{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T03:03:24Z","timestamp":1792206204007,"version":"4.4.0"},"reference-count":69,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T00:00:00Z","timestamp":1669593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Li Ka Shing Foundation","award":["2022M712793"],"award-info":[{"award-number":["2022M712793"]}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022M712793"],"award-info":[{"award-number":["2022M712793"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Microecological preparation could relieve Enterotoxigenic Escherichia coli (ETEC) K88-induced diarrhea in piglets, but which bacteria play a key role and the mitigation mechanism have not been fully clarified. In this study, 36 male mice were randomly divided into six groups (CON, K88, BK (Bifidobacterium longum + K88), LK (Lactobacillus plantarum + K88), PK (Pediococcus acidilactici + K88), and MK (mixed strains + K88)) to explore the prevention mechanisms. Three probiotic strains and their mixtures (TPSM) significantly relieved the weight loss and restored the ratio of villus height to crypt depth in the jejunum. Except for Bifidobacterium longum, other strains significantly decreased interleukin (IL)-1\u03b2, IL-6 and tumor necrosis factor-\u03b1 (TNF-\u03b1) in mice serum. The TPSM treatment significantly downregulated the mRNA expression of the inflammatory cytokines and the Toll-like receptor and downstream gene (TLR4, MyD88, NF-\u03baB) in jejunum induced by ETEC. Furthermore, the TPSM could restore dysbiosis of the intestinal microbiota caused by ETEC. The intestinal microbiota analysis demonstrated that Bifidobacterium longum enriched the Bifidobacterium genus (p &lt; 0.05), Lactobacillus plantarum enriched the Lactobacillus genus (p &lt; 0.05), Pediococcus acidilactici enriched the Coriobacteriaceae_UCG-002 and Christensenellaceae_R-7_group genus (p &lt; 0.05), mixed bacteria enriched the Akkermansia genus (p &lt; 0.05), but ETEC enriched the Desulfovibrio genus (p &lt; 0.05). Meanwhile, the starch and sucrose metabolism, galactose and fructose metabolism, mannose metabolism and ABC transporters were increased with probiotics pre-treatment (p &lt; 0.05). To sum up, the microecological preparation alleviated ETEC-induced diarrhea by regulating the immune response, rebalancing intestinal microbiota and improving carbohydrate metabolism.<\/jats:p>","DOI":"10.3390\/microorganisms10122350","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T06:04:36Z","timestamp":1669615476000},"page":"2350","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Bifidobacterium longum, Lactobacillus plantarum and Pediococcus acidilactici Reversed ETEC-Inducing Intestinal Inflammation in Mice"],"prefix":"10.3390","volume":"10","author":[{"given":"Wentao","family":"Li","sequence":"first","affiliation":[{"name":"National Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Institute of Feed Science, College of Animal Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Kai","sequence":"additional","affiliation":[{"name":"National Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Institute of Feed Science, College of Animal Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zipeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Institute of Feed Science, College of Animal Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"He","sequence":"additional","affiliation":[{"name":"National Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Institute of Feed Science, College of Animal 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhen","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, China"},{"name":"Institute of Feed Science, College of Animal Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, 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Travel Med."},{"key":"ref_3","unstructured":"GBD 2016 Diarrhoeal Disease Collaborators (2018). Estimates of the global, regional, and national morbidity, mortality, and aetiologies of diarrhoea in 195 countries: A systematic analysis for the Global Burden of Disease Study 2016. Lancet Infect Dis., 18, 1211\u20131228."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.tim.2017.05.004","article-title":"Intestinal Microbiota Dysbiosis in Postweaning Piglets: Understanding the Keys to Health","volume":"25","author":"Gresse","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00526-e18","DOI":"10.1128\/IAI.00526-18","article-title":"Molecular Determinants of Enterotoxigenic Escherichia coli Heat-Stable Toxin Secretion and Delivery","volume":"86","author":"Zhu","year":"2018","journal-title":"Infect. Immun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"131","DOI":"10.54846\/jshap\/542","article-title":"Effect of weaning age on nursery pig and sow reproductive performance","volume":"16","author":"Alison","year":"2008","journal-title":"J. Swine Health Prod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/s40104-019-0366-1","article-title":"Effect of mucin 4 allele on susceptibility to experimental infection with enterotoxigenic F4 Escherichia coli in pigs fed experimental diets","volume":"10","author":"Sterndale","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_8","first-page":"679","article-title":"Fimbriae of animal-originated enterotoxigenic Escherichia coli\u2014A review","volume":"52","author":"Zhou","year":"2012","journal-title":"Acta Microbiol. Sin."