{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T18:54:31Z","timestamp":1773687271272,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordination for the Improvement of Higher Education Personnel","award":["Pr\u00eamio CAPES 2015\u20140774\/2017"],"award-info":[{"award-number":["Pr\u00eamio CAPES 2015\u20140774\/2017"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel","award":["406402\/2018-3"],"award-info":[{"award-number":["406402\/2018-3"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel","award":["APQ-00524-17"],"award-info":[{"award-number":["APQ-00524-17"]}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["Pr\u00eamio CAPES 2015\u20140774\/2017"],"award-info":[{"award-number":["Pr\u00eamio CAPES 2015\u20140774\/2017"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["406402\/2018-3"],"award-info":[{"award-number":["406402\/2018-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["APQ-00524-17"],"award-info":[{"award-number":["APQ-00524-17"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["Pr\u00eamio CAPES 2015\u20140774\/2017"],"award-info":[{"award-number":["Pr\u00eamio CAPES 2015\u20140774\/2017"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["406402\/2018-3"],"award-info":[{"award-number":["406402\/2018-3"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-00524-17"],"award-info":[{"award-number":["APQ-00524-17"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pr\u00f3-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq\/UFMG)","award":["Pr\u00eamio CAPES 2015\u20140774\/2017"],"award-info":[{"award-number":["Pr\u00eamio CAPES 2015\u20140774\/2017"]}]},{"name":"Pr\u00f3-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq\/UFMG)","award":["406402\/2018-3"],"award-info":[{"award-number":["406402\/2018-3"]}]},{"name":"Pr\u00f3-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq\/UFMG)","award":["APQ-00524-17"],"award-info":[{"award-number":["APQ-00524-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Despite its clinical relevance, the pathogenesis of canine pyometra remains poorly understood. To date, it is recognized as a non-transmissible infectious disease. In this study, the simultaneous occurrence of pyometra and Escherichia coli in two cohabitant female dogs underwent in-depth investigation due to the hypothesis of transmission between these animals. Two 5-year-old Chow Chow dogs (namely, dogs 23 and 24\u2014D23 and D24) were referred to a veterinary hospital with suspected pyometra. Both animals showed prostration, anorexia, and purulent vulvar discharge over a 1-week period. After ovariohysterectomy, uterine tissue, uterine contents, and rectal swabs were collected for histopathological and microbiological analysis. Uterine histology demonstrated purulent material and multifocal necrosis with endometrial ulceration, and a morphological diagnosis of pyometra was confirmed. Furthermore, E. coli from the same phylogroup (B2) and positive for the same virulence factors with the same antimicrobial susceptibility profile was isolated from the uterine contents of both dogs and the rectum of D23. Conversely, the E. coli strains recovered from D24 differed in phylogroup (one isolate), virulence factors (all three isolates), and antimicrobial susceptibility (all three isolates). Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) suggested that all isolates from the uterine content of both dogs and the rectal swab of D23 were 100% the same, but different from all isolates in the rectal swab of D24. One isolate from the uterine content of each animal as well as rectal swabs were subjected to whole-genome sequencing (WGS). Both whole-genome multilocus sequence typing(wgMLST) and single-nucleotide polymorphism (SNP) analysis supported the hypothesis that the isolates from the uterine content of both animals and the rectal swab of D23 were clonal. Taken together, these clinical features, pathology, microbiology, and molecular findings suggest, to the best of our knowledge, the first transmission of E. coli associated with pyometra between two animals. These results could impact the management of sites where several females cohabit in the same local area such as kennels.