{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:44:33Z","timestamp":1792014273272,"version":"4.4.0"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Breast cancer is one of the leading causes of death in women worldwide. Recent studies have demonstrated that inflammation due to infections with microorganisms could play a role in breast cancer development. One of the known human pathogens, Borrelia burgdorferi, the causative agent of Lyme disease, has been shown to be present in various types of breast cancer and is associated with poor prognosis. We reported that B. burgdorferi can invade breast cancer cells and affect their tumorigenic phenotype. To better understand the genome-wide genetic changes caused by B. burgdorferi, we evaluated the microRNA (miRNA or miR) expression profiles of two triple-negative breast cancer cell lines and one non-tumorigenic mammary cell line before and after B. burgdorferi infection. Using a cancer-specific miRNA panel, four miRNAs (miR-206, 214-3p, 16-5p, and 20b-5p) were identified as potential markers for Borrelia-induced changes, and the results were confirmed by quantitative real-time reverse transcription (qRT-PCR). Among those miRNAs, miR-206 and 214 were the most significantly upregulated miRNAs. The cellular impact of miR-206 and 214 was evaluated using DIANA software to identify related molecular pathways and genes. Analyses showed that the cell cycle, checkpoints, DNA damage\u2013repair, proto-oncogenes, and cancer-related signaling pathways are mostly affected by B. burgdorferi infection. Based on this information, we have identified potential miRNAs which could be further evaluated as biomarkers for tumorigenesis caused by pathogens in breast cancer cells.<\/jats:p>","DOI":"10.3390\/microorganisms11061475","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T01:40:56Z","timestamp":1685583656000},"page":"1475","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Borrelia burgdorferi on the Expression of miRNAs in Breast Cancer and Normal Mammary Epithelial Cells"],"prefix":"10.3390","volume":"11","author":[{"given":"Ananya","family":"Debbarma","sequence":"first","affiliation":[{"name":"Lyme Disease Research Group, Department of Biology and Environmental Science, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miranda","family":"Mansolf","sequence":"additional","affiliation":[{"name":"Lyme Disease Research Group, Department of Biology and Environmental Science, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishwa A.","family":"Khatri","sequence":"additional","affiliation":[{"name":"Lyme Disease Research Group, Department of Biology and Environmental Science, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine A.","family":"Valentino","sequence":"additional","affiliation":[{"name":"Lyme Disease Research Group, Department of Biology and Environmental Science, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Sapi","sequence":"additional","affiliation":[{"name":"Lyme Disease Research Group, Department of Biology and Environmental Science, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"951","DOI":"10.3389\/fmicb.2018.00951","article-title":"Distinct microbial signatures associated with different breast cancer types","volume":"9","author":"Banerjee","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.7150\/ijbs.21635","article-title":"Risk factors and preventions of breast cancer","volume":"13","author":"Sun","year":"2017","journal-title":"Int. J. Biol. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1093\/jnci\/91.11.943","article-title":"Prevalence of BRCA1 and BRCA2 gene mutations in patients with early-onset breast cancer","volume":"91","author":"Peto","year":"1999","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1159\/000369571","article-title":"Influence of lifestyle factors on breast cancer risk","volume":"9","author":"Dieterich","year":"2014","journal-title":"Breast Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1146\/annurev-publhealth-040617-014101","article-title":"Environmental Determinants of Breast Cancer","volume":"39","author":"Hiatt","year":"2018","journal-title":"Annu. Rev. Public Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.aaa4972","article-title":"Cancer and the microbiota","volume":"348","author":"Garrett","year":"2015","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Azevedo, M.M., Pina-Vaz, C., and Baltazar, F. (2020). Microbes and Cancer: Friends or Faux?. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093115"},{"key":"ref_8","first-page":"955","article-title":"Epidemiological ins and outs of Helicobacter pylori: A review","volume":"62","author":"Muhammad","year":"2012","journal-title":"J. Pak. Med. Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e12405","DOI":"10.1111\/hel.12405","article-title":"Pathogenesis of Helicobacter pylori infection","volume":"22","author":"Camilo","year":"2017","journal-title":"Helicobacter"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.3892\/ol.2017.6388","article-title":"Microbial infection, inflammation, and epithelial ovarian cancer","volume":"14","author":"Xie","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1007\/s10096-012-1570-5","article-title":"Viral and bacterial etiologies of epithelial ovarian cancer","volume":"31","author":"Shanmughapriya","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Xu, S., Yin, W., Zhang, Y., Lv, Q., Yang, Y., and He, J. (2020). Foes or Friends? Bacteria enriched in the tumor microenvironment of colorectal cancer. Cancers, 12.","DOI":"10.3390\/cancers12020372"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4724","DOI":"10.1038\/ncomms5724","article-title":"Microbial genomic analysis reveals the essential role of inflammation in bacteria-induced colorectal cancer","volume":"5","author":"Arthur","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1038\/s41467-020-16967-2","article-title":"Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression","volume":"11","author":"Parhi","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.cell.2017.07.008","article-title":"Fusobacterium nucleatum promotes chemoresistance to colorectal cancer by modulating autophagy","volume":"170","author":"Yu","year":"2017","journal-title":"Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1016\/j.cell.2022.02.027","article-title":"Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer","volume":"185","author":"Fu","year":"2022","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5039","DOI":"10.1128\/AEM.01235-16","article-title":"The microbiota of breast tissue and its association with breast cancer","volume":"82","author":"Urbaniak","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","first-page":"61","article-title":"Overview of the clinical manifestations of Borrelia burgdorferi infection","volume":"2","author":"Dattwyler","year":"1991","journal-title":"Can. J. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1016\/j.micinf.2006.08.014","article-title":"Invasion of human neuronal and glial cells by an infectious strain of Borrelia burgdorferi","volume":"8","author":"Livengood","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gaur, G., Sawant, J.Y., Chavan, A.S., Khatri, V.A., Liu, Y.-H., Zhang, M., and Sapi, E. (2021). Effect of invasion of Borrelia burgdorferi in normal and neoplastic mammary epithelial cells. Antibiotics, 10.","DOI":"10.3390\/antibiotics10111295"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/nrc2867","article-title":"Genetic variation in microRNA networks: The implications for cancer research","volume":"10","author":"Ryan","year":"2010","journal-title":"Nat. Rev. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5336","DOI":"10.2174\/1381612820666140128204914","article-title":"MiRNAs in human cancers: The diagnostic and therapeutic implications","volume":"20","author":"Wu","year":"2014","journal-title":"Curr. Pharm. Des."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.molmed.2014.06.005","article-title":"MicroRNAs in cancer: Biomarkers, functions and therapy","volume":"20","author":"Hayes","year":"2014","journal-title":"Trends Mol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lochhead, R.B., Ma, Y., Zachary, J.F., Baltimore, D., Zhao, J.L., Weis, J.H., O\u2019Connell, R.M., and Weis, J.J. (2014). MicroRNA-146a provides feedback regulation of Lyme arthritis but not carditis during infection with Borrelia burgdorferi. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1004212"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1002\/art.40039","article-title":"MicroRNA expression shows inflammatory dysregulation and tumor like proliferative responses in joints of patients with postinfectious Lyme arthritis","volume":"69","author":"Lochhead","year":"2017","journal-title":"Arthritis Rheumatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"W460","DOI":"10.1093\/nar\/gkv403","article-title":"DIANA-miRPath v3.0, deciphering microRNA function with experimental support","volume":"43","author":"Vlachos","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ligasov\u00e1, A., and Koberna, K. (2021). Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910759"},{"key":"ref_28","first-page":"6638","article-title":"Expression of miRNA-206 and miRNA-145 in breast cancer and correlation with prognosis","volume":"16","author":"Quan","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_29","first-page":"4035","article-title":"Inhibition of miR 214 attenuates the migration and invasion of triple-negative Breast Cancer cells","volume":"19","author":"Zhang","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wang, Z., Hu, S., Li, X., Liu, Z., Han, D., Wang, Y., Wei, L., Zhang, G., and Wang, X. (2021). MiR-16-5p suppresses breast cancer proliferation by targeting ANLN. BMC Cancer, 21.","DOI":"10.1186\/s12885-021-08914-1"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Xia, L., Li, F., Qiu, J., Feng, Z., Xu, Z., Chen, Z., and Sun, J. (2020). Oncogenic miR-20b-5p contributes to malignant behaviors of breast cancer stem cells by bidirectionally regulating CCND1 and E2F1. BMC Cancer, 20.","DOI":"10.1186\/s12885-020-07395-y"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"945","DOI":"10.3389\/fneur.2019.00945","article-title":"MMP-9-Related microRNAs as Prognostic Markers for Hemorrhagic Transformation in Cardioembolic Stroke Patients","volume":"10","author":"Zheng","year":"2019","journal-title":"Front. Neurol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.bbrc.2016.06.038","article-title":"MicroRNA-206 regulates the secretion of inflammatory cytokines and MMP9 expression by targeting TIMP3 in Mycobacterium tuberculosis-infected THP-1 human macrophages","volume":"477","author":"Fu","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wright, K., de Silva, K., Plain, K.M., Purdie, A.C., Blair, T.A., Duggin, I.G., Britton, W.J., and Oehlers, S.H. (2021). Mycobacterial infection-induced miR-206 inhibits protective neutrophil recruitment via the CXCL12\/CXCR4 signaling Chang axis. PLoS Pathog., 17.","DOI":"10.1101\/2020.12.14.422665"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Casselli, T., Qureshi, H., Peterson, E., Perley, D., Blake, E., Jokinen, B., Abbas, A., Nechaev, S., Watt, J.A., and Dhasarathy, A. (2017). MicroRNA and mRNA transcriptome profiling in primary human astrocytes infected with Borrelia burgdorferi. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0170961"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Cui, L., Markou, A., Stratton, C.W., and Lianidou, E. (2018). Diagnosis and Assessment of Microbial Infections with Host and Microbial MicroRNA Profiles. Adv. Tech. Diagn. Microbiol., 563\u2013597.","DOI":"10.1007\/978-3-319-95111-9_23"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/2045-3701-4-62","article-title":"MicroRNA-20b promotes cell growth of breast cancer cells partly via targeting phosphatase and tensin homologue (PTEN)","volume":"4","author":"Zhou","year":"2014","journal-title":"Cell Biosci."},{"key":"ref_38","unstructured":"Wong, L., Lee, K., Russell, I., and Chen, C. (2022, December 12). Endogenous Controls for Real-Time Quantitation of miRNA Using TaqMan\u00ae MicroRNA Assays. Applied Biosystems Application Note, Publication 127AP11-01. Available online: https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/Application-Notes\/cms_044972.pdf."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"41127","DOI":"10.1038\/srep41127","article-title":"Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation","volume":"7","author":"Grasso","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1111\/jcmm.12927","article-title":"Identification of stably expressed reference small non-coding RNAs for microRNA quantification in high-grade serous ovarian carcinoma tissues","volume":"20","author":"Bignotti","year":"2016","journal-title":"J. Cell. Mol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1533033819875168","DOI":"10.1177\/1533033819875168","article-title":"MiR-206 Promotes Cancer Progression by Targeting Full-Length Neurokinin-1 Receptor in Breast Cancer","volume":"18","author":"Zhou","year":"2019","journal-title":"Technol. Cancer Res. Treat."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Limana, F., Esposito, G., D\u2019Arcangelo, D., Di Carlo, A., Romani, S., Melillo, G., Mangoni, A., Bertolami, C., Pompilio, G., and Germani, A. (2011). HMGB1 attenuates cardiac remodeling in the failing heart via enhanced cardiac regeneration and miR-206-mediated inhibition of TIMP-3. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0019845"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s13053-015-0028-z","article-title":"High expression of miR-214 is associated with a worse disease-specific survival of the triple-negative breast cancer patients","volume":"13","author":"Kalniete","year":"2015","journal-title":"Hered. Cancer Clin. Pract."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1007\/s10616-019-00352-z","article-title":"MiR-214-3p regulates the viability, invasion, migration and EMT of TNBC cells by targeting ST6GAL1","volume":"71","author":"Tao","year":"2019","journal-title":"Cytotechnology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/j.bbrc.2017.03.167","article-title":"Differential expression of ST6GAL1 in the tumor progression of colorectal cancer","volume":"486","author":"Zhang","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1186\/s13046-019-1340-7","article-title":"ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p\/LASP1 signaling axis","volume":"38","author":"Wang","year":"2019","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"663","DOI":"10.2147\/CMAR.S162813","article-title":"ANLN functions as a key candidate gene in cervical cancer as determined by integrated bioinformatic analysis","volume":"10","author":"Xia","year":"2018","journal-title":"Cancer Manag. