{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T16:09:46Z","timestamp":1782749386384,"version":"3.54.5"},"reference-count":77,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T00:00:00Z","timestamp":1685318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"framework of the Order of the Government of the Russian Federation dated 22 December 2017 No. 2904-r (2018\u20132020)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: Q fever is a zoonotic infectious disease characterized by fever, malaise, chills, significant weakness, and muscle pain. In some cases, the disease can become chronic and affect the inner membranes of the heart, such as the valves, leading to endocarditis and a high risk of death. Coxiella burnetii (C. burnetii) is the primary causative agent of Q fever in humans. This study aims to monitor the presence of C. burnetii in ticks collected from small mammals and cattle in the Republic of Guinea (RG). Methods: Rodents were trapped in the Kindia region of RG during 2019\u20132020, and ticks were collected from cattle in six regions of RG. Total DNA was extracted using a commercial kit (RIBO-prep, InterLabService, Russia) following the manufacturer\u2019s instructions. Real-time PCR amplification was conducted using the kit (AmpliSens Coxiella burnetii-FL, InterLabService, Russia) to detect C. burnetii DNA. Results and Conclusions: Bacterial DNA was detected in 11 out of 750 (1.4%) small mammals and 695 out of 9620 (7.2%) tick samples. The high number of infected ticks (7.2%) suggests that they are the main transmitters of C. burnetii in RG. The DNA was detected in the liver and spleen of a Guinea multimammate mouse, Mastomys erythroleucus. These findings demonstrate that C. burnetii is zoonotic in RG, and measures should be taken to monitor the bacteria\u2019s dynamics and tick prevalence in the rodent population.<\/jats:p>","DOI":"10.3390\/microorganisms11061433","type":"journal-article","created":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T07:49:23Z","timestamp":1685346563000},"page":"1433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Epidemiology of Zoonotic Coxiella burnetii in The Republic of Guinea"],"prefix":"10.3390","volume":"11","author":[{"given":"Olesia V.","family":"Ohlopkova","sequence":"first","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey A.","family":"Yakovlev","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u00abMicrobe\u00bb of Rospotrebnadzor, Saratov 410005, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4328-8190","authenticated-orcid":false,"given":"Kabwe","family":"Emmanuel","sequence":"additional","affiliation":[{"name":"OpenLab \u201cGene and Cell Technologies\u201d, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey A.","family":"Kabanov","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A.","family":"Odnoshevsky","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail Yu.","family":"Kartashov","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey D.","family":"Moshkin","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor V.","family":"Tuchkov","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u00abMicrobe\u00bb of Rospotrebnadzor, Saratov 410005, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita Yu.","family":"Nosov","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u00abMicrobe\u00bb of Rospotrebnadzor, Saratov 410005, Russia"},{"name":"State Research Center of Dermatovenerology and Cosmetology of Russian Ministry of Health, Moscow 107076, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey A.","family":"Kritsky","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u00abMicrobe\u00bb of Rospotrebnadzor, Saratov 410005, Russia"},{"name":"Limited Liability Company, \u00abBiotech Campus\u00bb, Moscow 117437, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milana A.","family":"Agalakova","sequence":"additional","affiliation":[{"name":"Faculty of Preventive Medicine, Ural State Medical University, Yekaterinburg 620014, Russia"},{"name":"Limited Liability Company, \u00abQuality Med\u00bb, Yekaterinburg 105318, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4409-2942","authenticated-orcid":false,"given":"Yuriy N.","family":"Davidyuk","sequence":"additional","affiliation":[{"name":"OpenLab \u201cGene and Cell Technologies\u201d, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana F.","family":"Khaiboullina","sequence":"additional","affiliation":[{"name":"OpenLab \u201cGene and Cell Technologies\u201d, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey P.","family":"Morzunov","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Nevada, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magasuba","family":"N\u2019Fally","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Pharmacy and Dentistry, University Gamal Abdel Nasser, Conakry 001, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanaba","family":"Bumbali","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology of Guinea, Kindia 100, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou Fode","family":"Camara","sequence":"additional","affiliation":[{"name":"Higher Institute of Science and Veterinary Medicine, Dalaba 280, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou Yero","family":"Boiro","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology of Guinea, Kindia 100, Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2577-0434","authenticated-orcid":false,"given":"Alexander P.","family":"Agafonov","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Gavrilova","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinat A.","family":"Maksyutov","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u00abVector\u00bb of Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,29]]},"reference":[{"key":"ref_1","unstructured":"Sykes, J.E., and Norris, J.M. (2014). Canine and Feline Infectious Diseases, Elsevier Inc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2692","DOI":"10.1128\/jcm.27.12.2692-2696.1989","article-title":"Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction","volume":"27","author":"Wilson","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/j.1574-6968.1993.tb06537.x","article-title":"Phylogenic homogeneity of Coxiella burnetii strains as determinated by 16S ribosomal RNA sequencing","volume":"113","author":"Stein","year":"1993","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e00096-20","DOI":"10.1128\/IAI.00096-20","article-title":"Coxiella burnetii Requires Host Eukaryotic Initiation Factor 2\u03b1 Activity for Efficient Intracellular Replication","volume":"88","author":"Brann","year":"2020","journal-title":"Infect. Immun."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ullah, Q., Jamil, T., Saqib, M., Iqbal, M., and Neubauer, H. (2022). Q Fever\u2014A Neglected Zoonosis. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081530"},{"key":"ref_6","first-page":"60","article-title":"Coxiella burnetii\u2014Pathogenic Agent of Q (Query) Fever","volume":"41","author":"Bauerfeind","year":"2014","journal-title":"Transfus. Med. Hemother. Off. Organ Der Dtsch. Fur Transfus. Und Immunhamatol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mediannikov, O., Fenollar, F., Socolovschi, C., Diatta, G., Bassene, H., Molez, J.F., Sokhna, C., Trape, J.F., and Raoult, D. (2010). Coxiella burnetii in humans and ticks in rural Senegal. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000654"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2478\/jvetres-2021-0055","article-title":"Coxiella burnetii DNA in Milk, Milk Products, and Fermented Dairy Products","volume":"65","author":"Valkovska","year":"2021","journal-title":"J. Vet. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/zph.12707","article-title":"Molecular detection of Coxiella burnetii in raw meat intended for pet consumption","volume":"67","author":"Shapiro","year":"2020","journal-title":"Zoonoses Public Health"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"754455","DOI":"10.3389\/fcimb.2021.754455","article-title":"Recent Advances on the Innate Immune Response to Coxiella burnetii","volume":"11","author":"Sireci","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.17116\/terarkh2015871184-91","article-title":"Description of a case of severe Q fever","volume":"87","author":"Malov","year":"2015","journal-title":"Ter. Arkhiv"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e181580","DOI":"10.1001\/jamanetworkopen.2018.1580","article-title":"Clinical Features and Complications of Coxiella burnetiid Infections From the French National Reference Center for Q Fever","volume":"1","author":"Melenotte","year":"2018","journal-title":"JAMA Netw. Open"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"925","DOI":"10.3201\/eid2006.130584","article-title":"Adverse