{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:49:12Z","timestamp":1789051752741,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Committee of the University of West Attica (UNIWA)","award":["8068\/07-04-2020"],"award-info":[{"award-number":["8068\/07-04-2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Antibiotic-resistant bacteria (ARB) are present in wastewaters as their elimination during treatment in wastewater treatment plants (WWTPs) is often impossible. Water plays an important role in the spread of these microorganisms among humans, animals and the environment. This study aimed to assess the antimicrobial resistance patterns, resistance genes and molecular genotypes by means of phylogenetic groups of E. coli isolates in aquatic habitats, including sewage and receiving water bodies, as well as clinical settings in the Boeotia regional district of Greece. The highest resistance rates among both environmental and clinical isolates were observed to be for penicillins, ampicillin and piperacillin. Resistance patterns related to extended spectrum \u03b2-lactamases (ESBL) production and ESBL genes were also detected in both environmental and clinical isolates. Phylogenetic group B2 was predominant in clinical settings and the second most frequent among wastewaters, whereas group A was dominant in all environmental isolates. In conclusion, the studied river water and wastewaters may serve as reservoirs of resistant E. coli isolates that pose potential threats to both human and animal health.<\/jats:p>","DOI":"10.3390\/microorganisms11061399","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T01:52:43Z","timestamp":1685065963000},"page":"1399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Characterization and Prevalence of Antimicrobial-Resistant Escherichia coli Isolates Derived from Clinical Specimens and Environmental Habitats"],"prefix":"10.3390","volume":"11","author":[{"given":"Chrysoula","family":"Dioli","sequence":"first","affiliation":[{"name":"Molecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Pappa","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eirini","family":"Siatravani","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, Greece"},{"name":"Laboratory of Bacteriology, Hellenic Pasteur Institute, 127 Vasilissis Sofias Avenue, 11521 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spyridoula","family":"Bratakou","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apostolos","family":"Tatsiopoulos","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, General Hospital of Livadeia, Agiou Vlasiou, 32100 Livadia, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiota","family":"Giakkoupi","sequence":"additional","affiliation":[{"name":"Department of Public Health Policy, School of Public Health, University of West Attica, 196 Alexandras Avenue, 11521 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3905-0315","authenticated-orcid":false,"given":"Vivi","family":"Miriagou","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Hellenic Pasteur Institute, 127 Vasilissis Sofias Avenue, 11521 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5639-0528","authenticated-orcid":false,"given":"Apostolos","family":"Beloukas","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Immunology Laboratory, Department of Biomedical Sciences, University of West Attica, 28 Ag. Spyridonos Str., 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1080\/15287390600975137","article-title":"Potential ecological and human health impacts of antibiotics and antibiotic-resistant bacteria from wastewater treatment plants","volume":"10","author":"Kim","year":"2007","journal-title":"J. Toxicol. Environ. Health Part B Crit. Rev."},{"key":"ref_2","unstructured":"World Health Organization (2014). 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