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1002\/jobm.201400901","article-title":"F4+ enterotoxigenic Escherichia coli (ETEC) adhesion mediated by the major fimbrial subunit FaeG","volume":"55","author":"Xia","year":"2015","journal-title":"J. Basic Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0378-1135(99)00174-1","article-title":"The F4 fimbrial antigen of Escherichia coli and its receptors","volume":"71","author":"Broeck","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_11","first-page":"CD006095","article-title":"Probiotics for the prevention of Clostridium difficile-associated diarrhea in adults and children","volume":"12","author":"Goldenberg","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1002\/jgh3.12390","article-title":"Lactulose may reduce Clostridium difficile-related diarrhea among patients receiving antibiotics","volume":"4","author":"Maltz","year":"2020","journal-title":"JGH Open"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13567-018-0511-4","article-title":"Lysozyme improves intestinal performance and protects against enterotoxigenic Escherichia coli infection in neonatal piglets","volume":"49","author":"Huang","year":"2018","journal-title":"Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"93","DOI":"10.5713\/ajas.2013.13411","article-title":"Comparison of single and blend acidifiers as alternative to antibiotics on growth performance, fecal microflora, and humoral immunity in weaned piglets","volume":"27","author":"Ahmed","year":"2014","journal-title":"Asian Austral. J. Anim."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Abiala, M., Olayiwola, J., Babatunde, O., Aiyelaagbe, O., and Akinyemi, S. (2016). Evaluation of therapeutic potentials of plant extracts against poultry bacteria threatening public health. BMC Complem. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1399-z"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1002\/med.21542","article-title":"Antimicrobial peptides: Promising alternatives in the post feeding antibiotic era","volume":"39","author":"Wang","year":"2019","journal-title":"Med. Res. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nature09646","article-title":"Bifidobacteria can protect from enteropathogenic infection through production of acetate","volume":"469","author":"Fukuda","year":"2011","journal-title":"Nature"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e0312120","DOI":"10.1128\/AEM.03121-20","article-title":"Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions","volume":"87","author":"Hrala","year":"2021","journal-title":"Appl. Environ. Microb."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1159\/000342079","article-title":"Probiotic mechanisms of action","volume":"61","author":"Gil","year":"2012","journal-title":"Ann. Nutr. Metab."},{"key":"ref_20","first-page":"1831339","article-title":"Molecular insights into probiotic mechanisms of action employed against intestinal pathogenic bacteria","volume":"12","author":"Deane","year":"2020","journal-title":"Intest. Microbes"},{"key":"ref_21","first-page":"155300","article-title":"Application Progress of Autochthonous Microbiota in Broiler Feed","volume":"5","author":"Ni","year":"2021","journal-title":"Feed Indus."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3683","DOI":"10.1111\/1462-2920.14705","article-title":"Bifidobacterium bifidum and the infant intestinal microbiota: An intriguing case of microbe-host co-evolution","volume":"21","author":"Duranti","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_23","first-page":"925","article-title":"Bifidobacteria and their role as members of the human intestinal microbiota","volume":"7","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"724401","DOI":"10.3389\/fcimb.2021.724401","article-title":"Lactobacillus rhamnosus LB1 Alleviates Enterotoxigenic Escherichia coli-Induced Adverse Effects in Piglets by Improving Host Immune Response and Anti-Oxidation Stress and Restoring Intestinal Integrity","volume":"11","author":"Wu","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"746585","DOI":"10.3389\/fimmu.2021.746585","article-title":"Lactobacillus plantarum CCFM1143 Alleviates Chronic Diarrhea via Inflammation Regulation and Intestinal Microbiota Modulation: A Double-Blind, Randomized, Placebo-Controlled Study","volume":"12","author":"Yang","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7340312","DOI":"10.1155\/2020\/7340312","article-title":"Pediococcus acidilactici P25 Protected Caenorhabditis elegans against Enterotoxigenic Escherichia coli K88 Infection and Transcriptomic Analysis of Its Potential Mechanisms","volume":"2020","author":"Tan","year":"2020","journal-title":"Biomed. Res. Int."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mao, X., Gu, C., Hu, H., Tang, J., Chen, D., Yu, B., He, J., Yu, J., Luo, J., and Tian, G. (2016). Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146312"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7884","DOI":"10.1021\/acs.jafc.1c02257","article-title":"Oleanolic Acid Targets the Intestinal-Liver Axis to Alleviate Metabolic Disorders and Hepatic Steatosis","volume":"69","author":"Xue","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1002\/jsfa.11410","article-title":"Oleanolic acid reshapes the intestinal microbiota and alters immune-related gene expression of intestinal epithelial cells","volume":"102","author":"Xue","year":"2022","journal-title":"J. Sci. Food Agric."