{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T17:47:06Z","timestamp":1786297626673,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNRS","award":["University Paris-Saclay"],"award-info":[{"award-number":["University Paris-Saclay"]}]},{"name":"CNRS","award":["11-BTBR-0003 (PROBIO3)"],"award-info":[{"award-number":["11-BTBR-0003 (PROBIO3)"]}]},{"name":"CNRS","award":["ANR-15-CE09-0002"],"award-info":[{"award-number":["ANR-15-CE09-0002"]}]},{"name":"CNRS","award":["ANR-17-ASTR-0018 INNOVANTIBIO"],"award-info":[{"award-number":["ANR-17-ASTR-0018 INNOVANTIBIO"]}]},{"name":"\u201cInvestment for the Future: Biotechnologies et Bioressources\u201d","award":["University Paris-Saclay"],"award-info":[{"award-number":["University Paris-Saclay"]}]},{"name":"\u201cInvestment for the Future: Biotechnologies et Bioressources\u201d","award":["11-BTBR-0003 (PROBIO3)"],"award-info":[{"award-number":["11-BTBR-0003 (PROBIO3)"]}]},{"name":"\u201cInvestment for the Future: Biotechnologies et Bioressources\u201d","award":["ANR-15-CE09-0002"],"award-info":[{"award-number":["ANR-15-CE09-0002"]}]},{"name":"\u201cInvestment for the Future: Biotechnologies et Bioressources\u201d","award":["ANR-17-ASTR-0018 INNOVANTIBIO"],"award-info":[{"award-number":["ANR-17-ASTR-0018 INNOVANTIBIO"]}]},{"name":"ANR BioSound-IR","award":["University Paris-Saclay"],"award-info":[{"award-number":["University Paris-Saclay"]}]},{"name":"ANR BioSound-IR","award":["11-BTBR-0003 (PROBIO3)"],"award-info":[{"award-number":["11-BTBR-0003 (PROBIO3)"]}]},{"name":"ANR BioSound-IR","award":["ANR-15-CE09-0002"],"award-info":[{"award-number":["ANR-15-CE09-0002"]}]},{"name":"ANR BioSound-IR","award":["ANR-17-ASTR-0018 INNOVANTIBIO"],"award-info":[{"award-number":["ANR-17-ASTR-0018 INNOVANTIBIO"]}]},{"name":"\u201cDirection G\u00e9n\u00e9rale des Arm\u00e9es\u201d (DGA)","award":["University Paris-Saclay"],"award-info":[{"award-number":["University Paris-Saclay"]}]},{"name":"\u201cDirection G\u00e9n\u00e9rale des Arm\u00e9es\u201d (DGA)","award":["11-BTBR-0003 (PROBIO3)"],"award-info":[{"award-number":["11-BTBR-0003 (PROBIO3)"]}]},{"name":"\u201cDirection G\u00e9n\u00e9rale des Arm\u00e9es\u201d (DGA)","award":["ANR-15-CE09-0002"],"award-info":[{"award-number":["ANR-15-CE09-0002"]}]},{"name":"\u201cDirection G\u00e9n\u00e9rale des Arm\u00e9es\u201d (DGA)","award":["ANR-17-ASTR-0018 INNOVANTIBIO"],"award-info":[{"award-number":["ANR-17-ASTR-0018 INNOVANTIBIO"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Streptomyces coelicolor M145 is a model strain extensively studied to elucidate the regulation of antibiotic biosynthesis in Streptomyces species. This strain abundantly produces the blue polyketide antibiotic, actinorhodin (ACT), and has a low lipid content. In a process designed to delete the gene encoding the isocitrate lyase (sco0982) of the glyoxylate cycle, an unexpected variant of S. coelicolor was obtained besides bona fide\u00a0sco0982 deletion mutants. This variant produces 7- to 15-fold less ACT and has a 3-fold higher triacylglycerol and phosphatidylethanolamine content than the original strain. The genome of this variant was sequenced and revealed that 704 genes were deleted (9% of total number of genes) through deletions of various sizes accompanied by the massive loss of mobile genetic elements. Some deletions include genes whose absence could be related to the high total lipid content of this variant such as those encoding enzymes of the TCA and glyoxylate cycles, enzymes involved in nitrogen assimilation as well as enzymes belonging to some polyketide and possibly trehalose biosynthetic pathways. The characteristics of this deleted variant of S. coelicolor are consistent with the existence of the previously reported negative correlation existing between lipid content and antibiotic production in Streptomyces species.<\/jats:p>","DOI":"10.3390\/microorganisms11061470","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T01:40:56Z","timestamp":1685583656000},"page":"1470","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics"],"prefix":"10.3390","volume":"11","author":[{"given":"Thierry","family":"Dulermo","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Institute for Integrative Biology of the Cell (I2BC), Department of Microbiology, Group \u201cEnergetic Metabolism of <i>Streptomyces<\/i>\u201d, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Lejeune","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Institute for Integrative Biology of the Cell (I2BC), Department of Microbiology, Group \u201cEnergetic Metabolism of <i>Streptomyces<\/i>\u201d, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ece","family":"Aybeke","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Institut de Chimie Physique, UMR 8000, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7236-2292","authenticated-orcid":false,"given":"Sonia","family":"Abreu","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Lip(Sys)2 (Lipides Syst\u00e8mes Analytiques et Biologiques), UFR Pharmacie\u2014B\u00e2timent Henri Moissan, 17 Avenue des Sciences, 91400 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Bleton","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Lip(Sys)2 (Lipides Syst\u00e8mes Analytiques et Biologiques), UFR Pharmacie\u2014B\u00e2timent Henri Moissan, 17 Avenue des Sciences, 91400 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"David","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Institute for Integrative Biology of the Cell (I2BC), Department of Microbiology, Group \u201cEnergetic Metabolism of <i>Streptomyces<\/i>\u201d, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1923-4988","authenticated-orcid":false,"given":"Ariane","family":"Deniset-Besseau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Institut de Chimie Physique, UMR 8000, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Chaminade","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Lip(Sys)2 (Lipides Syst\u00e8mes Analytiques et Biologiques), UFR Pharmacie\u2014B\u00e2timent Henri Moissan, 17 Avenue des Sciences, 91400 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabelle","family":"Thibessard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, INRAE, DynAMic, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8703-454X","authenticated-orcid":false,"given":"Pierre","family":"Leblond","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, INRAE, DynAMic, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Joelle","family":"Virolle","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CNRS, CEA, Institute for Integrative Biology of the Cell (I2BC), Department of Microbiology, Group \u201cEnergetic Metabolism of <i>Streptomyces<\/i>\u201d, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/417141a","article-title":"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)","volume":"417","author":"Bentley","year":"2002","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/B978-0-12-800259-9.00006-8","article-title":"Production of specialized metabolites by Streptomyces coelicolor A3(2)","volume":"89","author":"Dyson","year":"2014","journal-title":"Adv. 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