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1086\/605443","article-title":"Induction of microRNA-155 during Helicobacter pylori infection and its negative regulatory role in the inflammatory response","volume":"200","author":"Xiao","year":"2009","journal-title":"J. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1146\/annurev.pathol.4.110807.092311","article-title":"PTEN and the PI3-kinase pathway in cancer","volume":"4","author":"Chalhoub","year":"2009","journal-title":"Annu. Rev. Pathol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5120","DOI":"10.1038\/s41467-020-18794-x","article-title":"Concepts of extracellular matrix remodeling in tumor progression and metastasis","volume":"11","author":"Winkler","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Provenzano, P.P., Inman, D.R., Eliceiri, K.W., Knittel, J.G., Yan, L., Rueden, C.T., White, J.G., and Keely, P.J. (2008). Collagen density promotes mammary tumor initiation and progression. BMC Med., 6.","DOI":"10.1186\/1741-7015-6-11"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"a002576","DOI":"10.1101\/cshperspect.a002576","article-title":"Gap junctions","volume":"1","author":"Goodenough","year":"2009","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"913659","DOI":"10.3389\/fgene.2022.913659","article-title":"FN1 overexpression is correlated with unfavorable prognosis and immune infiltrates in breast cancer","volume":"13","author":"Zhang","year":"2022","journal-title":"Front. Genet."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s13023-018-0973-5","article-title":"Aberrant expressions of miRNA-206 target, FN1, in multifactorial Hirschsprung disease","volume":"14","author":"Gunadi","year":"2019","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"18803","DOI":"10.1074\/jbc.M501731200","article-title":"Borrelia burgdorferi binds fibronectin through a tandem beta-zipper, a common mechanism of fibronectin binding in staphylococci, streptococci, and spirochetes","volume":"280","author":"Raibaud","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"760627","DOI":"10.3389\/fmicb.2021.760627","article-title":"Comprehensive mapping of the cell response to Borrelia bavariensis in the brain microvascular endothelial cells in vitro using RNA-Seq","volume":"12","author":"Bhide","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/s13045-020-00959-2","article-title":"A WEE1 family business: Regulation of mitosis, cancer progression, and therapeutic target","volume":"13","author":"Cerchione","year":"2020","journal-title":"J. Hematol. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"135","DOI":"10.21037\/atm-20-7922","article-title":"Elevated EXO1 expression is associated with breast carcinogenesis and poor prognosis","volume":"9","author":"Liu","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1073\/pnas.1523693113","article-title":"Pten loss promotes MAPK pathway dependency in HER2\/neu breast carcinomas","volume":"113","author":"Ebbesen","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1093\/jmcb\/mjx007","article-title":"The role of MDM2 and MDM4 in breast cancer development and prevention","volume":"9","author":"Haupt","year":"2017","journal-title":"J. Mol. Cell. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e1481814","DOI":"10.1080\/23723556.2018.1481814","article-title":"CDC25 as a common therapeutic target for triple-negative breast cancer\u2014The challenges ahead","volume":"5","author":"Zacksenhaus","year":"2018","journal-title":"Mol. Cell. Oncol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s12964-020-0527-z","article-title":"Role of STAT3 signaling pathway in breast cancer","volume":"18","author":"Ma","year":"2020","journal-title":"Cell. Commun. Signal."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/bcr426","article-title":"The p53 pathway in breast cancer","volume":"4","author":"Gasco","year":"2002","journal-title":"Breast Cancer Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1515\/bmc-2013-0027","article-title":"The MDM2 gene family","volume":"5","author":"Mendoza","year":"2014","journal-title":"Biomol. Concepts."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/6\/1475\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:46:48Z","timestamp":1760125608000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/6\/1475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,1]]},"references-count":64,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["microorganisms11061475"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11061475","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,1]]}}}