{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:17:18Z","timestamp":1767651438885,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut Pasteur Labex IBEID","award":["ANR-10-LABX-62-IBEID","COST Action TD1303","POSDRU\/159\/1.5\/S\/132765"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID","COST Action TD1303","POSDRU\/159\/1.5\/S\/132765"]}]},{"DOI":"10.13039\/501100006488","name":"INRA","doi-asserted-by":"publisher","award":["ANR-10-LABX-62-IBEID","COST Action TD1303","POSDRU\/159\/1.5\/S\/132765"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID","COST Action TD1303","POSDRU\/159\/1.5\/S\/132765"]}],"id":[{"id":"10.13039\/501100006488","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Social Fund Program, Human Resources Development Operation 2007\u20132013","award":["ANR-10-LABX-62-IBEID","COST Action TD1303","POSDRU\/159\/1.5\/S\/132765"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID","COST Action TD1303","POSDRU\/159\/1.5\/S\/132765"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Tick-borne diseases are responsible for many vector-borne diseases within Europe. Recently, novel viruses belonging to a new viral family of the order Bunyavirales were discovered in numerous tick species. In this study, we used metatranscriptomics to detect the virome, including novel viruses, associated with Ixodes ricinus collected from Romania and France. A bunyavirus-like virus related to the Bronnoya virus was identified for the first time in these regions. It presents a high level of amino-acid conservation with Bronnoya-related viruses identified in I. ricinus ticks from Norway and Croatia and with the Ixodes scapularis bunyavirus isolated from a tick cell line in Japan in 2014. Phylogenetic analyses revealed that the Bronnoya viruses\u2019 sub-clade is distinct from several Bunyavirales families, suggesting that it could constitute a novel family within the order. To determine if Bronnoya viruses could constitute novel tick-borne arboviruses, a Luciferase immunoprecipitation assay for detecting antibodies in the viral glycoprotein of the Romanian Bronnoya virus was used to screen sera from small ruminants exposed to tick bites. No positive serum was detected, suggesting that this virus is probably not able to infect small ruminants. This study represents the first serological investigation of mammalian infections with a Bronnoya-like virus and an initial step in the identification of potential new emergences of tick-borne arboviruses.<\/jats:p>","DOI":"10.3390\/microorganisms11010209","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T03:10:34Z","timestamp":1673838634000},"page":"209","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Search for Tick-Associated, Bronnoya-like Virus Spillover into Sheep"],"prefix":"10.3390","volume":"11","author":[{"given":"Bianca Elena","family":"Bratuleanu","sequence":"first","affiliation":[{"name":"Pathogen Discovery Laboratory, Institut Pasteur, 75015 Paris, France"},{"name":"Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety (ROVETEMERG), \u201cIon Ionescu de la Brad\u201d, Iasi University of Life Sciences, 700490 Ia\u0219i, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian","family":"Raileanu","sequence":"additional","affiliation":[{"name":"Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety (ROVETEMERG), \u201cIon Ionescu de la Brad\u201d, Iasi University of Life Sciences, 700490 Ia\u0219i, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"Chr\u00e9tien","sequence":"additional","affiliation":[{"name":"Pathogen Discovery Laboratory, Institut Pasteur, 75015 Paris, France"},{"name":"WOAH (OIE) Collaborating Centre for Detection and Identification in Humans of Emerging Animal Pathogens, Institut Pasteur, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7292-5270","authenticated-orcid":false,"given":"Pablo","family":"Guardado-Calvo","sequence":"additional","affiliation":[{"name":"Department of Virology, Structural Virology Unit, CNRS UMR 3569, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8504-4675","authenticated-orcid":false,"given":"Thomas","family":"Bigot","sequence":"additional","affiliation":[{"name":"Pathogen Discovery Laboratory, Institut Pasteur, 75015 Paris, France"},{"name":"Bioinformatics and Biostatistics Hub, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gheorghe","family":"Savuta","sequence":"additional","affiliation":[{"name":"Regional Center of Advanced Research for Emerging Diseases, Zoonoses and Food Safety (ROVETEMERG), \u201cIon Ionescu de la Brad\u201d, Iasi University of Life Sciences, 700490 Ia\u0219i, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3655-9220","authenticated-orcid":false,"given":"Sarah","family":"Temmam","sequence":"additional","affiliation":[{"name":"Pathogen Discovery Laboratory, Institut Pasteur, 75015 Paris, France"},{"name":"WOAH (OIE) Collaborating Centre for Detection and Identification in Humans of Emerging Animal Pathogens, Institut Pasteur, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1853-7207","authenticated-orcid":false,"given":"Marc","family":"Eloit","sequence":"additional","affiliation":[{"name":"Pathogen Discovery Laboratory, Institut Pasteur, 75015 Paris, France"},{"name":"WOAH (OIE) Collaborating Centre for Detection and Identification in Humans of Emerging Animal Pathogens, Institut Pasteur, 75015 Paris, France"},{"name":"Department of Biological and Pharmaceutical Sciences, Alfort National Veterinary School, 94700 Maisons-Alfort, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1017\/S0031182004005967","article-title":"The global importance of ticks","volume":"129","author":"Jongejan","year":"2004","journal-title":"Parasitology"},{"key":"ref_2","first-page":"1040","article-title":"Tick-borne virus diseases of human interest in Europe","volume":"10","author":"Charrel","year":"2004","journal-title":"Clin. 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