{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:05:12Z","timestamp":1774958712579,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Executive Agency for Higher Education, Research, Development and Innovation Funding (UEFISCDI), Romania","award":["PD 100\/2020"],"award-info":[{"award-number":["PD 100\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The aim of the current study was to investigate the prevalence of Clostridium perfringens (C. perfringens) recovered from animal faeces, as well as to determine the antimicrobial susceptibility of such isolates. A total of 14 (14\/100; 14%) C. perfringens isolates were isolated from the 100 analysed samples (twelve recovered from faecal samples collected from pigs and two from veal calves\u2019 faecal samples). The preponderant genotype was type A, with all isolates being cpa-positive. The most potent antimicrobial agents against C. perfringens proved to be vancomycin, rifampicin and lincomycin. A strong resistance to tetracycline (71.4%), penicillin (64.2%), erythromycin (42.8%) and enrofloxacin (35.7%) was also observed. To the best of our knowledge, this is the first analysis regarding the prevalence, characterization and antimicrobial susceptibility of C. perfringens in food-producing animals in Romania, adding further evidence for the probable role of animals as a source of resistant C. perfringens strains.<\/jats:p>","DOI":"10.3390\/microorganisms11061373","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T01:36:28Z","timestamp":1684978588000},"page":"1373","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Prevalence and Characterisation of Clostridium perfringens Isolates in Food-Producing Animals in Romania"],"prefix":"10.3390","volume":"11","author":[{"given":"Corina","family":"Beres","sequence":"first","affiliation":[{"name":"Department of Animal Breeding and Food Science, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Manastur Street No. 3\/5, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1577-4861","authenticated-orcid":false,"given":"Liora","family":"Colobatiu","sequence":"additional","affiliation":[{"name":"Department of Medical Devices, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes Street No. 8, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Tabaran","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding and Food Science, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Manastur Street No. 3\/5, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romolica","family":"Mihaiu","sequence":"additional","affiliation":[{"name":"Department of Management, Faculty of Economic Sciences and Business Administration, Babes Bolyai University, Mihail Kogalniceanu Street No.1, 400084 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Mihaiu","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding and Food Science, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Manastur Street No. 3\/5, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.resmic.2014.09.004","article-title":"Clostridium perfringens type A\u2013E toxinplasmids","volume":"166","author":"Freedman","year":"2015","journal-title":"Res. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.smallrumres.2019.02.011","article-title":"Distribution and antimicrobial resistance patterns of Clostridium perfringens isolated from vaccinated and unvaccinated goats","volume":"173","author":"Khan","year":"2019","journal-title":"Small Rumin. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102235","DOI":"10.1016\/j.anaerobe.2020.102235","article-title":"Prevalence, toxin-typing, and antimicrobial susceptibility of Clostridium perfringens from retail meats in Seoul, Korea","volume":"64","author":"Jang","year":"2020","journal-title":"Anaerobe"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.anaerobe.2014.11.004","article-title":"Detection and molecular typing of Clostridium perfringens isolates from beef, chicken and turkey meats","volume":"32","author":"Aras","year":"2015","journal-title":"Anaerobe"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"102499","DOI":"10.1016\/j.anaerobe.2021.102499","article-title":"Isolation, toxinotyping and antimicrobial susceptibility testing of Clostridium perfringens isolated from Pakistan poultry","volume":"73","author":"Haider","year":"2022","journal-title":"Anaerobe"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.anaerobe.2018.04.011","article-title":"Expansion of the Clostridium perfringens toxin-based typing scheme","volume":"53","author":"Rood","year":"2018","journal-title":"Anaerobe"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7792","DOI":"10.1038\/s41598-019-44281-5","article-title":"Prevalence and characterization of Clostridium perfringens toxinotypes among patients with antibiotic-associated diarrhea in Iran","volume":"9","author":"Azimirad","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S0966-842X(98)01430-9","article-title":"Clostridium perfringens: Toxinotype and genotype","volume":"7","author":"Petit","year":"1999","journal-title":"Trends Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.mimet.2016.06.007","article-title":"Development and application of a multiplex PCR assay for detection of the Clostridium perfringens enterotoxin-encoding genes cpe and becAB","volume":"127","author":"Yonogi","year":"2016","journal-title":"J. Microbiol. Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"108932","DOI":"10.1016\/j.vetmic.2020.108932","article-title":"Prevalence and antimicrobial susceptibility of Clostridium perfringens in chickens and pigs from Beijing and Shanxi, China","volume":"252","author":"Li","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.vetmic.2004.01.009","article-title":"Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of Clostridium perfringens from poultry, and distribution of tetracycline resistance genes","volume":"99","author":"Johansson","year":"2004","journal-title":"Vet. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.anaerobe.2005.10.004","article-title":"Anaerobes: Antibiotic resistance, clinical significance, and the role of susceptibility testing","volume":"12","author":"Hecht","year":"2006","journal-title":"Anaerobe"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s13099-015-0063-3","article-title":"First description of plasmid-mediated quinolone resistance determinants and \u03b2-lactamase encoding genes in non-typhoidal Salmonella isolated from humans, one companion animal and food in Romania","volume":"7","author":"Colobatiu","year":"2015","journal-title":"Gut. Pathog."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41426-018-0144-8","article-title":"An update on the human and animal enteric pathogen Clostridium perfringens","volume":"7","author":"Kiu","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1472-765X.2008.02379.x","article-title":"Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR","volume":"47","author":"Erol","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"702","DOI":"10.2460\/ajvr.1997.58.07.702","article-title":"Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens","volume":"58","author":"Meer","year":"1997","journal-title":"Am. J. Vet. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.1128\/IAI.01759-14","article-title":"BEC, a Novel Enterotoxin of Clostridium perfringens Found in Human Clinical Isolates from Acute Gastroenteritis Outbreaks","volume":"82","author":"Yonogi","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_18","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2018). Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, CLSI. [9th ed.]."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"102298","DOI":"10.1016\/j.anaerobe.2020.102298","article-title":"Toxinotyping and molecular characterization of antimicrobial resistance in Clostridium perfringens isolated from different sources of livestock and poultry","volume":"67","author":"Anju","year":"2021","journal-title":"Anaerobe"},{"key":"ref_20","unstructured":"Willis, A.T. (1977). Anaerobic Bacteriology: Clinical and Laboratory Practice, Butterworths."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101460","DOI":"10.1016\/j.cimid.2020.101460","article-title":"Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from dromedary camels, pastures and herders","volume":"70","author":"Fayez","year":"2020","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_22","unstructured":"Toldra, F. (2017). Lawrie\u2019s Meat Science, Woodhead Publishing."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2016.03.017","article-title":"Antimicrobial susceptibility of Clostridium difficile isolated from food animals on farms","volume":"227","author":"Thitaram","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.foodres.2018.07.040","article-title":"Distribution and tracking of Clostridium difficile and Clostridium perfringens in a free-range pig abattoir and processing plant","volume":"113","author":"Blanco","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.anaerobe.2017.05.009","article-title":"Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment","volume":"47","author":"Yadav","year":"2017","journal-title":"Anaerobe"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.anaerobe.2018.08.007","article-title":"Prevalence and characterization of Clostridium perfringens in broiler chickens and retail chicken meat in central China","volume":"54","author":"Zhang","year":"2018","journal-title":"Anaerobe"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2685","DOI":"10.1128\/AEM.70.5.2685-2691.2004","article-title":"Detection of Enterotoxigenic Clostridium perfringens Type A Isolates in American Retail Foods","volume":"70","author":"Wen","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.anaerobe.2018.06.011","article-title":"Molecular genotyping, biofilm formation and antibiotic resistance of enterotoxigenic Clostridium perfringens isolated from meat supplied to school cafeterias in South