{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:45:39Z","timestamp":1760147139380,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671985","2022SKLID209","2019SKLID403","2100409034"],"award-info":[{"award-number":["81671985","2022SKLID209","2019SKLID403","2100409034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Foundation for the State Key Laboratory for Infectious Disease Prevention and Control of China","award":["81671985","2022SKLID209","2019SKLID403","2100409034"],"award-info":[{"award-number":["81671985","2022SKLID209","2019SKLID403","2100409034"]}]},{"name":"Public Health Service Capability Improvement Project of the National Health Commission of the People\u2019s Republic of China","award":["81671985","2022SKLID209","2019SKLID403","2100409034"],"award-info":[{"award-number":["81671985","2022SKLID209","2019SKLID403","2100409034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Legionella pneumophila is an intracellular pathogen causing pneumonia in humans. In February 2022, Legionnaires\u2019 disease caused by L. pneumophila strain Corby in a patient with lung adenocarcinoma was identified for the first time in China. This paper includes the case report and phenotypic and genomic analysis of the Corby (ICDC) strain. Its biological characteristics were evaluated by antibiotic sensitivity testing and cytology experiments, and genomic analysis was performed to understand its genetic evolution. The patient\u2019s clinical manifestations included cough, fever, pulmonary infiltration, and significantly decreased activity endurance. After empirical antimicrobial therapy, infection indicators decreased. The Corby (ICDC) strain was susceptible to nine antibiotics and exhibited strong intracellular proliferation ability. A phylogenetic tree showed that the Corby (ICDC) strain was closely related to the Corby strain, but under the pressure of a complex environment, its genome had undergone more rearrangement and inversion. The type IF CRISPR-Cas system was identified in its genome, and spacer analysis indicated that it had been invaded by several foreign plasmids, bacteria, and viruses during evolution. Legionnaires\u2019 disease caused by L. pneumophila strain Corby may be ignored in China, and it is urgent to improve long-term monitoring and investigation of aquatic environments and patients with respiratory infections to prevent a large-scale outbreak of Legionnaires\u2019 disease.<\/jats:p>","DOI":"10.3390\/microorganisms11010204","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T02:05:52Z","timestamp":1673575552000},"page":"204","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Legionnaires\u2019 Disease in China Caused by Legionella pneumophila Corby"],"prefix":"10.3390","volume":"11","author":[{"given":"Pei-Xing","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Yu","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Peking University People\u2019s Hospital, Beijing 100044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Jing","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8495-9221","authenticated-orcid":false,"given":"Zhan-Cheng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Peking University People\u2019s Hospital, Beijing 100044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1146\/annurev-pathmechdis-012419-032742","article-title":"Legionnaires\u2019 Disease: State of the Art Knowledge of Pathogenesis Mechanisms of Legionella","volume":"15","author":"Mondino","year":"2020","journal-title":"Annu. 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