{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:47:17Z","timestamp":1760147237878,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s (FAPEG)"},{"name":"National Institute of Science and Technology of strategies of host\u2013pathogen interaction (INCT-IPH)"},{"name":"Conselho Nacional de Desenvolvimmento Cient\u00edfico e Tecnol\u00f3gico (CNPq)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Paracoccidioides spp. are endemic fungi from Latin America that cause Paracoccidioidomycosis, a systemic disease. These fungi present systems for high-affinity metal uptake, storage, and mobilization, which counteract host nutritional immunity and mitigate the toxic effects of metals. Regarding Cu mobilization, the metallochaperone Atx1 is regulated according to Cu bioavailability in Paracoccidioides spp., contributing to metal homeostasis. However, additional information in the literature on PbAtx1 is scarce. Therefore, in the present work, we aimed to study the PbAtx1 protein\u2013protein interaction networks. Heterologous expressed PbAtx1 was used in a pull-down assay with Paracoccidioides brasiliensis cytoplasmic extract. Nineteen proteins that interacted with PbAtx1 were identified by HPLC-MSE. Among them, a relevant finding was a Cytochrome b5 (PbCyb5), regulated by Fe bioavailability in Aspergillus fumigatus and highly secreted by P. brasiliensis in Fe deprivation. We validated the interaction between PbAtx1-PbCyb5 through molecular modeling and far-Western analyses. It is known that there is a relationship between Fe homeostasis and Cu homeostasis in organisms. In this sense, would PbAtx1-PbCyb5 interaction be a new metal-sensor system? Would it be supported by the presence\/absence of metals? We intend to answer those questions in future works to contribute to the understanding of the strategies employed by Paracoccidioides spp. to overcome host defenses.<\/jats:p>","DOI":"10.3390\/microorganisms11020248","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T03:16:52Z","timestamp":1674098212000},"page":"248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Interactions of the Copper Chaperone Atx1 of Paracoccidioides brasiliensis with Fungal Proteins Suggest a Crosstalk between Iron and Copper Homeostasis"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1513-3247","authenticated-orcid":false,"given":"Marcos Antonio Batista","family":"de Carvalho J\u00fanior","sequence":"first","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1655-9576","authenticated-orcid":false,"given":"Lana O\u2019Hara Souza","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurine Lacerda","family":"Pigosso","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8661-4086","authenticated-orcid":false,"given":"Aparecido Ferreira","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danize Eukales Menezes","family":"Lugo","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1031-1465","authenticated-orcid":false,"given":"Dayane","family":"Moraes","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5350-7313","authenticated-orcid":false,"given":"Kleber Santiago","family":"Freitas e Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-8036","authenticated-orcid":false,"given":"Maristela","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lia Maria de Almeida","family":"Soares","sequence":"additional","affiliation":[{"name":"Laboratorio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 70690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/yea.3147","article-title":"New Insights into a Complex Fungal Pathogen: The Case of Paracoccidioides spp.","volume":"33","author":"Gonzalez","year":"2015","journal-title":"Yeast"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3389\/fcimb.2013.00080","article-title":"Shared and Distinct Mechanisms of Iron Acquisition by Bacterial and Fungal Pathogens of Humans","volume":"3","author":"Caza","year":"2013","journal-title":"Front. 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