pregnancy outcomes and Coxiella burnetii antibodies in pregnant women, Denmark","volume":"20","author":"Nielsen","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/CMR.12.4.518","article-title":"Q fever","volume":"12","author":"Maurin","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1068129","DOI":"10.3389\/fvets.2022.1068129","article-title":"Coxiella burnetii in ticks, livestock, pets and wildlife: A mini-review","volume":"9","author":"Celina","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13690-017-0248-y","article-title":"Farming, Q fever and public health: Agricultural practices and beyond","volume":"76","author":"Mori","year":"2018","journal-title":"Arch. Public Health"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1574-695X.2011.00876.x","article-title":"The 2007\u20132010 Q fever epidemic in the Netherlands: Characteristics of notified acute Q fever patients and the association with dairy goat farming","volume":"64","author":"Dijkstra","year":"2012","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.3201\/eid1709.110051","article-title":"Q Fever among Culling Workers, the Netherlands, 2009\u20132010","volume":"17","author":"Whelan","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.ijid.2019.10.043","article-title":"Epidemiological scenario of Q fever hospitalized patients in the Spanish Health System: What\u2019s new","volume":"90","author":"Almeida","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Clark, N.J., and Magalh\u00e3es, R.J.S. (2018). Airborne geographical dispersal of Q fever from livestock holdings to human communities: A systematic review and critical appraisal of evidence. BMC Infect. Dis., 18.","DOI":"10.1186\/s12879-018-3135-4"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Dedkov, V.G., Magassouba, N., Stukolova, O.A., Savina, V.A., Camara, J., Soropogui, B., Safonova, M.V., Semizhon, P., and Platonov, A.E. (2021). Differential Laboratory Diagnosis of Acute Fever in Guinea: Preparedness for the Threat of Hemorrhagic Fevers. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18116022"},{"key":"ref_22","unstructured":"(2023, April 26). World Health Organization, Available online: https:\/\/apps.who.int\/gho\/data\/node.main.688."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"770","DOI":"10.4269\/ajtmh.12-0580","article-title":"Coxiella burnetii in rodents on Heixiazi Island at the Sino-Russian border","volume":"88","author":"Liu","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_24","unstructured":"Walker, A.R., Bouattour, A., Camicas, J.-L., and Estrada-Pe\u00f1a, A. (2003). Ticks of Domestic Animals in Africa: A Guide to Identification of Species, Bioscience Reports."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Goheen, J.R., Palmer, T.M., Charles, G.K., Helgen, K.M., Kinyua, S.N., MacLean, J.E., Turner, B.L., Young, H.S., and Pringle, R.M. (2013). Piecewise Disassembly of a Large-Herbivore Community across a Rainfall Gradient: The UHURU Experiment. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055192"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.pt.2015.06.014","article-title":"The importance of ticks in Q fever transmission: What has (and has not) been demonstrated?","volume":"31","author":"Duron","year":"2015","journal-title":"Trends Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.2307\/4586287","article-title":"A Case of Q Fever Probably Contracted by Exposure to Ticks in Nature","volume":"62","author":"Eklund","year":"1947","journal-title":"Public Health Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1157\/13102278","article-title":"Fiebre Q tras picadura de garrapatas","volume":"25","year":"2007","journal-title":"Enferm. Infecc. Microbiol. Cl\u00edn."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1007\/s10096-014-2090-2","article-title":"Scalp eschar and neck lymphadenopathy after tick bite: An emerging syndrome with multiple causes","volume":"33","author":"Dubourg","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1111\/j.1445-5994.1989.tb00258.x","article-title":"Pericarditis associated with tick-borne Q fever","volume":"19","author":"Beaman","year":"1989","journal-title":"Aust. N. Z. J. Med."},{"key":"ref_31","first-page":"916142","article-title":"Q Fever with Unusual Exposure History: A Classic Presentation of a Commonly Misdiagnosed Disease","volume":"2012","author":"Nett","year":"2012","journal-title":"Case Rep. Infect. Dis."