{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:05:42Z","timestamp":1790795142529,"version":"4.1.0"},"reference-count":89,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Funds GURDTI","award":["2018-1828-0002370"],"award-info":[{"award-number":["2018-1828-0002370"]}]},{"name":"European Regional Development Funds GURDTI","award":["DIRED\/20191765"],"award-info":[{"award-number":["DIRED\/20191765"]}]},{"name":"R\u00e9gion R\u00e9union","award":["2018-1828-0002370"],"award-info":[{"award-number":["2018-1828-0002370"]}]},{"name":"R\u00e9gion R\u00e9union","award":["DIRED\/20191765"],"award-info":[{"award-number":["DIRED\/20191765"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Despite considerable advances in medicine and technology, humanity still faces many deadly diseases such as cancer and malaria. In order to find appropriate treatments, the discovery of new bioactive substances is essential. Therefore, research is now turning to less frequently explored habitats with exceptional biodiversity such as the marine environment. Many studies have demonstrated the therapeutic potential of bioactive compounds from marine macro- and microorganisms. In this study, nine microbial strains isolated from an Indian Ocean sponge, Scopalina hapalia, were screened for their chemical potential. The isolates belong to different phyla, some of which are already known for their production of secondary metabolites, such as the actinobacteria. This article aims at describing the selection method used to identify the most promising microorganisms in the field of active metabolites production. The method is based on the combination of their biological and chemical screening, coupled with the use of bioinformatic tools. The dereplication of microbial extracts and the creation of a molecular network revealed the presence of known bioactive molecules such as staurosporin, erythromycin and chaetoglobosins. Molecular network exploration indicated the possible presence of novel compounds in clusters of interest. The biological activities targeted in the study were cytotoxicity against the HCT-116 and MDA-MB-231 cell lines and antiplasmodial activity against Plasmodium falciparum 3D7. Chaetomium globosum SH-123 and Salinispora arenicola SH-78 strains actually showed remarkable cytotoxic and antiplasmodial activities, while Micromonospora fluostatini SH-82 demonstrated promising antiplasmodial effects. The ranking of the microorganisms as a result of the different screening steps allowed the selection of a promising strain, Micromonospora fluostatini SH-82, as a premium candidate for the discovery of new drugs.<\/jats:p>","DOI":"10.3390\/microorganisms11030697","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T02:33:16Z","timestamp":1678329196000},"page":"697","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Prioritization of Microorganisms Isolated from the Indian Ocean Sponge Scopalina hapalia Based on Metabolomic Diversity and Biological Activity for the Discovery of Natural Products"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4799-1137","authenticated-orcid":false,"given":"Alexandre","family":"Le Loarer","sequence":"first","affiliation":[{"name":"Laboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La R\u00e9union, 15 Avenue Ren\u00e9 Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5114-304X","authenticated-orcid":false,"given":"R\u00e9my","family":"Marcellin-Gros","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"},{"name":"School of Pharmaceutical Sciences, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7392-355X","authenticated-orcid":false,"given":"Laurent","family":"Dufoss\u00e9","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La R\u00e9union, 15 Avenue Ren\u00e9 Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6294-9671","authenticated-orcid":false,"given":"J\u00e9r\u00f4me","family":"Bignon","sequence":"additional","affiliation":[{"name":"Institute of Chemistry of Natural Substances (ICSN), CNRS UPR 2301, Universit\u00e9 Paris-Saclay, 1, av. de la Terrasse, CEDEX, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0770-9990","authenticated-orcid":false,"given":"Michel","family":"Fr\u00e9d\u00e9rich","sequence":"additional","affiliation":[{"name":"Pharmacognosy Laboratory, Department of Pharmacy, Centre Interfacultaire de Recherche sur le M\u00e9dicament (CIRM), University of Li\u00e8ge, Quartier H\u00f4pital, Avenue Hippocrate 15, Bat. B36, Campus du Sart-Tilman, B-4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4052-6336","authenticated-orcid":false,"given":"Allison","family":"Ledoux","sequence":"additional","affiliation":[{"name":"Pharmacognosy