{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T06:53:20Z","timestamp":1777100000824,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T00:00:00Z","timestamp":1678060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001843","name":"SERB","doi-asserted-by":"publisher","award":["EMR\/2016\/007845"],"award-info":[{"award-number":["EMR\/2016\/007845"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"SERB","doi-asserted-by":"publisher","award":["BT\/PR14240\/MED\/29\/985\/2015"],"award-info":[{"award-number":["BT\/PR14240\/MED\/29\/985\/2015"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"SERB","doi-asserted-by":"publisher","award":["STARS\/APR2019\/BS\/584\/FS"],"award-info":[{"award-number":["STARS\/APR2019\/BS\/584\/FS"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"DBT","doi-asserted-by":"publisher","award":["EMR\/2016\/007845"],"award-info":[{"award-number":["EMR\/2016\/007845"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"DBT","doi-asserted-by":"publisher","award":["BT\/PR14240\/MED\/29\/985\/2015"],"award-info":[{"award-number":["BT\/PR14240\/MED\/29\/985\/2015"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"DBT","doi-asserted-by":"publisher","award":["STARS\/APR2019\/BS\/584\/FS"],"award-info":[{"award-number":["STARS\/APR2019\/BS\/584\/FS"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MoE-IISc-STARS","award":["EMR\/2016\/007845"],"award-info":[{"award-number":["EMR\/2016\/007845"]}]},{"name":"MoE-IISc-STARS","award":["BT\/PR14240\/MED\/29\/985\/2015"],"award-info":[{"award-number":["BT\/PR14240\/MED\/29\/985\/2015"]}]},{"name":"MoE-IISc-STARS","award":["STARS\/APR2019\/BS\/584\/FS"],"award-info":[{"award-number":["STARS\/APR2019\/BS\/584\/FS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Dengue virus infections are recorded as hyper-endemic in many countries, including India. Research pertaining to the reasons for frequent outbreaks and severe dengue is ongoing. Hyderabad city, India, has been recorded as a \u2018hotspot\u2019 for dengue virus infections. Dengue virus strains circulating over the past few years in Hyderabad city have been characterized at the molecular level to analyze the serotype\/genotypes; 3\u2032UTRs were further amplified and sequenced. The disease severity in patients infected with dengue virus strains with complete and 3\u2032UTR deletion mutants was analyzed. Genotype I of the serotype 1 replaced genotype III, which has been circulating over the past few years in this region. Coincidentally, the number of dengue virus infections significantly increased in this region during the study period. Nucleotide sequence analysis suggested twenty-two and eight nucleotide deletions in the 3\u2032UTR of DENV-1. The eight nucleotide deletions observed in the case of DENV-1 3\u2032UTR were the first reported in this instance. A 50 nucleotide deletion was identified in the case of the serotype DENV-2. Importantly, these deletion mutants were found to cause severe dengue, even though they were found to be replication incompetent. This study emphasized the role of dengue virus 3\u2032UTRs on severe dengue and emerging outbreaks.<\/jats:p>","DOI":"10.3390\/microorganisms11030666","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T06:29:06Z","timestamp":1678084146000},"page":"666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Association of Dengue Virus Serotypes 1&amp;2 with Severe Dengue Having Deletions in Their 3\u2032Untranslated Regions (3\u2032UTRs)"],"prefix":"10.3390","volume":"11","author":[{"given":"Deepti","family":"Maisnam","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad 500046, Telangana, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arcy","family":"Billoria","sequence":"additional","affiliation":[{"name":"Lotus Children Hospital, Lakdikapul, Hyderabad 500004, Telangana, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. S. V","family":"Prasad","sequence":"additional","affiliation":[{"name":"Lotus Children Hospital, Lakdikapul, Hyderabad 500004, Telangana, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musturi","family":"Venkataramana","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad 500046, Telangana, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1179\/2046904712Z.00000000052","article-title":"The revised WHO dengue case classification: Does the system need to be modified?","volume":"32","author":"Hadinegoro","year":"2012","journal-title":"Paediatr. 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