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Aluri, J., Cooper, M.A., and Schuettpelz, L.G. (2021). Toll-Like Receptor Signaling in the Establishment and Function of the Immune System. Cells, 10.","DOI":"10.3390\/cells10061374"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3389\/fimmu.2017.00085","article-title":"NF-\u03baB Links TLR2 and PAR1 to Soluble Immunomodulator Factor Secretion in Human Platelets","volume":"8","author":"Damien","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3920\/BM2018.0066","article-title":"Mechanisms and immunomodulatory properties of pre- and probiotics","volume":"10","author":"Peters","year":"2019","journal-title":"Benef. Microbes"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.3389\/fmicb.2017.01885","article-title":"Lactobacillus plantarum BSGP201683 Isolated from Giant Panda Feces Attenuated Inflammation and Improved Intestinal Microflora in Mice Challenged with Enterotoxigenic Escherichia coli","volume":"8","author":"Liu","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_34","first-page":"169","article-title":"Virulence factors of enterotoxigenic E. coli studied in the infant mouse model","volume":"14","author":"Bertin","year":"1983","journal-title":"Ann. Rech. Vet."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1007\/s00424-016-1836-3","article-title":"Defective small intestinal anion secretion, dipeptide absorption, and intestinal failure in suckling NBCe1-deficient mice","volume":"468","author":"Yu","year":"2016","journal-title":"Pflugers Arch."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1007\/s12602-020-09725-9","article-title":"Human \u03b2-Defensin 118 Attenuates Escherichia coli K88-Induced Inflammation and Intestinal Injury in Mice","volume":"13","author":"Lin","year":"2021","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6867962","DOI":"10.1155\/2021\/6867962","article-title":"Lactobacillus plantarum and Lactobacillus brevis Alleviate Intestinal Inflammation and Microbial Disorder Induced by ETEC in a Murine Model","volume":"2021","author":"Han","year":"2021","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/j.micinf.2014.09.005","article-title":"Mouse intestinal innate immune responses altered by Enterotoxigenic Escherichia coli (ETEC) infection","volume":"16","author":"Ren","year":"2014","journal-title":"Microbes Infect."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.2217\/fmb.15.17","article-title":"Role of virulence factors on host inflammatory response induced by diarrheagenic Escherichia coli pathotypes","volume":"10","year":"2015","journal-title":"Future Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1039\/C8RA09247H","article-title":"The anti-diarrhea activity of red algae-originated sulphated polysaccharides on ETEC-K88 infected mice","volume":"9","author":"Liu","year":"2019","journal-title":"RSC Adv."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"7210","DOI":"10.1038\/s41598-022-11340-3","article-title":"Microencapsulated probiotic Lactiplantibacillus plantarum and\/or Pediococcus acidilactici strains ameliorate diarrhoea in piglets challenged with Enterotoxigenic Escherichia coli","volume":"12","author":"Pupa","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ijbiomac.2020.12.073","article-title":"Role of immunoglobulin and antibodies in disease management","volume":"169","author":"Megha","year":"2021","journal-title":"Int. J. Bio. Macromol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"17485","DOI":"10.1038\/s41598-018-35702-y","article-title":"Lactobacillus reuteri HCM2 protects mice against Enterotoxigenic Escherichia coli through modulation of intestinal microbiota","volume":"8","author":"Wang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1079\/AHR2005105","article-title":"Escherichia coli in postweaning diarrhea in pigs: An update on bacterial types, pathogenesis, and prevention strategies","volume":"6","author":"Fairbrother","year":"2005","journal-title":"Anim. Health Res. Rev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"104163","DOI":"10.1016\/j.micpath.2020.104163","article-title":"In Vitro probiotic properties of Pediococcus pentosaceus L1 and its effects on enterotoxigenic Escherichia coli-induced inflammatory responses in porcine intestinal epithelial cells","volume":"144","author":"Yin","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1615\/CritRevImmunol.v30.i1.40","article-title":"Induction of cytokines and chemokines by Toll-like receptor signaling: Strategies for control of inflammation","volume":"30","author":"Zeytun","year":"2010","journal-title":"Crit. Rev. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2940","DOI":"10.1128\/IAI.73.5.2940-2950.2005","article-title":"Differential induction of the toll-like receptor 4-MyD88-dependent and -independent signaling pathways by endotoxins","volume":"73","author":"Zughaier","year":"2005","journal-title":"Infect Immun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"683863","DOI":"10.3389\/fvets.2021.683863","article-title":"Clostridium butyricum helps to alleviate inflammation in weaned piglets challenged with Enterotoxigenic Escherichia coli K88","volume":"8","author":"Li","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"825","DOI":"10.3892\/mmr.2016.5302","article-title":"Enterotoxigenic Escherichia coli infection alters intestinal immunity in mice","volume":"14","author":"Yang","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"27","DOI":"10.2152\/jmi.63.27","article-title":"The intestinal microbiota and its role in human health and disease","volume":"63","author":"Kataoka","year":"2016","journal-title":"J. Med. Investig."