Laboratory, Department of Pharmacy, Centre Interfacultaire de Recherche sur le M\u00e9dicament (CIRM), University of Li\u00e8ge, Quartier H\u00f4pital, Avenue Hippocrate 15, Bat. B36, Campus du Sart-Tilman, B-4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9567-1664","authenticated-orcid":false,"given":"Emerson Ferreira","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"},{"name":"School of Pharmaceutical Sciences, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0125-952X","authenticated-orcid":false,"given":"Jean-Luc","family":"Wolfender","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"},{"name":"School of Pharmaceutical Sciences, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5188-0704","authenticated-orcid":false,"given":"Anne","family":"Gauvin-Bialecki","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La R\u00e9union, 15 Avenue Ren\u00e9 Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0562-2154","authenticated-orcid":false,"given":"Mireille","family":"Fouillaud","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La R\u00e9union, 15 Avenue Ren\u00e9 Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"ref_1","unstructured":"(2022, November 16). World Health Organization Website. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/the-top-10-causes-of-death."},{"key":"ref_2","unstructured":"(2022, November 16). World Health Organization Website. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cancer."},{"key":"ref_3","unstructured":"(2022, November 16). World Health Organization Website. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cardiovascular-diseases-(cvds)."},{"key":"ref_4","unstructured":"IARC (1976). IARC Biennial Report 2020-2021, IARC Scientific Publications."},{"key":"ref_5","unstructured":"(2021). World Health Organization World Malaria Report 2021, World Health Organization."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1146\/annurev.biochem.78.082907.145923","article-title":"Multidrug Resistance in Bacteria","volume":"78","author":"Nikaido","year":"2009","journal-title":"Annu. Rev. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e51034","DOI":"10.15252\/embr.202051034","article-title":"Antibiotic Resistance and Persistence-Implications for Human Health and Treatment Perspectives","volume":"21","author":"Huemer","year":"2020","journal-title":"EMBO Rep."},{"key":"ref_8","unstructured":"(2022, November 16). Global Cancer Observatory, WHO Website. Available online: https:\/\/gco.iarc.fr\/tomorrow\/en."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural Products as Sources of New Drugs over the Nearly Four Decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. Nat. Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s10295-015-1723-5","article-title":"Natural Product Discovery: Past, Present, and Future","volume":"43","author":"Katz","year":"2016","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Khalifa, S.A.M., Elias, N., Farag, M.A., Chen, L., Saeed, A., Hegazy, M.-E.F., Moustafa, M.S., Abd El-Wahed, A., Al-Mousawi, S.M., and Musharraf, S.G. (2019). Marine Natural Products: A Source of Novel Anticancer Drugs. Mar. Drugs, 17.","DOI":"10.3390\/md17090491"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"116058","DOI":"10.1016\/j.bmc.2021.116058","article-title":"Application of Marine Natural Products in Drug Research","volume":"35","author":"Lu","year":"2021","journal-title":"Bioorganic Med. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1017\/S0025315415002106","article-title":"The marine biodiscovery pipeline and ocean medicines of tomorrow","volume":"96","author":"Jaspars","year":"2016","journal-title":"J. Mar. Biol. Assoc. U. K."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/S1359-6446(03)02713-2","article-title":"Drugs from the Deep: Marine Natural Products as Drug Candidates","volume":"8","author":"Haefner","year":"2003","journal-title":"Drug Discov. Today"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Taufa, T., Subramani, R., Northcote, P.T., and Keyzers, R.A. (2021). Natural Products from Tongan Marine Organisms. Molecules, 26.","DOI":"10.3390\/molecules26154534"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bai, X., Liu, Y., Wang, H., and Zhang, H. (2021). Natural Products from the Marine Sponge Subgenus Reniera. Molecules, 26.","DOI":"10.3390\/molecules26041097"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12983-015-0099-8","article-title":"Proposal for a Revised Classification of the Demospongiae (Porifera)","volume":"12","author":"Morrow","year":"2015","journal-title":"Front. Zool."