1089\/mdr.2016.0103","article-title":"Antimicrobial Susceptibilities of Flavobacterium Psychrophilum Isolates from the Great Lakes Basin, Michigan","volume":"23","author":"Loch","year":"2017","journal-title":"Microb. Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/jfd.12730","article-title":"Antimicrobial Susceptibility of Flavobacterium Psychrophilum Isolates from the United Kingdom","volume":"41","author":"Ngo","year":"2018","journal-title":"J. Fish Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"734293","DOI":"10.1016\/j.aquaculture.2019.734293","article-title":"Antimicrobial Resistance and Resistance Genes in Flavobacterium Psychrophilum Isolates from Turkey","volume":"512","author":"Saticioglu","year":"2019","journal-title":"Aquaculture"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/jfd.12336","article-title":"Epidemiological Cut-off Values for Flavobacterium Psychrophilum MIC Data Generated by a Standard Test Protocol","volume":"39","author":"Smith","year":"2016","journal-title":"J. Fish Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1080\/13543776.2020.1853101","article-title":"Natural Compounds and Extracts as Novel Antimicrobial Agents","volume":"30","author":"Guglielmi","year":"2020","journal-title":"Expert Opin. Ther. Pat."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9162","DOI":"10.1021\/acs.chemrev.6b00184","article-title":"Plant Natural Products Targeting Bacterial Virulence Factors","volume":"116","author":"Silva","year":"2016","journal-title":"Chem. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s11101-006-9040-2","article-title":"Biosynthesis of Coumarins in Plants: A Major Pathway Still to Be Unravelled for Cytochrome P450 Enzymes","volume":"5","author":"Bourgaud","year":"2006","journal-title":"Phytochem. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bilal, M., Munir, H., and Iqbal, H.M.N. (2020). Potentialities of Medicinal Plant Extracts Against Biofilm-Forming Bacteria. Microbial Biofilms, CRC Press.","DOI":"10.1201\/9780367415075-12"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Yeu, J.-E., Lee, H.-G., Park, G.-Y., Lee, J., and Kang, M.-S. (2021). Antimicrobial and Antibiofilm Activities of Weissella Cibaria against Pathogens of Upper Respiratory Tract Infections. Microorganisms, 9.","DOI":"10.3390\/microorganisms9061181"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Pancu, D.F., Scurtu, A., Macasoi, I.G., Marti, D., Mioc, M., Soica, C., Coricovac, D., Horhat, D., Poenaru, M., and Dehelean, C. (2021). Antibiotics: Conventional Therapy and Natural Compounds with Antibacterial Activity\u2014A Pharmaco-Toxicological Screening. Antibiotics, 10.","DOI":"10.3390\/antibiotics10040401"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1016\/j.ejmech.2010.01.033","article-title":"New Cu(II), Co(II), Ni(II) Complexes with Aroyl-Hydrazone Based Ligand. Synthesis, Spectroscopic Characterization and in Vitro Antibacterial Evaluation","volume":"45","author":"Angelusiu","year":"2010","journal-title":"Eur. J. Med. Chem."},{"key":"ref_23","first-page":"883","article-title":"Synthesis, Spectroscopic, Thermal Characterization, and Antimicrobial Activity of Miconazole Drug and Its Metal Complexes","volume":"109","author":"Mohamed","year":"2011","journal-title":"J. Therm. Anal. Calorim."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Aldabaldetrecu, M., Parra, M., Soto, S., Arce, P., Tello, M., Guerrero, J., and Modak, B. (2020). New Copper(I) Complex with a Coumarin as Ligand with Antibacterial Activity against Flavobacterium Psychrophilum. Molecules, 25.","DOI":"10.3390\/molecules25143183"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Strepparava, N., Wahli, T., Segner, H., and Petrini, O. (2014). Detection and Quantification of Flavobacterium Psychrophilum in Water and Fish Tissue Samples by Quantitative Real Time PCR. BMC Microbiol., 14.","DOI":"10.1186\/1471-2180-14-105"},{"key":"ref_26","unstructured":"United States Environmental Protection Agency (2007). Aquatic Life Ambient Freshwater Quality Criteria\u2014Copper-2007 Revision: CAS Registry Number 7440-50-8."