},{"key":"ref_32","first-page":"22","article-title":"Seroepidemiological and epizootological studies of tick-borne rickettsioses in the Republic of Guinea","volume":"1","author":"Buaro","year":"2013","journal-title":"Pest Manag."},{"key":"ref_33","unstructured":"Chief State Sanitary Physician of the Russian Federation Resolution (2014). Safety of Work with Microorganisms of Risk (Hazard) Groups 1 and 2, Chief state sanitary physician of the Russian Federation resolution. Sanitary Rules 1.3.3118-13."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"70","DOI":"10.36233\/0372-9311-2016-3-70-74","article-title":"Comparison of diagnostic effectiveness of methods of detection of Coxiella burnetii in blood of patients with Q fever based on amplification of 16s rRNA gene fragments (standard PCR) and groel gene (realtime PCR)","volume":"93","author":"Panferova","year":"2016","journal-title":"J. Microbiol. Epidemiol. Immunobiol."},{"key":"ref_35","unstructured":"Freilikhman, O.A., Tokarevich, N.K., and Panferova, Y.A. (2012). Kit for Detection of cu Fever Pathogen in Biological Material by Real-Time Polymerase Chain Reaction (PCR-PV). (RU2525059C2)."},{"key":"ref_36","unstructured":"(2023, April 28). AmpliSence Coxiella burnetii-FL. Detection of the Causative Agent of Q Fever (Coxiella burnetii). Available online: https:\/\/www.amplisens.ru\/upload\/iblock\/352\/Coxiella%20burnetii-FL.pdf."},{"key":"ref_37","unstructured":"Dedu, I. (1989). Ecological Encyclopedic Dictionary, Main Editorial Board of the Moldavian Soviet Encyclopedia (ITU)."},{"key":"ref_38","unstructured":"Centers of the State Sanitary and Epidemiological Surveillance of the Russian Federation (2019). Collecting, Recording and Preparing for Laboratory Examination of Blood-Sicking Arthropod Vectors of Natural Focal Pathogens, Centers of the State Sanitary and Epidemiological Surveillance of the Russian Federation. Methodological Guidelines 3.1.1027-01."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"165","DOI":"10.15789\/2220-7619-NDO-1485","article-title":"New data on the level of immune stratum against Q fever agent in population of the of Republic of Guinea","volume":"11","author":"Naydenova","year":"2021","journal-title":"Russ. J. Infect. Immun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7","DOI":"10.15789\/2220-7619-2016-1-7-24","article-title":"Coxiella burnetii pathogenicity molecular basis","volume":"6","author":"Panferova","year":"2016","journal-title":"Russ. J. Infect. Immun. Infektsiyaiimmun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s13071-020-3956-z","article-title":"Uptake and fecal excretion of Coxiella burnetii by Ixodes ricinus and Dermacentor marginatus ticks","volume":"13","author":"Makert","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ttbdis.2012.02.003","article-title":"Coxiella symbiont in the tick Ornithodoros rostratus (Acari: Argasidae)","volume":"3","author":"Almeida","year":"2012","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.ttbdis.2014.04.003","article-title":"Diversity and global distribution of the Coxiella intracellular bacterium in seabird ticks","volume":"5","author":"Duron","year":"2014","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0304-4017(84)90082-7","article-title":"Seasonal pattern of tick load in Bunaji cattle in the subhumid zone of Nigeria","volume":"15","author":"Bayer","year":"1984","journal-title":"Vet. Parasitol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Duron, O., No\u00ebl, V., McCoy, K.D., Bonazzi, M., Sidi-Boumedine, K., Morel, O., Vavre, F., Zenner, L., Jourdain, E., and Durand, P. (2015). The Recent Evolution of a Maternally-Inherited Endosymbiont of Ticks Led to the Emergence of the Q Fever Pathogen, Coxiella burnetii. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004892"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1093\/gbe\/evv016","article-title":"A Coxiella-Like Endosymbiont Is a Potential Vitamin Source for the Lone Star Tick","volume":"7","author":"Smith","year":"2015","journal-title":"Genome Biol. Evol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1128\/AAC.01424-08","article-title":"Bacteriostatic and Bactericidal Activities of Tigecycline against Coxiella burnetii and Comparison with Those of Six Other Antibiotics","volume":"53","author":"Spyridaki","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Troupin, C., Ellis, I., Doukour\u00e9, B., Camara, A., Keita, M., Kagbadouno, M., Bart, J.