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Helber, S., de Voogd, N., Muhando, C., Rohde, S., and Schupp, P. (2017). Anti-Predatory Effects of Organic Extracts of 10 Common Reef Sponges from Zanzibar. Hydrobiologia, 790.","DOI":"10.1007\/s10750-016-3036-8"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sa\u00efd Hassane, C., Herbette, G., Garayev, E., Mabrouki, F., Clerc, P., de Voogd, N.J., Greff, S., Trougakos, I.P., Ouazzani, J., and Fouillaud, M. (2022). New Metabolites from the Marine Sponge Scopalina hapalia Collected in Mayotte Lagoon. Mar. Drugs, 20.","DOI":"10.3390\/md20030186"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"fiz026","DOI":"10.1093\/femsec\/fiz026","article-title":"Sponges from Zanzibar Host Diverse Prokaryotic Communities with Potential for Natural Product Synthesis","volume":"95","author":"Helber","year":"2019","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e0197617","DOI":"10.1371\/journal.pone.0197617","article-title":"Sponge Chemical Defenses Are a Possible Mechanism for Increasing Sponge Abundance on Reefs in Zanzibar","volume":"13","author":"Helber","year":"2018","journal-title":"PLoS ONE"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ramesh, C., Tulasi, B.R., Raju, M., Thakur, N., and Dufoss\u00e9, L. (2021). Marine Natural Products from Tunicates and Their Associated Microbes. Mar. Drugs, 19.","DOI":"10.3390\/md19060308"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chen, L., Wang, X.-N., Bi, H.-Y., and Wang, G.-Y. (2022). Antimicrobial Biosynthetic Potential and Phylogenetic Analysis of Culturable Bacteria Associated with the Sponge Ophlitaspongia sp. from the Yellow Sea, China. Mar. Drugs, 20.","DOI":"10.3390\/md20100588"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Said Hassane, C., Fouillaud, M., Le Goff, G., Sklirou, A.D., Boyer, J.B., Trougakos, I.P., Jerabek, M., Bignon, J., de Voogd, N.J., and Ouazzani, J. (2020). Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process. Microorganisms, 8.","DOI":"10.3390\/microorganisms8091262"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e0138528","DOI":"10.1371\/journal.pone.0138528","article-title":"Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges","volume":"10","author":"Cheng","year":"2015","journal-title":"PLoS ONE"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"727","DOI":"10.3389\/fmicb.2019.00727","article-title":"Bioactivities and Extract Dereplication of Actinomycetales Isolated from Marine Sponges","volume":"10","author":"Santos","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Siro, G., Pipite, A., Christi, K., Srinivasan, S., and Subramani, R. (2022). Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites. Microorganisms, 10.","DOI":"10.3390\/microorganisms10071349"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7365","DOI":"10.1007\/s00253-014-5954-6","article-title":"Marine Actinobacteria Associated with Marine Organisms and Their Potentials in Producing Pharmaceutical Natural Products","volume":"98","author":"Valliappan","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"126708","DOI":"10.1016\/j.micres.2021.126708","article-title":"Actinobacteria in Natural Products Research: Progress and Prospects","volume":"246","author":"Jose","year":"2021","journal-title":"Microbiol. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Al-shaibani, M.M., Radin Mohamed, R.M.S., Sidik, N.M., Enshasy, H.A.E., Al-Gheethi, A., Noman, E., Al-Mekhlafi, N.A., and Zin, N.M. (2021). Biodiversity of Secondary Metabolites Compounds Isolated from Phylum Actinobacteria and Its Therapeutic Applications. Molecules, 26.","DOI":"10.3390\/molecules26154504"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.3390\/md12073929","article-title":"Marine Microorganism-Invertebrate Assemblages: Perspectives to Solve the \u201cSupply Problem\u201d in the Initial Steps of Drug Discovery","volume":"12","author":"Leal","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","article-title":"Natural Products in Drug Discovery: Advances and Opportunities","volume":"20","author":"Atanasov","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.tips.2019.11.002","article-title":"Targeting Bacterial Genomes for Natural Product Discovery","volume":"41","author":"Kalkreuter","year":"2020","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"16567","DOI":"10.1074\/jbc.REV119.006514","article-title":"Leveraging a Large Microbial Strain Collection for Natural Product Discovery","volume":"294","author":"Steele","year":"2019","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/s41589-019-0400-9","article-title":"A Computational Framework to Explore Large-Scale Biosynthetic Diversity","volume":"16","author":"Mullowney","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e02104-16","DOI":"10.1128\/mBio.02104-16","article-title":"Strain Prioritization and Genome Mining for Enediyne Natural Products","volume":"7","author":"Yan","year":"2016","journal-title":"mBio"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1021\/np5006168","article-title":"Strain Prioritization for Natural Product Discovery by a High-Throughput Real-Time PCR Method","volume":"77","author":"Hindra","year":"2014","journal-title":"J. Nat. Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e2000171","DOI":"10.1002\/minf.202000171","article-title":"Cheminformatics in Natural Product-Based Drug Discovery","volume":"39","author":"Chen","year":"2020","journal-title":"Mol. Inform."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3416","DOI":"10.3390\/md12063416","article-title":"Metabolomic Tools for Secondary Metabolite Discovery from Marine Microbial Symbionts","volume":"12","author":"Macintyre","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1177\/0192623307310947","article-title":"Metabolic Profiling as a Tool for Understanding Mechanisms of Toxicity","volume":"36","author":"Clarke","year":"2008","journal-title":"Toxicol. Pathol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4277","DOI":"10.1021\/ac202623g","article-title":"Microbial Strain Prioritization Using Metabolomics Tools for the Discovery of Natural Products","volume":"84","author":"Hou","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Soldatou, S., Eldj\u00e1rn, G.H., Ramsay, A., van der Hooft, J.J.J., Hughes, A.H., Rogers, S., and Duncan, K.R. (2021). Comparative Metabologenomics Analysis of Polar Actinomycetes. Mar. Drugs, 19.","DOI":"10.3390\/md19020103"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1093\/femsre\/fuy028","article-title":"Secondary Metabolite Production and the Safety of Industrially Important Members of the Bacillus subtilis Group","volume":"42","author":"Harwood","year":"2018","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.3390\/md10051092","article-title":"Structure Elucidation and Anticancer Activity of 7-Oxostaurosporine Derivatives from the Brazilian Endemic Tunicate Eudistoma vannamei","volume":"10","author":"Jimenez","year":"2012","journal-title":"Mar. Drugs"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"298","DOI":"10.2174\/1871520614666141114202606","article-title":"Marizomib, A Potent Second Generation Proteasome Inhibitor from Natural Origin","volume":"15","author":"Ma","year":"2015","journal-title":"ACAMC"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1128\/AAC.01825-12","article-title":"Chemobiosynthesis of New Antimalarial Macrolides","volume":"57","author":"Goodman","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3832","DOI":"10.1038\/s41467-021-23953-9","article-title":"Ion Identity Molecular Networking for Mass Spectrometry-Based Metabolomics in the GNPS Environment","volume":"12","author":"Schmid","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nbt.3597","article-title":"Sharing and Community Curation of Mass Spectrometry Data with Global Natural Products Social Molecular Networking","volume":"34","author":"Wang","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/s41592-019-0344-8","article-title":"SIRIUS 4: A Rapid Tool for Turning Tandem Mass Spectra into Metabolite Structure Information","volume":"16","author":"Fleischauer","year":"2019","journal-title":"Nat. Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"12580","DOI":"10.1073\/pnas.1509788112","article-title":"Searching Molecular Structure Databases with Tandem Mass Spectra Using CSI: FingerID","volume":"112","author":"Shen","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1021\/acs.analchem.5b04804","article-title":"Integration of Molecular Networking and In-Silico MS\/MS Fragmentation for Natural Products Dereplication","volume":"88","author":"Allard","year":"2016","journal-title":"Anal. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.3389\/fpls.2019.01329","article-title":"Taxonomically Informed Scoring Enhances Confidence in Natural Products Annotation","volume":"10","author":"Rutz","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1038\/nbt.2377","article-title":"A Cross-Platform Toolkit for Mass Spectrometry and Proteomics","volume":"30","author":"Chambers","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Pluskal, T., Castillo, S., Villar-Briones, A., and Ore\u0161i\u010d, M. (2010). mZmine 2: Modular Framework for Processing, Visualizing, and Analyzing Mass Spectrometry-Based Molecular Profile Data. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-395"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"8696","DOI":"10.1021\/acs.analchem.7b00947","article-title":"One Step Forward for Reducing False Positive and False Negative Compound Identifications from Mass Spectrometry Metabolomics Data: New Algorithms for Constructing Extracted Ion Chromatograms and Detecting Chromatographic Peaks","volume":"89","author":"Myers","year":"2017","journal-title":"Anal. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/s41592-020-0933-6","article-title":"Feature-Based Molecular Networking in the GNPS Analysis Environment","volume":"17","author":"Nothias","year":"2020","journal-title":"Nat. Methods"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1038\/s41587-020-0740-8","article-title":"Systematic Classification of Unknown Metabolites Using High-Resolution Fragmentation Mass Spectra","volume":"39","author":"Nothias","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e70780","DOI":"10.7554\/eLife.70780","article-title":"The LOTUS Initiative for Open Knowledge Management in Natural Products Research","volume":"11","author":"Rutz","year":"2022","journal-title":"eLife"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e1006089","DOI":"10.1371\/journal.pcbi.1006089","article-title":"Propagating Annotations of Molecular Networks Using in Silico Fragmentation","volume":"14","author":"Wang","year":"2018","journal-title":"PLoS Comput. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Ernst, M., Kang, K.B., Caraballo-Rodr\u00edguez, A.M., Nothias, L.-F., Wandy, J., Chen, C., Wang, M., Rogers, S., Medema, M.H., and Dorrestein, P.C. (2019). MolNetEnhancer: Enhanced Molecular Networks by Integrating Metabolome Mining and Annotation Tools. Metabolites, 9.","DOI":"10.1101\/654459"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s13321-016-0174-y","article-title":"ClassyFire: Automated Chemical Classification with a Comprehensive, Computable Taxonomy","volume":"8","author":"Eisner","year":"2016","journal-title":"J. Cheminformatics"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human Malaria Parasites in Continuous Culture","volume":"193","author":"Trager","year":"1976","journal-title":"Science"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e73","DOI":"10.1055\/a-1158-0569","article-title":"Heparin-Coated Liposomes Improve Antiplasmodial Activity and Reduce the Toxicity of Poupartone B","volume":"07","author":"Ledoux","year":"2020","journal-title":"Planta Med. Int. Open"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.jss.2007.04.021","article-title":"Characterization of HCT116 Human Colon Cancer Cells in an Orthotopic Model","volume":"147","author":"Rajput","year":"2008","journal-title":"J. Surg. Res."},{"key":"ref_66","unstructured":"Conn, P.M. (2013). Animal Models for the Study of Human Disease, Academic Press."},{"key":"ref_67","unstructured":"(2022, November 11). Dictonary of Natural Products Website. Available online: https:\/\/dnp.chemnetbase.com\/."},{"key":"ref_68","unstructured":"(2022, November 11). NPatlas Website. Available online: https:\/\/www.npatlas.org\/."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Le Goff, G., Lopes, P., Arcile, G., Vlachou, P., Van Elslande, E., Retailleau, P., Gallard, J.-F., Weis, M., Benayahu, Y., and Fokialakis, N. (2019). Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella Cavernosa. Mar. Drugs, 17.","DOI":"10.3390\/md17120678"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"8574084","DOI":"10.1155\/2020\/8574084","article-title":"Bioactivities and Future Perspectives of Chaetoglobosins","volume":"2020","author":"Chen","year":"2020","journal-title":"Evid. Based Complement Altern. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"3734","DOI":"10.1021\/jf500390h","article-title":"Chaetoglobosins from Chaetomium globosum, an Endophytic Fungus in Ginkgo biloba, and Their Phytotoxic and Cytotoxic Activities","volume":"62","author":"Li","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1039\/C4NP00167B","article-title":"The Marine Actinomycete Genus Salinispora: A Model Organism for Secondary Metabolite Discovery","volume":"32","author":"Jensen","year":"2015","journal-title":"Nat. Prod. Rep."},{"key":"ref_73","unstructured":"(2023, January 15). METACYC Website. Available online: https:\/\/metacyc.org\/."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"396","DOI":"10.7164\/antibiotics.55.396","article-title":"New Insights into Rifamycin B Biosynthesis: Isolation of Proansamycin B and 34a-Deoxy-Rifamycin W as Early Macrocyclic Intermediates Indicating Two Separated Biosynthetic Pathways","volume":"55","author":"Stratmann","year":"2002","journal-title":"J. Antibiot."