{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T12:30:58Z","timestamp":1774614658401,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,18]],"date-time":"2023-03-18T00:00:00Z","timestamp":1679097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bioeconomy International submission platform","award":["F\u00f6rderkennzeichen: 031B0814"],"award-info":[{"award-number":["F\u00f6rderkennzeichen: 031B0814"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>1,3-propanediol (1,3-PDO) is a valuable basic chemical, especially in the polymer industry to produce polytrimethylene terephthalate. Unfortunately, the production of 1,3-PDO mainly depends on petroleum products as precursors. Furthermore, the chemical routes have significant disadvantages, such as environmental issues. An alternative is the biobased fermentation of 1,3-PDO from cheap glycerol. Clostridium beijerinckii DSM 6423 was originally reported to produce 1,3-PDO. However, this could not be confirmed, and a genome analysis revealed the loss of an essential gene. Thus, 1,3-PDO production was genetically reinstalled. Genes for 1,3-PDO production from Clostridium pasteurianum DSM 525 and Clostridium beijerinckii DSM 15410 (formerly Clostridium diolis) were introduced into C. beijerinckii DSM 6423 to enable 1,3-PDO production from glycerol. 1,3-PDO production by recombinant C. beijerinckii strains were investigated under different growth conditions. 1,3-PDO production was only observed for C. beijerinckii [pMTL83251_Ppta-ack_1,3-PDO.diolis], which harbors the genes of C. beijerinckii DSM 15410. By buffering the growth medium, production could be increased by 74%. Furthermore, the effect of four different promoters was analyzed. The use of the constitutive thlA promoter from Clostridium acetobutylicum led to a 167% increase in 1,3-PDO production compared to the initial recombinant approach.<\/jats:p>","DOI":"10.3390\/microorganisms11030784","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T06:48:35Z","timestamp":1679294915000},"page":"784","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Heterologous 1,3-Propanediol Production Using Different Recombinant Clostridium beijerinckii DSM 6423 Strains"],"prefix":"10.3390","volume":"11","author":[{"given":"Teresa","family":"Schoch","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Biotechnologie, Universit\u00e4t Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4405-556X","authenticated-orcid":false,"given":"Tina","family":"Baur","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Biotechnologie, Universit\u00e4t Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna","family":"Kunz","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Biotechnologie, Universit\u00e4t Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"St\u00f6ferle","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Biotechnologie, Universit\u00e4t Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5586-221X","authenticated-orcid":false,"given":"Peter","family":"D\u00fcrre","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Biotechnologie, Universit\u00e4t Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,18]]},"reference":[{"key":"ref_1","first-page":"243","article-title":"Catalysts for glycerol hydrogenolysis to 1,3-propanediol: A review of chemical routes and market","volume":"381","author":"Hewer","year":"2020","journal-title":"Catal. 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