-M., Diallo, R., Lac\u00f4te, S., and Marianneau, P. (2022). Seroprevalence of brucellosis, Q fever and Rift Valley fever in domestic ruminants in Guinea in 2017\u20132019. BMC Vet Res., 18.","DOI":"10.1186\/s12917-022-03159-x"},{"key":"ref_49","unstructured":"Balashov, Y.S., and Daiter, A.B. (1973). Bloodsucking arthropods and rickettsiae. Sci. Leningr., 251."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"655715","DOI":"10.3389\/fvets.2021.655715","article-title":"The Prevalence of Coxiella burnetii in Hard Ticks in Europe and Their Role in Q Fever Transmission Revisited\u2014A Systematic Review","volume":"8","author":"Makert","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ni, J., Lin, H., Xu, X., Ren, Q., Aizezi, M., Luo, J., Luo, Y., Ma, Z., Chen, Z., and Tan, Y. (2020). Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang areas of China. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02538-6"},{"key":"ref_52","first-page":"551","article-title":"Prevalence of Coxiella burnetii (Legionellales: Coxiellaceae) Infection among Wildlife Species and the Tick Hyalomma lusitanicum (Acari: Ixodidae) in a Meso-Mediterranean Ecosystem","volume":"57","author":"Tercero","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"723","DOI":"10.5604\/12321966.1129922","article-title":"Prevalence of tick-borne pathogens in an urban park in Rome, Italy","volume":"21","author":"Mancini","year":"2014","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"101926","DOI":"10.1016\/j.ttbdis.2022.101926","article-title":"Prevalence of Coxiella-infections in ticks\u2014Review and meta-analysis","volume":"13","author":"Yessinou","year":"2022","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.nmni.2018.08.017","article-title":"Prevalence of Anaplasma phagocytophilum and Coxiella burnetii in Ixodes ricinus ticks in Switzerland: An underestimated epidemiologic risk","volume":"27","author":"Pilloux","year":"2018","journal-title":"New Microbes New Infect."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Knap, N., \u017dele, D., Bi\u0161kup, U.G., Av\u0161i\u010d-\u017dupanc, T., and Vengu\u0161t, G. (2019). The prevalence of Coxiella burnetii in ticks and animals in Slovenia. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2130-3"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e01270","DOI":"10.1016\/j.heliyon.2019.e01270","article-title":"Detection of pathogens in Dermacentor reticulatus in northwestern Europe: Evaluation of a high-throughput array","volume":"5","author":"Sprong","year":"2019","journal-title":"Heliyon"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s10493-006-9012-7","article-title":"Borrelia, Coxiella, and Rickettsia in Carios capensis (Acari: Argasidae) from a brown pelican (Pelecanus occidentalis) rookery in South Carolina, USA","volume":"39","author":"Reeves","year":"2006","journal-title":"Exp. Appl. Acarol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.ttbdis.2019.03.014","article-title":"Tissue localization of Coxiella-like endosymbionts in three European tick species through fluorescence in situ hybridization","volume":"10","author":"Buysse","year":"2019","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1093\/gbe\/evv108","article-title":"Distinctive Genome Reduction Rates Revealed by Genomic Analyses of Two Coxiella-Like Endosymbionts in Ticks","volume":"7","author":"Gottlieb","year":"2015","journal-title":"Genome Biol. Evol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Klee, S.R., Tyczka, J., Ellerbrok, H., Franz, T., Linke, S., Baljer, G., and Appel, B. (2006). Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol., 6.","DOI":"10.1186\/1471-2180-6-2"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1128\/JCM.41.5.1869-1874.2003","article-title":"Evaluation of Coxiella burnetii Antibiotic Susceptibilities by Real-Time PCR Assay","volume":"41","author":"Brennan","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1128\/jcm.30.9.2462-2466.1992","article-title":"Detection of Coxiella burnetii by DNA amplification using polymerase chain reaction","volume":"30","author":"Stein","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"fnv132","DOI":"10.1093\/femsle\/fnv132","article-title":"The IS1111 insertion sequence used for detection