{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T01:44:33Z","timestamp":1783388673163,"version":"3.54.6"},"reference-count":43,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,22]],"date-time":"2023-01-22T00:00:00Z","timestamp":1674345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"KAKENHI, Japan","doi-asserted-by":"publisher","award":["AMED 17940819"],"award-info":[{"award-number":["AMED 17940819"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The nanomachine referred to as the type III secretion system (T3SS) is used by many Gram-negative pathogens or symbionts to inject their effector proteins into host cells to promote their infections or symbioses. Among the genera possessing T3SS is Vibrio, which consists of diverse species of Gammaproteobacteria including human pathogenic species and inhabits aquatic environments. We describe the genetic overview of the T3SS gene clusters in Vibrio through a phylogenetic analysis from 48 bacterial strains and a gene order analysis of the two previously known categories in Vibrio (T3SS1 and T3SS2). Through this analysis we identified a new T3SS category (named T3SS3) that shares similar core and related proteins (effectors, translocons, and chaperones) with the Ssa-Esc family of T3SSs in Salmonella, Shewanella, and Sodalis. The high similarity between T3SS3 and the Ssa-Esc family suggests a possibility of genetic exchange among marine bacteria with similar habitats.<\/jats:p>","DOI":"10.3390\/microorganisms11020290","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T03:21:54Z","timestamp":1674616914000},"page":"290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome Analysis Identifies a Novel Type III Secretion System (T3SS) Category in Vibrio Species"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8872-8132","authenticated-orcid":false,"given":"Douaa","family":"Zakaria","sequence":"first","affiliation":[{"name":"Department of Genetics, SOKENDAI University, Mishima 411-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeaki","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Iida","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6706-0487","authenticated-orcid":false,"given":"Masanori","family":"Arita","sequence":"additional","affiliation":[{"name":"Department of Genetics, SOKENDAI University, Mishima 411-8540, Japan"},{"name":"RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.tim.2016.09.008","article-title":"Non-cholera vibrios: The microbial barometer of climate change","volume":"25","author":"Trinanes","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1146\/annurev-micro-092412-155725","article-title":"Bacterial type III secretion systems: Specialized nanomachines for protein delivery into target cells","volume":"68","author":"Marlovits","year":"2014","journal-title":"Ann. 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