{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:10:06Z","timestamp":1792789806258,"version":"4.5.0"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["RC IZS SI 09\/15"],"award-info":[{"award-number":["RC IZS SI 09\/15"]}]},{"name":"Italian Ministry of Health","award":["RC IZS SI 11\/16"],"award-info":[{"award-number":["RC IZS SI 11\/16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Leptospirosis is a zoonosis of public health concern. Its prevalence in stray animals in the South of Italy is unknown. This study aimed to investigate Leptospira spp. prevalence in 1009 stray animals. Out of them, 749 were alive animals, including 358 dogs (316 from Palermo and 42 from Ragusa) and 391 cats (359 from Palermo and 32 from Ragusa), and 260 were corpses (216 dogs and 44 cats) randomly collected in Sicily. Dogs and cats underwent a serological screening by Microscopic Agglutination Test and a molecular investigation by Real-Time PCR targeting lipL32. Corpses were subjected to Real-Time PCR. Serological analyses showed a prevalence of 1.12% (4\/358) for dogs and 0.26% (1\/391) for cats, with the only positive cat coming from Palermo. Serogroup Icterohaemorrhagiae serovar Icterohaemorrhagiae or Copenhageni, followed by Canicola and Bratislava, were the most spread among dogs, while the serological positive cat reacted with Hardjo serogroup. Two urine (2\/32, 6.25%) and one blood (1\/391, 0.26%) samples of cats, all from Ragusa, were positive at Real-Time PCR for pathogenic Leptospira spp. Sequencing analyses showed the presence of L. interrogans serogroup Icterohaemorrhagiae serovar Icterohaemorrhagiae or Copenhageni in one of the positive urine samples and in the positive blood sample. Analyses on corpses showed a prevalence of 1.85% (4\/216) in Sicilian dog kidney samples, while all corpses of cats resulted in negative. Genotyping analysis showed a genetic relatedness between cat and human isolates. Results show Leptospira spp. circulation among Sicilian stray animals. The genetic relatedness between cat and human isolates suggests a possible common infection source.<\/jats:p>","DOI":"10.3390\/microorganisms11020385","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T02:35:10Z","timestamp":1675391710000},"page":"385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Serological and Molecular Evidence of Pathogenic Leptospira spp. in Stray Dogs and Cats of Sicily (South Italy), 2017\u20132021"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1470-6496","authenticated-orcid":false,"given":"Francesca","family":"Grippi","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5768-5782","authenticated-orcid":false,"given":"Vincenza","family":"Cannella","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0296-8846","authenticated-orcid":false,"given":"Giusi","family":"Macaluso","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4657-407X","authenticated-orcid":false,"given":"Valeria","family":"Blanda","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. 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Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1766-5327","authenticated-orcid":false,"given":"Paola","family":"Galluzzo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Sciacca","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalia","family":"D\u2019Agostino","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. 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