of Coxiella burnetii is widespread in Coxiella-like endosymbionts of ticks","volume":"362","author":"Duron","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_65","first-page":"29230","article-title":"Molecular methods routinely used to detect Coxiella burnetii in ticks cross-react with Coxiella-like bacteria","volume":"5","author":"Jourdain","year":"2015","journal-title":"Infect. Ecol. Epidemiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"698395","DOI":"10.3389\/fvets.2021.698395","article-title":"Predictive Analytics of Cattle Host and Environmental and Micro-Climate Factors for Tick Distribution and Abundance at the Livestock\u2013Wildlife Interface in the Lower Okavango Delta of Botswana","volume":"8","author":"Babayani","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s11250-010-9690-3","article-title":"Tick loads in cattle raised on sweet and sour rangelands in the low-input farming areas of South Africa","volume":"43","author":"Marufu","year":"2011","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Faccini, J.L.H., De Almeida, T.K., Sousa, I.C., Costa, L.M., and Luz, H.R. (2021). Temperature effects on the non-parasitic phase of Amblyomma parvum (Acari:Ixodidae) Syst. Appl. Acar., 1168\u20131176.","DOI":"10.11158\/saa.26.6.12"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.14202\/vetworld.2022.1943-1953","article-title":"Prevalence and tick loads in Nguni cattle reared in different environmental conditions across four provinces of South Africa","volume":"15","author":"Mapholi","year":"2022","journal-title":"Vet. World"},{"key":"ref_70","first-page":"e00832","article-title":"Association of ticks with seasons, age, and cattle color of North-Western region of Nigeria","volume":"12","author":"Okwuonu","year":"2021","journal-title":"Sci. Afr."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1186\/s13071-021-04683-9","article-title":"Interactive effects of biological, human and environmental factors on tick loads in Boran cattle in tropical drylands","volume":"14","author":"Chepkwony","year":"2021","journal-title":"Parasit Vectors"},{"key":"ref_72","unstructured":"Russia Federal Service for Supervision in the field of consumer rights protection and human well-being (hereinafter\u2014Rospotrebnadzor) (2010). Prevention of Coxiellosis (Q Fever), Russia Federal Service for Supervision in the field of consumer rights protection and human well-being (hereinafter\u2014Rospotrebnadzor). Sanitary Rules 3.1.7.2811-10."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"20170475","DOI":"10.1098\/rspb.2017.0475","article-title":"Interacting effects of wildlife loss and climate on ticks and tick-borne disease","volume":"284","author":"Titcomb","year":"2017","journal-title":"Proc. R. Soc. B Biol. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s10493-013-9671-0","article-title":"Efficiency of flagging and dragging for tick collection","volume":"61","author":"Lia","year":"2013","journal-title":"Exp. Appl. Acarol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1365-2915.1993.tb00684.x","article-title":"Climate, satellite imagery and the seasonal abundance of the tick Rhipicephalus appendiculatus in southern Africa: A new perspective","volume":"7","author":"Randolph","year":"1993","journal-title":"Med. Vet. E\u00c8ntomol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1890\/0012-9658(2002)083[0255:CCAVAL]2.0.CO;2","article-title":"Comparing Climate and Vegetation as Limiting Factors for Species Ranges of African Ticks","volume":"83","author":"Cumming","year":"2002","journal-title":"Ecology"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1175\/1520-0442(2002)015<1002:TEAMMR>2.0.CO;2","article-title":"The East African March\u2013May rainy season: Associated atmospheric dynamics and predictability over the 1968\u20131997 period","volume":"15","author":"Camberlin","year":"2002","journal-title":"J. Clim."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/6\/1433\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:44:20Z","timestamp":1760125460000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/6\/1433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,29]]},"references-count":77,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["microorganisms11061433"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11061433","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,29]]}}}