<\/jats:p>","DOI":"10.3390\/microorganisms10122465","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T02:22:41Z","timestamp":1670984561000},"page":"2465","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transmission of Escherichia coli Causing Pyometra between Two Female Dogs"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4770-5134","authenticated-orcid":false,"given":"Rafael Gariglio Clark","family":"Xavier","sequence":"first","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarissa Helena","family":"Santana","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4301-5822","authenticated-orcid":false,"given":"Paloma Helena Sanches","family":"da Silva","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-1882","authenticated-orcid":false,"given":"Fl\u00e1via Figueira","family":"Aburjaile","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe Luiz","family":"Pereira","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-6842","authenticated-orcid":false,"given":"Henrique C\u00e9sar Pereira","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Maria Coletto","family":"Freitas","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4830-0470","authenticated-orcid":false,"given":"Renato Lima","family":"Santos","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0074-2488","authenticated-orcid":false,"given":"Rodrigo Ot\u00e1vio Silveira","family":"Silva","sequence":"additional","affiliation":[{"name":"Veterinary School, Federal University of Minas Gerais, Ant\u00f4nio Carlos Avenue 6627, Belo Horizonte 31270-090, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.cvsm.2018.03.001","article-title":"Pyometra in Small Animals","volume":"48","author":"Hagman","year":"2018","journal-title":"Vet. Clin. Small Anim. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.cvsm.2022.01.004","article-title":"Pyometra in Small Animals 2.0","volume":"52","author":"Hagman","year":"2022","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/rda.12302","article-title":"Medical Treatment for Pyometra in Dogs","volume":"49","author":"Fieni","year":"2014","journal-title":"Reprod. Domest. Anim."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Jitpean, S., Str\u00f6m-Holst, B., Emanuelson, U., H\u00f6glund, O.V., Pettersson, A., Alneryd-Bull, C., and Hagman, R. (2014). Outcome of Pyometra in Female Dogs and Predictors of Peritonitis and Prolonged Postoperative Hospitalization in Surgically Treated Cases. BMC Vet. Res., 10.","DOI":"10.1186\/1746-6148-10-6"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1080\/01652176.2015.1068963","article-title":"Phylo-Typing of Clinical Escherichia coli Isolates Originating from Bovine Mastitis and Canine Pyometra and Urinary Tract Infection by Means of Quadruplex PCR","volume":"35","author":"Kaya","year":"2015","journal-title":"Vet. Q."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0378-1135(03)00063-4","article-title":"Uropathogenic Virulence Factors in Isolates of Escherichia coli from Clinical Cases of Canine Pyometra and Feces of Healthy Bitches","volume":"94","author":"Chen","year":"2003","journal-title":"Vet. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.theriogenology.2008.04.041","article-title":"Cystic Endometrial Hyperplasia, Pseudo-Placentational Endometrial Hyperplasia, and Other Cystic Conditions of the Canine and Feline Uterus","volume":"70","author":"Schlafer","year":"2008","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.24070\/bjvp.1983-0246.v14i1p1-8","article-title":"Canine Pyometra\u2014An Update and Revision of Diagnostic Terminology","volume":"14","author":"Santana","year":"2021","journal-title":"Braz. J. Vet. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jcpa.2020.09.002","article-title":"Association of Pseudoplacentational Endometrial Hyperplasia and Pyometra in Dogs","volume":"180","author":"Santana","year":"2020","journal-title":"J. Comp. Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1128\/aem.62.9.3350-3354.1996","article-title":"Confirmational Identification of Escherichia coli, a Comparison of Genotypic and Phenotypic Assays for Glutamate Decarboxylase and Beta-D-Glucuronidase","volume":"62","author":"McDaniels","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/1758-2229.12019","article-title":"The Clermont Escherichia coli Phylo-Typing Method Revisited: Improvement of Specificity and Detection of New Phylo-Groups","volume":"5","author":"Clermont","year":"2013","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1086\/315217","article-title":"Extended Virulence Genotypes of Escherichia coli Strains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise","volume":"181","author":"Johnson","year":"2000","journal-title":"J. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.rvsc.2008.07.018","article-title":"Virulence Factors in Escherichia coli Strains Isolated from Urinary Tract Infection and Pyometra Cases and from Feces of Healthy Dogs","volume":"86","author":"Siqueira","year":"2009","journal-title":"Res. Vet. Sci."},{"key":"ref_14","unstructured":"CLSI (2020). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, Clinical and Laboratory Standards Institute. [5th ed.]. CLSI supplement VET01S."},{"key":"ref_15","unstructured":"CLSI (2021). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [31st ed.]. CLSI supplement M100."