{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T07:56:47Z","timestamp":1786780607261,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T00:00:00Z","timestamp":1685145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01ES028788"],"award-info":[{"award-number":["1R01ES028788"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Consumed food travels through the gastrointestinal tract to reach the small intestine, where it interacts with the microbiota, forming a complex relationship with the dietary components. Here we present a complex in vitro cell culture model of the small intestine that includes human cells, digestion, a simulated meal, and a microbiota represented by a bacterial community consisting of E. coli, L. rhamnosus, S. salivarius, B. bifidum, and E. faecalis. This model was used to determine the effects of food-grade titanium dioxide nanoparticles (TiO2 NPs), a common food additive, on epithelial permeability, intestinal alkaline phosphatase activity, and nutrient transport across the epithelium. Physiologically relevant concentrations of TiO2 had no effect on intestinal permeability but caused an increase in triglyceride transport as part of the food model, which was reversed in the presence of bacteria. Individual bacterial species had no effect on glucose transport, but the bacterial community increased glucose transport, suggesting a change in bacterial behavior when in a community. Bacterial entrapment within the mucus layer was reduced with TiO2 exposure, which may be due to decreased mucus layer thickness. The combination of human cells, a synthetic meal, and a bacterial mock community provides an opportunity to understand the implications of nutritional changes on small intestinal function, including the microbiota.<\/jats:p>","DOI":"10.3390\/microorganisms11061419","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T16:10:53Z","timestamp":1685203853000},"page":"1419","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["An In Vitro Small Intestine Model Incorporating a Food Matrix and Bacterial Mock Community for Intestinal Function Testing"],"prefix":"10.3390","volume":"11","author":[{"given":"Mridu","family":"Malik","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USA"},{"name":"Binghamton Biofilm Research Center, Binghamton University, Binghamton, NY 13902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob V.","family":"Tanzman","sequence":"additional","affiliation":[{"name":"Binghamton Biofilm Research Center, Binghamton University, Binghamton, NY 13902, USA"},{"name":"Department of Biological Sciences, Binghamton University, Binghamton, NY 13902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8278-8262","authenticated-orcid":false,"given":"Sanat Kumar","family":"Dash","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USA"},{"name":"Binghamton Biofilm Research Center, Binghamton University, Binghamton, NY 13902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2873-7074","authenticated-orcid":false,"given":"Cl\u00e1udia N. H.","family":"Marques","sequence":"additional","affiliation":[{"name":"Binghamton Biofilm Research Center, Binghamton University, Binghamton, NY 13902, USA"},{"name":"Department of Biological Sciences, Binghamton University, Binghamton, NY 13902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7286-8920","authenticated-orcid":false,"given":"Gretchen J.","family":"Mahler","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USA"},{"name":"Binghamton Biofilm Research Center, Binghamton University, Binghamton, NY 13902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1515\/BC.2009.052","article-title":"Management of the Human Mucosal Defensive Barrier: Evidence for Glycan Legislation","volume":"390","author":"Patsos","year":"2009","journal-title":"Biol. 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