Korea","volume":"52","author":"Hu","year":"2018","journal-title":"Anaerobe"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.3389\/fvets.2022.1022215","article-title":"Antimicrobial susceptibility and multilocus sequence typing of Clostridium perfringens isolated from yaks in Qinghai-Tibet plateau, China","volume":"9","author":"Wu","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.anaerobe.2015.12.012","article-title":"Multidrug resistance in Clostridium perfringens isolated from diarrheal neonatal piglets in Thailand","volume":"38","author":"Ngamwongsatit","year":"2016","journal-title":"Anaerobe"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"931","DOI":"10.20506\/rst.31.3.2169","article-title":"Prevalence of Clostridium perfringens type A isolates in commercial broiler chickens and parent broiler breeder hens in Egypt","volume":"31","author":"Osman","year":"2012","journal-title":"Rev. Sci. Tech."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1007\/s11259-009-9306-4","article-title":"Antimicrobial resistance in Clostridium perfringens isolates from broilers in Belgium","volume":"33","author":"Gholamiandehkordi","year":"2009","journal-title":"Vet. Res. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chan, G., Farzan, A., Soltes, G., Nicholson, V.M., Pei, Y., Friendship, R., and Prescott, J.F. (2012). The epidemiology of Clostridium perfringens type A on Ontario swine farms, with special reference to cpb2-positive isolates. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-156"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.anaerobe.2018.01.001","article-title":"Diversity of toxin-genotypes among Clostridium perfringens isolated from healthy and diarrheic neonatal cattle and buffalo calves","volume":"49","author":"Athira","year":"2018","journal-title":"Anaerobe"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"24","DOI":"10.2174\/1875414701003020024","article-title":"Clostridium Perfringens Toxins Involved in Mammalian Veterinary Diseases","volume":"3","author":"Uzal","year":"2010","journal-title":"Open Toxinol. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1177\/104063870501700602","article-title":"Clostridial Enteric Infections in Pigs","volume":"17","author":"Songer","year":"2005","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1128\/AEM.02459-09","article-title":"Prevalence and Diversity of Toxigenic Clostridium perfringens and Clostridium difficile among Swine Herds in the Midwest","volume":"76","author":"Baker","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.3382\/ps.2009-00131","article-title":"Clostridium perfringens occurrence and ribotypes in healthy broilers reared in different European countries","volume":"88","author":"Borilova","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101414","DOI":"10.1016\/j.psj.2021.101414","article-title":"Research Note: Prevalence and molecular characteristics of Clostridium perfringens in \u201cno antibiotics ever\u201d broiler farms","volume":"100","author":"Fancher","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5366","DOI":"10.1128\/AEM.00783-08","article-title":"Prevalence and Characterization of Enterotoxin Gene-Carrying Clostridium perfringens Isolates from Retail Meat Products in Japan","volume":"74","author":"Miki","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"290","DOI":"10.9735\/0975-5276.4.7.290-294","article-title":"Toxinotyping and antimicrobial susceptibility of Clostridium perfringens isolated from broiler chickens with necrotic enteritis","volume":"4","author":"Llanco","year":"2012","journal-title":"Int. J. Microbiol. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s10068-017-0075-5","article-title":"Prevalence and toxin type of Clostridium perfringens in beef from four different types of meat markets in Seoul, Korea","volume":"26","author":"Jeong","year":"2017","journal-title":"Food Sci. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.anaerobe.2014.05.012","article-title":"Clostridium perfringens and Clostridium difficile in cooked beef sold in C\u00f4te d\u2019Ivoire and their antimicrobial susceptibility","volume":"28","author":"Kouassi","year":"2014","journal-title":"Anaerobe"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5134764","DOI":"10.1155\/2020\/5134764","article-title":"Scientific Achievements in the Study of the Occurrence and Antimicrobial Susceptibility Profile of Major Foodborne Pathogenic Bacteria in Foods and Food Processing Environments in Romania: Review of the Last Decade","volume":"2020","author":"Imre","year":"2020","journal-title":"BioMed Res. Int."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/6\/1373\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:40:48Z","timestamp":1760125248000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/6\/1373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,24]]},"references-count":44,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["microorganisms11061373"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11061373","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,24]]}}}