{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:44:33Z","timestamp":1760147073281,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>With the advent of omics technology and the improved culturing of anaerobic microbes, there is a good understanding of the microorganisms present in a healthy and diseased vagina. This has led to the identification of a select few Lactobacillus species associated with health. However, the origin of these species and how they reach the vagina remains unclear, as does their timing of colonization. In an effort to create badly needed therapies for women, these gaps in knowledge need to be addressed.<\/jats:p>","DOI":"10.3390\/microorganisms11010148","type":"journal-article","created":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T03:31:28Z","timestamp":1672975888000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["How Do Lactobacilli Search and Find the Vagina?"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9658-5696","authenticated-orcid":false,"given":"Gregor","family":"Reid","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Immunology, and Surgery, University of Western Ontario, London, ON N6A 3K7, Canada"},{"name":"Lawson Health Research Institute, London, ON N6A 4V2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1097\/MPG.0000000000000925","article-title":"Early gut colonization with lactobacilli and Staphylococcus in infants: The Hygiene Hypothesis extended","volume":"62","author":"Salminen","year":"2016","journal-title":"J. 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Gynecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1086\/339884","article-title":"Characterization of vaginal flora and bacterial vaginosis in women who have sex with women","volume":"185","author":"Marrazzo","year":"2002","journal-title":"J. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.ajog.2016.09.075","article-title":"Evidence that the endometrial microbiota has an effect on implantation success or failure","volume":"215","author":"Moreno","year":"2016","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/s41564-022-01083-2","article-title":"Towards a deeper understanding of the vaginal microbiota","volume":"7","author":"France","year":"2022","journal-title":"Nat. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s40168-019-0667-9","article-title":"Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: Implications for in vivo dominance of the vaginal microbiota","volume":"7","author":"Hertzberger","year":"2019","journal-title":"Microbiome"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1139\/w98-068","article-title":"Effect of nutrient composition on the in vitro growth of urogenital lactobacilli and uropathogens","volume":"44","author":"Reid","year":"1998","journal-title":"Can. J. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s40168-022-01400-1","article-title":"Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip","volume":"10","author":"Mahajan","year":"2022","journal-title":"Microbiome"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1001\/jama.287.15.1940","article-title":"Lactobacillus strains and vaginal ecology","volume":"287","author":"Cadieux","year":"2002","journal-title":"JAMA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"863208","DOI":"10.3389\/fcimb.2022.863208","article-title":"Clustering analysis of the multi-microbial consortium by Lactobacillus species against vaginal dysbiosis among Ecuadorian women","volume":"12","author":"Cueva","year":"2022","journal-title":"Front. Cell. Infect. 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