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lopes, C.E., De Carli, S., Riboldi, C.I., De Lorenzo, C., Panziera, W., Driemeier, D., and Siqueira, F.M. (2021). Pet Pyometra: Correlating Bacteria Pathogenicity to Endometrial Histological Changes. Pathogens, 10.","DOI":"10.3390\/pathogens10070833"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Xavier, R.G.C., da Silva, P.H.S., Trindade, H.D., Carvalho, G.M., Nicolino, R.R., Freitas, P.M.C., and Silva, R.O.S. (2022). Characterization of Escherichia coli in Dogs with Pyometra and the Influence of Diet on the Intestinal Colonization of Extraintestinal Pathogenic E. coli (ExPEC). Vet. Sci., 9.","DOI":"10.3390\/vetsci9050245"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"104453","DOI":"10.1016\/j.meegid.2020.104453","article-title":"Insights on the Genetic Features of Endometrial Pathogenic Escherichia coli Strains from Pyometra in Companion Animals: Improving the Knowledge about Pathogenesis","volume":"85","author":"Lopes","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"979584","DOI":"10.1155\/2014\/979584","article-title":"Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains","volume":"2014","author":"Agostinho","year":"2014","journal-title":"Int. J. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.ijfoodmicro.2005.03.019","article-title":"Comparison of Four Molecular Typing Methods for the Differentiation of Salmonella spp.","volume":"105","author":"Lim","year":"2005","journal-title":"Int. J. Food Microbiol."},{"key":"ref_21","first-page":"73","article-title":"Molecular Typing of Salmonella spp. Isolated from Food Handlers and Animals in Nigeria","volume":"2","author":"Smith","year":"2011","journal-title":"Int. J. Mol. Epidemiol. Genet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.envres.2009.02.014","article-title":"Source Identification of Airborne Escherichia coli of Swine House Surroundings Using ERIC-PCR and REP-PCR","volume":"109","author":"Duan","year":"2009","journal-title":"Environ. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.vetmic.2014.01.021","article-title":"Genotypic and Phenotypic Comparison of Escherichia coli from Uterine Infections with Different Outcomes: Clinical Metritis in the Cow and Pyometra in the Bitch","volume":"170","author":"Henriques","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.bjm.2017.01.014","article-title":"Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR Analysis as a Reliable Evidence for Suspected Shigella spp. Outbreaks","volume":"49","author":"Bakhshi","year":"2018","journal-title":"Braz. J. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.jinf.2015.09.009","article-title":"Extra-Intestinal Pathogenic Escherichia coli (ExPEC): Disease, Carriage and Clones","volume":"71","author":"Dale","year":"2015","journal-title":"J. Infect."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1089\/cmb.2013.0084","article-title":"Assembling Single-Cell Genomes and Mini-Metagenomes from Chimeric MDA Products","volume":"20","author":"Nurk","year":"2013","journal-title":"J. Comput. Biol. J. Comput. Mol. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid Prokaryotic Genome Annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and Applications. BMC Bioinformatics, 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-Time Whole-Genome Sequencing for Routine Typing, Surveillance, and Outbreak Detection of Verotoxigenic Escherichia coli","volume":"52","author":"Joensen","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1093\/jac\/dkx217","article-title":"PointFinder: A Novel Web Tool for WGS-Based Detection of Antimicrobial Resistance Associated with Chromosomal Point Mutations in Bacterial Pathogens","volume":"72","author":"Zankari","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1093\/jac\/dkaa345","article-title":"ResFinder 4.0 for Predictions of Phenotypes from Genotypes","volume":"75","author":"Bortolaia","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e01269-20","DOI":"10.1128\/JCM.01269-20","article-title":"In Silico Genotyping of Escherichia coli Isolates for Extraintestinal Virulence Genes by Use of Whole-Genome Sequencing Data","volume":"58","author":"Johnson","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1128\/JCM.00008-15","article-title":"Rapid and Easy In Silico Serotyping of Escherichia coli Isolates by Use of Whole-Genome Sequencing Data","volume":"53","author":"Joensen","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/j.1365-2958.2006.05172.x","article-title":"Sex and Virulence in Escherichia coli: An Evolutionary Perspective","volume":"60","author":"Wirth","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Jaureguy, F., Landraud, L., Passet, V., Diancourt, L., Frapy, E., Guigon, G., Carbonnelle, E., Lortholary, O., Clermont, O., and Denamur, E. (2008). Phylogenetic and Genomic Diversity of Human Bacteremic Escherichia coli Strains. BMC Genomics, 9.","DOI":"10.1186\/1471-2164-9-560"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1128\/JCM.06094-11","article-title":"Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria","volume":"50","author":"Larsen","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Francisco, A.P., Bugalho, M., Ramirez, M., and Carri\u00e7o, J.A. (2009). Global Optimal EBURST Analysis of Multilocus Typing Data Using a Graphic Matroid Approach. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-152"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1093\/bioinformatics\/btw582","article-title":"PHYLOViZ 2.0: Providing Scalable Data Integration and Visualization for Multiple Phylogenetic Inference Methods","volume":"33","author":"Nascimento","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1038\/nbt.2522","article-title":"Updating Benchtop Sequencing Performance Comparison","volume":"31","author":"Sedlazeck","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.vetmic.2014.01.003","article-title":"Multidrug-Resistant Escherichia coli from Canine Urinary Tract Infections Tend to Have Commensal Phylotypes, Lower Prevalence of Virulence Determinants and AmpC-Replicons","volume":"169","author":"Wagner","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e00788-18","DOI":"10.1128\/JCM.00788-18","article-title":"Population Structure and Antimicrobial Resistance of Canine Uropathogenic Escherichia coli","volume":"56","author":"LeCuyer","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Flament-Simon, S.-C., de Toro, M., Garc\u00eda, V., Blanco, J.E., Blanco, M., Alonso, M.P., Goicoa, A., D\u00edaz-Gonz\u00e1lez, J., Nicolas-Chanoine, M.-H., and Blanco, J. (2020). Molecular Characteristics of Extraintestinal Pathogenic E. coli (ExPEC), Uropathogenic E. coli (UPEC), and Multidrug Resistant E. coli Isolated from Healthy Dogs in Spain. Whole Genome Sequencing of Canine ST372 Isolates and Comparison with Human Isolates Causing Extraintestinal Infections. Microorganisms, 8.","DOI":"10.20944\/preprints202009.0269.v1"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"108822","DOI":"10.1016\/j.vetmic.2020.108822","article-title":"Comprehensive Phenotypic and Genotypic Characterization and Comparison of Virulence, Biofilm, and Antimicrobial Resistance in Urinary Escherichia coli Isolated from Canines","volume":"249","author":"Gilbertie","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"108783","DOI":"10.1016\/j.vetmic.2020.108783","article-title":"Genomic Analysis of Phylogenetic Group B2 Extraintestinal Pathogenic E. coli Causing Infections in Dogs in Australia","volume":"248","author":"Kidsley","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"580","DOI":"10.3389\/fmicb.2020.00580","article-title":"Pathogenic Escherichia coli in Dogs Reveals the Predominance of ST372 and the Human-Associated ST73 Extra-Intestinal Lineages","volume":"11","author":"Valat","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/rda.12843","article-title":"Canine Pyometra: What Is New?","volume":"52","author":"Hagman","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1590\/S1517-83822008000300012","article-title":"Microbiological and Histopathological Aspects of Canine Pyometra","volume":"39","author":"Coggan","year":"2008","journal-title":"Braz. J. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1128\/IAI.69.3.1306-1314.2001","article-title":"Canine Feces as a Reservoir of Extraintestinal Pathogenic Escherichia coli","volume":"69","author":"Johnson","year":"2001","journal-title":"Infect. Immun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.cimid.2011.12.005","article-title":"Antimicrobial Resistance, Virulence Profiles, and Phylogenetic Groups of Fecal Escherichia coli Isolates: A Comparative Analysis between Dogs and Their Owners in Japan","volume":"35","author":"Harada","year":"2012","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/jam.13003","article-title":"DNA Fingerprinting Approaches to Trace Escherichia coli Sharing between Dogs and Owners","volume":"120","author":"Naziri","year":"2016","journal-title":"J. Appl. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Yasugi, M., Hatoya, S., Motooka, D., Matsumoto, Y., Shimamura, S., Tani, H., Furuya, M., Mie, K., Miyake, M., and Nakamura, S. (2021). Whole-genome analyses of extended-spectrum or AmpC \u03b2-lactamase-producing Escherichia coli isolates from companion dogs in Japan. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0246482"},{"key":"ref_52","first-page":"383","article-title":"A survey of the cystic hyperplasia-pyometra complex in the bitch","volume":"1","author":"Ewald","year":"1961","journal-title":"Small Anim. Clin."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/rda.12103","article-title":"Breed variations in the incidence of pyometra and mammary tumours in Swedish dogs","volume":"47","author":"Jitpean","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1111\/j.1939-1676.2001.tb01587.x","article-title":"Breed risk of pyometra in insured dogs in Sweden","volume":"15","author":"Egenvall","year":"2001","journal-title":"J. Vet. Intern. 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