{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T18:10:02Z","timestamp":1769278202165,"version":"3.49.0"},"reference-count":71,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004242","name":"Princess Nourah bint Abdulrahman University","doi-asserted-by":"publisher","award":["PNURSP2023R214"],"award-info":[{"award-number":["PNURSP2023R214"]}],"id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Daphnia magna and freshwater snails are used as delicate bioindicators of contaminated aquatic habitats. Due to their distinctive characteristics, selenium oxide nanoparticles (SeONPs) have received interest regarding their possible implications on aquatic environments. The current study attempted to investigate the probable mechanisms of fungal-mediated selenium nanoparticles\u2019 ecotoxicological effects on freshwater Biomphalaria alexandrina snails and Daphnia magna. SeONPs revealed a toxicological impact on D. magna, with a half-lethal concentration (LC50) of 1.62 mg\/L after 24 h and 1.08 mg\/L after 48 h. Survival, fecundity, and reproductive rate were decreased in B. alexandrina snails exposed to SeONPs. Furthermore, the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were markedly elevated, while albumin and total protein levels decreased. Histopathological damage in the hermaphrodite and digestive glands was detected by light, electron microscopy, and immunohistochemistry studies. The molecular docking study revealed interactions of selenium oxide with the ALT and AST. In conclusion, B. alexandrina snails and D. magna could be employed as bioindicators of selenium nanomaterial pollution in aquatic ecosystems. This study emphasizes the possible ecological effects of releasing SeONPs into aquatic habitats, which could serve as motivation for regulatory organizations to monitor and control the use and disposal of SeONPs in industry.<\/jats:p>","DOI":"10.3390\/microorganisms11030811","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T07:46:43Z","timestamp":1679471203000},"page":"811","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Histopathological, Immunohistochemical, Biochemical, and In Silico Molecular Docking Study of Fungal-Mediated Selenium Oxide Nanoparticles on Biomphalaria alexandrina (Ehrenberg, 1831) Snails"],"prefix":"10.3390","volume":"11","author":[{"given":"Amina M.","family":"Ibrahim","sequence":"first","affiliation":[{"name":"Medical Malacology Department, Theodor Bilharz Research Institute, Giza 12411, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahassen","family":"Ghazy","sequence":"additional","affiliation":[{"name":"Water Pollution Research Department, National Research Centre, El Tahrir Street, Dokki, Giza 12622, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2962-6755","authenticated-orcid":false,"given":"Heba","family":"El-Sayed","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan 11795, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rehab M.","family":"Abd El-Hameed","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan 11795, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rehab G.","family":"Khalil","sequence":"additional","affiliation":[{"name":"Immunology Division, Faculty of Science, Beni-Suef University, Beni-Suef 62521, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9355-3952","authenticated-orcid":false,"given":"Shereen M.","family":"Korany","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7712-0313","authenticated-orcid":false,"given":"Abeer S.","family":"Aloufi","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olfat A.","family":"Hammam","sequence":"additional","affiliation":[{"name":"Pathology Departments, Theodor Bilharz Research Institute, Giza 12411, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9005-7032","authenticated-orcid":false,"given":"Mostafa Y.","family":"Morad","sequence":"additional","affiliation":[{"name":"Zoology and Entomology Department, Faculty of Science, Helwan University, Helwan 11795, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"133511","DOI":"10.1016\/j.chemosphere.2021.133511","article-title":"Assessment of Toxicity in the Freshwater Tadpole Polypedates Maculatus Exposed to Silver and Zinc Oxide Nanoparticles: A Multi-Biomarker Approach","volume":"293","author":"Murthy","year":"2022","journal-title":"Chemosphere"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s00604-001-0899-8","article-title":"Determination of Selenium Species in Environmental Samples","volume":"140","year":"2002","journal-title":"Microchim. 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