{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T02:18:56Z","timestamp":1768616336587,"version":"3.49.0"},"reference-count":36,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T00:00:00Z","timestamp":1672012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pr\u00f3-Reitoria de Ensino de P\u00f3s-Gradua\u00e7\u00e3o e Pesquisa da Universidade Federal da Grande Dourados (PROPP-UFGD)","award":["FUNDECT: 275\/2016"],"award-info":[{"award-number":["FUNDECT: 275\/2016"]}]},{"name":"Pr\u00f3-Reitoria de Ensino de P\u00f3s-Gradua\u00e7\u00e3o e Pesquisa da Universidade Federal da Grande Dourados (PROPP-UFGD)","award":["313047\/2020-0"],"award-info":[{"award-number":["313047\/2020-0"]}]},{"name":"Pr\u00f3-Reitoria de Ensino de P\u00f3s-Gradua\u00e7\u00e3o e Pesquisa da Universidade Federal da Grande Dourados (PROPP-UFGD)","award":["24.073-\u00c2"],"award-info":[{"award-number":["24.073-\u00c2"]}]},{"name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","award":["FUNDECT: 275\/2016"],"award-info":[{"award-number":["FUNDECT: 275\/2016"]}]},{"name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","award":["313047\/2020-0"],"award-info":[{"award-number":["313047\/2020-0"]}]},{"name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","award":["24.073-\u00c2"],"award-info":[{"award-number":["24.073-\u00c2"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["FUNDECT: 275\/2016"],"award-info":[{"award-number":["FUNDECT: 275\/2016"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["313047\/2020-0"],"award-info":[{"award-number":["313047\/2020-0"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["24.073-\u00c2"],"award-info":[{"award-number":["24.073-\u00c2"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["FUNDECT: 275\/2016"],"award-info":[{"award-number":["FUNDECT: 275\/2016"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["313047\/2020-0"],"award-info":[{"award-number":["313047\/2020-0"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["24.073-\u00c2"],"award-info":[{"award-number":["24.073-\u00c2"]}]},{"name":"Financiadora de Estudos e Projetos (Finep)","award":["FUNDECT: 275\/2016"],"award-info":[{"award-number":["FUNDECT: 275\/2016"]}]},{"name":"Financiadora de Estudos e Projetos (Finep)","award":["313047\/2020-0"],"award-info":[{"award-number":["313047\/2020-0"]}]},{"name":"Financiadora de Estudos e Projetos (Finep)","award":["24.073-\u00c2"],"award-info":[{"award-number":["24.073-\u00c2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Melipona quadrifasciata anthidioides and Scaptotrigona depilis are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions and evaluate the antimicrobial activity of propolis produced by M. q. anthidioides and S. depilis. The ethanolic extracts of propolis of M. q. anthidioides (EEP-M) and S. depilis (EEP-S) were prepared, and their chemical constituents were characterized by HPLC-ESI-MS. The antimicrobial activity was evaluated against bacteria and fungi, isolated from reference strains and hospital origin resistant to the action of antibiotics. From EEP-M, phenolic compounds were annotated, including gallic acid, ellagic acid, and flavonoids, as well as diterpenes and triterpenes. EEP-S showed mainly triterpene in its chemical composition. Both extracts inhibited the growth of medically relevant bacteria and fungi, including hospital-acquired and antimicrobial-resistant. In general, EEP-S showed better antimicrobial activity compared to EEP-M. The MIC of EEP-S against vancomycin-resistant Enterococcus faecalis was 3.50 mg\/mL, while the MIC of EEP-M was 5.33 \u00b1 0.16 mg\/mL. In conclusion, this study shows that propolis produced by M. q. anthidioides and S. depilis has the potential to be used for the prevention or treatment of microbial infections.<\/jats:p>","DOI":"10.3390\/microorganisms11010068","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T07:30:06Z","timestamp":1672126206000},"page":"68","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antimicrobial Activity of Propolis from the Brazilian Stingless Bees Melipona quadrifasciata anthidioides and Scaptotrigona depilis (Hymenoptera, Apidae, Meliponini)"],"prefix":"10.3390","volume":"11","author":[{"given":"Jaqueline Ferreira","family":"Campos","sequence":"first","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thaliny","family":"Bonamigo","sequence":"additional","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1019-1202","authenticated-orcid":false,"given":"Paola dos Santos da","family":"Rocha","sequence":"additional","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7281-8598","authenticated-orcid":false,"given":"Vanessa Marina Branco","family":"Paula","sequence":"additional","affiliation":[{"name":"CIMO-Mountain Research Center, Department of Biology and Biotechnology, Polytechnic Institute of Bragan\u00e7a, Campus Santa Apol\u00f3nia, Agricultural College of Bragan\u00e7a, Bragan\u00e7a 5301-855, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uilson Pereira dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Benedito Perrella","family":"Balestieri","sequence":"additional","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0872-2756","authenticated-orcid":false,"given":"Denise Brentan","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universit\u00e1ria, Campo Grande 79070-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1231-9441","authenticated-orcid":false,"given":"Carlos Alexandre","family":"Carollo","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universit\u00e1ria, Campo Grande 79070-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia M.","family":"Estevinho","sequence":"additional","affiliation":[{"name":"CIMO-Mountain Research Center, Department of Biology and Biotechnology, Polytechnic Institute of Bragan\u00e7a, Campus Santa Apol\u00f3nia, Agricultural College of Bragan\u00e7a, Bragan\u00e7a 5301-855, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5764-2125","authenticated-orcid":false,"given":"Kely","family":"de Picoli Souza","sequence":"additional","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6557-7914","authenticated-orcid":false,"given":"Edson Lucas dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,26]]},"reference":[{"key":"ref_1","unstructured":"Moure, J.S., Urban, D., and Melo, G.A.R. 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