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.cbpc.2006.04.012","article-title":"Gastrointestinal Assimilation of Cu during Digestion of a Single Meal in the Freshwater Rainbow Trout (Oncorhynchus Mykiss)","volume":"143","author":"Nadella","year":"2006","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S1532-0456(02)00078-9","article-title":"Bioavailability and Toxicity of Dietborne Copper and Zinc to Fish","volume":"132","author":"Clearwater","year":"2002","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1080\/17460441.2019.1593367","article-title":"Discovery of Natural Products with Metal-Binding Properties as Promising Antibacterial Agents","volume":"14","author":"Dandawate","year":"2019","journal-title":"Expert Opin. Drug Discov."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"001363","DOI":"10.1099\/jmm.0.001363","article-title":"Evaluation of Metal-Based Antimicrobial Compounds for the Treatment of Bacterial Pathogens","volume":"70","author":"Evans","year":"2021","journal-title":"J. Med. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.aquaculture.2015.12.029","article-title":"Effect of Dietary Inorganic Copper on Growth Performance and Hematological Profile of Nile Tilapia Subjected to Heat-Induced Stress","volume":"454","author":"Damasceno","year":"2016","journal-title":"Aquaculture"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.aquaculture.2017.09.037","article-title":"Comparison of Copper Bioavailability in Copper-Methionine, Nano-Copper Oxide and Copper Sulfate Additives in the Diet of Russian Sturgeon Acipenser Gueldenstaedtii","volume":"482","author":"Wang","year":"2018","journal-title":"Aquaculture"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1111\/anu.12376","article-title":"Effects of Dietary Copper Nanoparticles and Vitamin C Supplementations on Growth Performance, Immune Response and Stress Resistance of Red Sea Bream, Pagrus Major","volume":"23","author":"Dawood","year":"2017","journal-title":"Aquacult. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.aquaculture.2016.01.007","article-title":"Effect of Different Levels of Dietary Copper Nanoparticles and Copper Sulfate on Growth Performance, Blood Biochemical Profiles, Antioxidant Status and Immune Response of Red Sea Bream (Pagrus Major)","volume":"455","author":"Dawood","year":"2016","journal-title":"Aquaculture"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"(1998). Lorentzen; Maage; Julshamn Supplementing Copper to a Fish Meal Based Diet Fed to Atlantic Salmon Parr Affects Liver Copper and Selenium Concentrations. Aquac. Nutr., 4, 67\u201372.","DOI":"10.1046\/j.1365-2095.1998.00046.x"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0166-445X(02)00101-7","article-title":"The Influence of Ration Size on Copper Homeostasis during Sublethal Dietary Copper Exposure in Juvenile Rainbow Trout, Oncorhynchus Mykiss","volume":"62","author":"Kamunde","year":"2003","journal-title":"Aquat. Toxicol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"736350","DOI":"10.1016\/j.aquaculture.2021.736350","article-title":"Toxic, Physiological, Histomorphological, Growth Performance and Antiparasitic Effects of Copper Sulphate in Fish Aquaculture","volume":"535","year":"2021","journal-title":"Aquaculture"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1111\/are.13012","article-title":"Dietary Copper Effects Survival of Channel Catfish Challenged with Flavobacterium Columnare","volume":"48","author":"Farmer","year":"2017","journal-title":"Aquac. Res."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2296\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:21:47Z","timestamp":1760145707000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/11\/2296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,19]]},"references-count":38,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["microorganisms10112296"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10112296","relation":{},"ISSN":["2076-2607"],"issn-type":[{"type":"electronic","value":"2076-2607"}],"subject":[],"published":{"date-parts":[[2022,11,19]]}}}