{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T08:48:54Z","timestamp":1782636534802,"version":"3.54.5"},"reference-count":53,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T00:00:00Z","timestamp":1677196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Molecular Biology Laboratory of the Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente de la Universidad Aut\u00f3noma de Tlaxcala"},{"name":"Instituto Nacional de Pediatr\u00eda, Mexico City, Mexico"},{"name":"INP 038\/2019"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Laccases are valuable enzymes as an excellent ecological alternative for bioremediation issues because they can oxidize persistent xenobiotic compounds. The production and characterization of extracellular laccases from saprotrophic fungi from disturbed environments have been scarcely explored, even though this could diversify their functional characteristics and expand the conditions in which they carry out their catalysis. Agrocybe pediades, isolated from a disturbed forest, produces an extracellular laccase in liquid culture. The enzyme was purified, identified and characterized. Copper and hexachlorobenzene do not function as inducers for the laccase produced. Partial amino acid sequences were obtained by LC-MS\/MS that share similarity with laccases from other fungi. Purified laccase is a monomer with a molecular mass between 55\u201360 kDa and had an optimum activity at pH 5.0 and the optimum temperature at 45 \u00b0C using 2,6-dimethoxyphenol (2,6-DMP) as substrate. The Km and Vmax also determined with 2,6-DMP were 100 \u03bcM and 285 \u03bcmol\u2219min\u22121\u2219mg\u22121, respectively, showing that the laccase of A. pediades has a higher affinity for this substrate than that of other Agaricales. These features could provide a potential catalyst for different toxic substrates and in the future laccase could be used in environmental recovery processes.<\/jats:p>","DOI":"10.3390\/microorganisms11030568","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T03:01:01Z","timestamp":1677207661000},"page":"568","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Laccase Production from Agrocybe pediades: Purification and Functional Characterization of a Consistent Laccase Isoenzyme in Liquid Culture"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5887-6193","authenticated-orcid":false,"given":"Paulina","family":"Gonz\u00e1lez-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Maestr\u00eda en Ciencias en Sistemas del Ambiente, Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2095-9182","authenticated-orcid":false,"given":"Sa\u00fal","family":"G\u00f3mez-Manzo","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Gen\u00e9tica, Instituto Nacional de Pediatr\u00eda, Secretar\u00eda de Salud, Ciudad de Mexico 04530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araceli","family":"Tomasini","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, CBS, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Ciudad de Mexico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Mart\u00ednez y P\u00e9rez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6187-0208","authenticated-orcid":false,"given":"Edelmira","family":"Garc\u00eda Nieto","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7965-8416","authenticated-orcid":false,"given":"Arely","family":"Anaya-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvia","family":"Ortiz Ortiz","sequence":"additional","affiliation":[{"name":"Facultad de Odontolog\u00eda, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4117-6689","authenticated-orcid":false,"given":"Rosa Ang\u00e9lica","family":"Castillo Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"CICATA Unidad Morelos, Instituto Polit\u00e9cnico Nacional. Boulevard de la Tecnolog\u00eda, 1036 Z-1, P 2\/2, Atlacholoaya 62790, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4591-7379","authenticated-orcid":false,"given":"Jaime","family":"Marcial-Quino","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba M\u00f3nica","family":"Montiel-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Gen\u00e9tica y Ambiente, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Eichlerov\u00e1, I., and Baldrian, P. (2020). 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