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"253","DOI":"10.7164\/antibiotics.22.253","article-title":"Megalomicin, a New Macrolide Antibiotic Complex Produced by Micromonospora","volume":"22","author":"Weinstein","year":"1969","journal-title":"J. Antibiot."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3869","DOI":"10.1128\/AEM.03083-09","article-title":"TDP-l-Megosamine Biosynthesis Pathway Elucidation and Megalomicin A Production in Escherichia coli","volume":"76","author":"Useglio","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1046\/j.1365-2958.2000.02059.x","article-title":"Biosynthesis of the Anti-Parasitic Agent Megalomicin: Transformation of Erythromycin to Megalomicin in Saccharopolyspora erythraea","volume":"37","author":"Volchegursky","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1042\/bj1000169","article-title":"The Production of Tryptamine from Tryptophan by Bacillus cereus (KVT)","volume":"100","author":"Perley","year":"1966","journal-title":"Biochem. J."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Huang, S., Chen, H., Li, W., Zhu, X., Ding, W., and Li, C. (2016). Bioactive Chaetoglobosins from the Mangrove Endophytic Fungus Penicillium chrysogenum. Mar. Drugs, 14.","DOI":"10.3390\/md14100172"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1021\/acs.jnatprod.8b00103","article-title":"Staurosporine Derivatives Generated by Pathway Engineering in a Heterologous Host and Their Cytotoxic Selectivity","volume":"81","author":"Xiao","year":"2018","journal-title":"J. Nat. Prod."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"11273","DOI":"10.1021\/ja992482o","article-title":"Cyclomarins A\u2212C, New Antiinflammatory Cyclic Peptides Produced by a Marine Bacterium ( Streptomyces sp.)","volume":"121","author":"Renner","year":"1999","journal-title":"J. Am. Chem. Soc."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1021\/jo5024409","article-title":"Salinipostins A\u2013K, Long-Chain Bicyclic Phosphotriesters as a Potent and Selective Antimalarial Chemotype","volume":"80","author":"Schulze","year":"2015","journal-title":"J. Org. Chem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1028334","DOI":"10.3389\/fmolb.2022.1028334","article-title":"Inventa: A Computational Tool to Discover Structural Novelty in Natural Extracts Libraries","volume":"9","author":"Nothias","year":"2022","journal-title":"Front. Mol. Biosci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1021\/acs.jnatprod.6b00722","article-title":"Prioritizing Natural Product Diversity in a Collection of 146 Bacterial Strains Based on Growth and Extraction Protocols","volume":"80","author":"Larson","year":"2017","journal-title":"J. Nat. Prod."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"632230","DOI":"10.3389\/fbioe.2021.632230","article-title":"Recent Advances in Silent Gene Cluster Activation in Streptomyces","volume":"9","author":"Liu","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Liu, S.-W., Jadambaa, N., Nikandrova, A.A., Osterman, I.A., and Sun, C.-H. (2022). Exploring the Diversity and Antibacterial Potentiality of Cultivable Actinobacteria from the Soil of the Saxaul Forest in Southern Gobi Desert in Mongolia. Microorganisms, 10.","DOI":"10.3390\/microorganisms10050989"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"e0258934","DOI":"10.1371\/journal.pone.0258934","article-title":"Development of an Agar-Plug Cultivation System for Bioactivity Assays of Actinomycete Strain Collections","volume":"16","author":"Ortlieb","year":"2021","journal-title":"PLoS ONE"},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Crater, J.S., and Lievense, J.C. (2018). Scale-up of Industrial Microbial Processes. FEMS Microbiol. Lett., 365.","DOI":"10.1093\/femsle\/fny138"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Romano, S., Jackson, S.A., Patry, S., and Dobson, A.D.W. (2018). Extending the \u201cOne Strain Many Compounds\u201d (OSMAC) Principle to Marine Microorganisms. Mar. Drugs, 16.","DOI":"10.3390\/md16070244"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/3\/697\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:51:13Z","timestamp":1760122273000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/3\/697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,8]]},"references-count":89,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["microorganisms11030697"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11030697","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,8]]}}}