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10362","DOI":"10.1039\/D0FO02670K","article-title":"Lactobacillus plantarum relieves diarrhea caused by enterotoxin-producing Escherichia coli through inflammation modulation and intestinal microbiota regulation","volume":"11","author":"Yue","year":"2020","journal-title":"Food Funct."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104856","DOI":"10.1016\/j.micpath.2021.104856","article-title":"L. plantarum, and L. rhamnosus alleviated Enterohaemorrhagic Escherichia coli-induced diarrhoea in mice by regulating intestinal microbiota","volume":"154","author":"Hu","year":"2021","journal-title":"Microb. Pathog."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"79329","DOI":"10.1039\/C5RA16338B","article-title":"An integrated metabonomics and microbiology analysis of host-microbiota metabolic interactions in rats with coptis chinensis-induced diarrhea","volume":"5","author":"Li","year":"2015","journal-title":"RSC Adv."},{"key":"ref_54","first-page":"824","article-title":"Escherichia coli O101-induced diarrhea develops intestinal microbial dysbiosis in rats","volume":"17","author":"Sun","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"9351507","DOI":"10.1155\/2017\/9351507","article-title":"Proteobacteria: A Common Factor in Human Diseases","volume":"2017","author":"Rizzatti","year":"2017","journal-title":"Biomed. Res. Int."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tibtech.2015.06.011","article-title":"Proteobacteria: Microbial signature of dysbiosis in intestinal microbiota","volume":"33","author":"Shin","year":"2015","journal-title":"Trends Biotechnol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1515\/biol-2018-0057","article-title":"Analysis of Physiological Parameters of Desulfovibrio Strains from Individuals with Colitis","volume":"13","author":"Kushkevych","year":"2019","journal-title":"Open Life Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.micpath.2017.09.054","article-title":"Sulphate-reducing bacteria from ulcerative colitis patients induce apoptosis of gastrointestinal epithelial cells","volume":"112","author":"Coutinho","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Guo, W., Mao, B., Cui, S., Tang, X., Zhang, Q., Zhao, J., and Zhang, H. (2022). Protective effects of a novel probiotic Bifidobacterium pseudolongum on the intestinal barrier of colitis mice via modulating the Ppar\u03b3\/STAT3 pathway and intestinal microbiota. Foods, 11.","DOI":"10.3390\/foods11111551"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1093\/nutrit\/nuw067","article-title":"Role of short-chain fatty acids in colonic inflammation, carcinogenesis, and mucosal protection and healing","volume":"75","author":"Dejong","year":"2017","journal-title":"Nutr. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Waters, J.L., and Ley, R.E. (2019). The human intestinal bacteria Christensenellaceae are widespread, heritable, and associated with health. BMC Biol., 17.","DOI":"10.1186\/s12915-019-0699-4"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1093\/femsec\/fix153","article-title":"Identification of universal intestinal microbial biomarkers of common human intestinal diseases by meta-analysis","volume":"93","author":"Mancabelli","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1038\/s41591-019-0495-2","article-title":"Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: A proof-of-concept exploratory study","volume":"25","author":"Depommier","year":"2019","journal-title":"Nat. Med."},{"key":"ref_64","first-page":"8075","article-title":"Akkermansia muciniphila: Key player in metabolic and gastrointestinal disorders","volume":"23","author":"Macchione","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_65","first-page":"1988","article-title":"A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8(+) T cells in mice","volume":"69","author":"Wang","year":"2020","journal-title":"Intestinal"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e00501","DOI":"10.1128\/mSphere.00501-17","article-title":"Persistence of Supplemented Bifidobacterium longum subsp. infantis EVC001 in Breastfed Infants","volume":"2","author":"Frese","year":"2017","journal-title":"mSphere"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Ehrlich, A.M., Pacheco, A.R., Henrick, B.M., Taft, D., Xu, G., Huda, M.N., Mishchuk, D., Goodson, M.L., Slupsky, C., and Barile, D. (2020). Indole-3-lactic acid associated with Bifidobacterium-dominated microbiota significantly decreases inflammation in intestinal epithelial cells. BMC Microbiol., 20.","DOI":"10.1186\/s12866-020-02023-y"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1007\/s00424-020-02379-0","article-title":"Transporters of glucose and other carbohydrates in bacteria","volume":"472","author":"Jeckelmann","year":"2020","journal-title":"Pflugers Arch."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.copbio.2012.11.007","article-title":"Microbe-microbe interactions in mixed culture food fermentations","volume":"24","author":"Smid","year":"2013","journal-title":"Curr. Opin. Biotechnol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/12\/2350\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:28:22Z","timestamp":1760146102000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/10\/12\/2350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,28]]},"references-count":69,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["microorganisms10122350"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms10122350","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,28]]}}}