{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T17:49:09Z","timestamp":1776361749773,"version":"3.51.2"},"reference-count":43,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This study investigated the effects of dietary commercial feed (n = 50,025 in triplicate, named group PF for soil dike pond, sampling n = 7; n = 15,000 in triplicate, WF for water tank, n = 8), iced fish (n = 50,025 in triplicate, PI, n = 7), and a combination of both (n = 50,025 in triplicate, PFI, n = 8) on different metabolic parameters of the largemouth bass, Micropterus salmoides (0.67 \u00b1 0.09 g, culture period from June 2017 to July 2018). Throughout the experimental period, different areas of water (including input water of the front, middle of the pond, and from the drain off at the back) and their mixed samples were simultaneously analyzed to find the source of the main infectious bacteria. Various feeding strategies may differentially affect body composition and shape the gut microbiota, but the mode of action has not been determined. Results showed that no significant differences were found in the growth performance except for the product yield using a different culture mode (PFI vs. WF). For muscle composition, the higher \u2211SFA, \u2211MUFA, \u2211n-6PUFA, and 18:3n-3\/18:2n-6 levels were detected in largemouth bass fed with iced fish, while enrichment in \u2211n-3PUFA and \u2211HUFA was detected in largemouth bass fed with commercial feed. For the gut microbiota, Fusobacteria, Proteobacteria, and Firmicutes were the most dominant phyla among all the gut samples. The abundance of Firmicutes and Tenericutes significantly decreased and later increased with iced fish feeding. The relative abundance of species from the Clostridia, Mollicutes, Mycoplasmatales, and families (Clostridiaceae and Mycoplasmataceae) significantly increased in the feed plus iced fish (PFI) group relative to that in the iced fish (PI) group. Pathways of carbohydrate metabolism and the digestive system were enriched in the commercial feed group, whereas infectious bacterial disease resistance-related pathways were enriched in the iced fish group, corresponding to the higher rate of death, fatty liver disease, and frequency and duration of cyanobacteria outbreaks. Feeding with iced fish resulted in more activities in the digestive system and energy metabolism, more efficient fatty acid metabolism, had higher \u2211MUFA, and simultaneously had the potential for protection against infectious bacteria from the environment through a change in intestinal microbiota in the pond of largemouth bass culturing. Finally, the difference in feed related to the digestive system may contribute to the significant microbiota branch in the fish gut, and the input and outflow of water affects the intestinal flora in the surrounding water and in the gut, which in turn affects growth and disease resistance.<\/jats:p>","DOI":"10.3390\/microorganisms11051356","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T05:49:32Z","timestamp":1684734572000},"page":"1356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Effects of Different Feeding Regimes on Body Composition, Gut Microbial Population, and Susceptibility to Pathogenic Infection in Largemouth Bass"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5958-6008","authenticated-orcid":false,"given":"Yao","family":"Zheng","sequence":"first","affiliation":[{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haojun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanjie","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gangchun","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1007\/s10695-021-00998-7","article-title":"Stocking density alters growth performance, serum biochemistry, digestive enzymes, immune response, and muscle quality of largemouth bass (Micropterus salmoides) in in-pond raceway system","volume":"47","author":"Yuan","year":"2021","journal-title":"Fish Physiol. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"696588","DOI":"10.3389\/fmicb.2021.696588","article-title":"A high starch diet alters the composition of the intestinal microbiota of largemouth bass Micropterus salmoides, which may be associated with the development of enteritis","volume":"12","author":"Huang","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1111\/jam.14174","article-title":"The composition and structure of the intestinal microflora of Anguilla marmorata at different growth rates: A deep sequencing study","volume":"126","author":"Lin","year":"2019","journal-title":"J. Appl. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1007\/s10695-019-00636-3","article-title":"Linseed oil can decrease liver fat deposition and improve antioxidant ability of juvenile largemouth bass, Micropterus salmoides","volume":"45","author":"Shi","year":"2019","journal-title":"Fish Physiol. Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"137579","DOI":"10.1155\/2013\/137579","article-title":"Lipotoxicity: Effects of dietary saturated and transfatty acids","volume":"2013","author":"Estadella","year":"2013","journal-title":"Mediat. Inflamm."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"106","DOI":"10.3389\/fnut.2018.00106","article-title":"Resveratrol modulates desaturase expression and fatty acid composition of cultured hepatocytes","volume":"5","author":"Pallauf","year":"2018","journal-title":"Front. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.envpol.2018.05.027","article-title":"Tracking pollutants in dietary fish oil: From ocean to table","volume":"240","author":"Sun","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cbpb.2017.05.002","article-title":"Effects of nutritional history on stress response in gibel carp (Carassius auratus gibelio) and largemouth bass (Micropterus salmoides)","volume":"210","author":"Jiang","year":"2017","journal-title":"Comp. Biochem. Physiol. B"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40168-018-0400-0","article-title":"Effects of predation stress and food ration on perch gut microbiota","volume":"6","author":"Zha","year":"2018","journal-title":"Microbiome"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.fsi.2019.04.042","article-title":"Varieties of immunity activities and gut contents in tilapia with seasonal changes","volume":"90","author":"Zheng","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1128\/AEM.02747-12","article-title":"Impact of long-term diesel contamination on soil microbial community structure","volume":"79","author":"Sutton","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1111\/1755-0998.12586","article-title":"Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio","volume":"17","author":"Minamoto","year":"2017","journal-title":"Mol. Ecol. Resour."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1007\/s10695-011-9491-2","article-title":"Effects of a diet lacking HUFA on lipid and fatty acid content of intestine and gills of male gilthead seabream (Sparus aurata L.) broodstock at different stages of the reproductive cycle","volume":"37","author":"Almansa","year":"2011","journal-title":"Fish Physiol. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Li, Y., Pang, Y., Xiang, X., Du, J., Mai, K., and Ai, Q. (2019). Molecular cloning, characterization, and nutritional regulation of elovl6 in large yellow croaker (Larimichthys crocea). Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20071801"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Wei, J., Guo, X., Liu, H., Chen, Y., and Wang, W. (2018). The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition. BMC Microbiol., 18.","DOI":"10.1186\/s12866-018-1246-0"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Li, X., Yan, Q., Ring\u00f8, E., Wu, X., He, Y., and Yang, D. (2016). The influence of weight and gender on intestinal bacterial community of wild largemouth bronze gudgeon (Coreius guichenoti, 1874). BMC Microbiol., 16.","DOI":"10.1186\/s12866-016-0809-1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e6963","DOI":"10.7717\/peerj.6963","article-title":"Dietary supplementation with probiotics regulates gut microbiota structure and function in Nile tilapia exposed to aluminum","volume":"7","author":"Yu","year":"2019","journal-title":"PeerJ"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"30606","DOI":"10.1038\/srep30606","article-title":"Alterations of the gut microbiome of largemouth bronze gudgeon (Coreius guichenoti) suffering from furunculosis","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.4315\/0362-028X.JFP-17-498","article-title":"Characterization of the microbiota in air- or vacuum-packed crisp grass carp (Ctenopharyngodon idella C. et V.) fillets by 16S rRNA PCR-denaturing gradient gel electrophoresis and high-throughput sequencing","volume":"81","author":"Pan","year":"2018","journal-title":"J. Food Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.wasman.2020.08.010","article-title":"Intermittent aeration of landfill simulation bioreactors: Effects on emissions and microbial community","volume":"117","author":"Campanaro","year":"2020","journal-title":"Waste Manag."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1857515","DOI":"10.1080\/19490976.2020.1857515","article-title":"Pomegranate peel extract ameliorates the severity of experimental autoimmune encephalomyelitis via modulation of gut microbiota","volume":"12","author":"Lu","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"198581","DOI":"10.1016\/j.virusres.2021.198581","article-title":"Screening of a Plesiomonas shigelloides phage and study of the activity of its lysis system","volume":"306","author":"Zhang","year":"2021","journal-title":"Virus Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1007\/s10695-021-01004-w","article-title":"Dietary sodium butyrate supplementation attenuates intestinal inflammatory response and improves gut microbiota composition in largemouth bass (Micropterus salmoides) fed with a high soybean meal diet","volume":"47","author":"Chen","year":"2021","journal-title":"Fish Physiol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1089\/zeb.2020.1955","article-title":"Pseudocapillaria tomentosa, Mycoplasma spp.; and intestinal lesions in experimentally infected zebrafish Danio rerio","volume":"18","author":"Kent","year":"2021","journal-title":"Zebrafish"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wu, Z., Zhang, Q., Lin, Y., Hao, J., Wang, S., Zhang, J., and Li, A. (2021). Taxonomic and functional characteristics of the gill and gastrointestinal microbiota and its correlation with intestinal metabolites in new GIFT strain of farmed adult nile tilapia (Oreochromis niloticus). Microorganisms, 9.","DOI":"10.3390\/microorganisms9030617"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1007\/s10126-019-09939-1","article-title":"Atlantic salmon (Salmo salar L., 1758) gut microbiota profile correlates with flesh pigmentation: Cause or effect?","volume":"22","author":"Nguyen","year":"2020","journal-title":"Mar. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/19490976.2021.1900996","article-title":"Intestinal Cetobacterium and acetate modify glucose homeostasis via parasympathetic activation in zebrafish","volume":"13","author":"Wang","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nrgastro.2012.165","article-title":"Gut microbiota: Married to our gut microbiota","volume":"9","author":"Ray","year":"2012","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e5906","DOI":"10.7717\/peerj.5906","article-title":"Microbiome differences between river-dwelling and cave-adapted populations of the fish Astyanax mexicanus (De Filippi, 1853)","volume":"6","author":"Pajares","year":"2018","journal-title":"PeerJ"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Xu, G., Xing, W., Li, T., Xue, M., Ma, Z., Jiang, N., and Luo, L. (2019). Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1673-6"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e6000","DOI":"10.7717\/peerj.6000","article-title":"Intestinal microbiome and its potential functions in bighead carp (Aristichthys nobilis) under different feeding strategies","volume":"6","author":"Li","year":"2018","journal-title":"PeerJ"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1111\/jam.12626","article-title":"Do the intestinal microbiotas differ between paddlefish (Polyodon spathala) and bighead carp (Aristichthys nobilis) reared in the same pond?","volume":"117","author":"Li","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.fsi.2017.08.035","article-title":"Effects of Astragalus polysaccharides (APS) and chitooligosaccharides (COS) on growth, immune response and disease resistance of juvenile largemouth bass, Micropterus salmoides","volume":"70","author":"Lin","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Moln\u00e1r, T., Csuv\u00e1r, A., Benedek, I., Moln\u00e1r, M., and Kabai, P. (2018). Domestication affects exploratory behaviour of pikeperch (Sander lucioperca L.) during the transition to pelleted food. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196118"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s13568-019-0745-1","article-title":"Coupled changes of bacterial community and function in the gut of mud crab (Scylla paramamosain) in response to Baimang disease","volume":"9","author":"Deng","year":"2019","journal-title":"AMB Express"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1007\/s00248-006-9037-9","article-title":"16S rDNA-based analysis of dominant bacterial populations associated with early life stages of coho salmon (Oncorhynchus kisutch)","volume":"51","author":"Romero","year":"2006","journal-title":"Microb. Ecol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/1462-2920.12614","article-title":"Identification of active denitrifiers in full-scale nutrient removal wastewater treatment systems","volume":"18","author":"McIlroy","year":"2016","journal-title":"Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.2217\/fmb.15.66","article-title":"Probiotics Clostridium butyricum and Bacillus subtilis ameliorate intestinal tumorigenesis","volume":"10","author":"Chen","year":"2015","journal-title":"Future Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s00284-012-0090-y","article-title":"\u201cMycoplasmal antigen modulation\u201d, a novel surface variation suggested for a lipoprotein specifically localized on Mycoplasma mobile","volume":"64","author":"Wu","year":"2012","journal-title":"Curr. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.fsi.2014.12.008","article-title":"Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus","volume":"43","author":"Iwashita","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.fsi.2020.06.061","article-title":"Bacillus subtilis H2 modulates immune response, fat metabolism and bacterial flora in the gut of grass carp (Ctenopharyngodon idellus)","volume":"106","author":"Shi","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e00716","DOI":"10.1002\/mbo3.716","article-title":"Microbial effects of livestock manure fertilization on freshwater aquaculture ponds rearing tilapia (Oreochromis shiranus) and North African catfish (Clarias gariepinus)","volume":"7","author":"Minich","year":"2018","journal-title":"Microbiologyopen"},{"key":"ref_43","first-page":"100924","article-title":"Alterations of amino acid metabolism and intestinal microbiota in Chinese mitten crab (Eriocheir sinensis) fed on formulated diet and iced trash fish","volume":"40","author":"Feng","year":"2021","journal-title":"Comp. Biochem. Physiol. D"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/5\/1356\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:39:51Z","timestamp":1760125191000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/5\/1356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,22]]},"references-count":43,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["microorganisms11051356"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11051356","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,22]]}}}