{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:32:07Z","timestamp":1789464727699,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Veterinary Sciences Brno","award":["2021ITA26"],"award-info":[{"award-number":["2021ITA26"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Lyme disease, caused by some strains of bacterial spirochetes Borrelia burgdorferi sensu lato (Bbsl), affects humans but also domestic animals including horses. The primary pathogens in horses in Europe are B.\u00a0afzelii, B.\u00a0garinii and B. burgdorferi sensu stricto. To our knowledge, there are no data available on the seropositivity of B. burgdorferi s.l. in horses from the Czech Republic. In this country, horses are mainly used for sport, breeding, and recreational riding in areas where vectors of B. burgdorferi s.l. are present, which is why they are frequently at risk of infection. The aim of the study was to detect anti-borrelia IgM and IgG antibodies in clinically healthy and sick horses from the Czech Republic and to evaluate the risk factors of infection. In total, sera of 262 horses (247 clinically healthy horses and 15 horses hospitalized due to symptoms of encephalitis\/meningoencephalitis) were examined by an indirect sandwich enzyme-linked immunosorbent assay. Positivity of B.\u00a0burgdorferi was 27% (66\/247) in clinically healthy horses (21% IgM, 7% IgG and 3% IgM + IgG antibodies) and 20% (3\/15) in horses with clinical signs (20% IgM, 7% IgG and 7% IgM + IgG). In the clinically healthy horses, positivity statistically differed (p \u2264 0.05) only in Pony and Warmblood breeds, being the most affected at 32% and 30%, respectively, while other characteristics (sex, age, usage and localities) had no effect on positivity. This is the first survey of antibodies to B.\u00a0burgdorferi s.l. in Czech horses showing that horses are exposed to ticks infected with B.\u00a0burgdorferi s.l. This should be taken into account when making differential diagnoses in patients with non-specific symptoms to start with adequate therapy.<\/jats:p>","DOI":"10.3390\/microorganisms11071706","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:51:06Z","timestamp":1688086266000},"page":"1706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibodies against Borrelia burgdorferi Sensu Lato in Clinically Healthy and Sick Horses: First Report from the Czech Republic"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7079-5169","authenticated-orcid":false,"given":"Nikola","family":"Ka\u0161p\u00e1rkov\u00e1","sequence":"first","affiliation":[{"name":"Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palack\u00e9ho t\u0159. 1946\/1, 612 42 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9087-0880","authenticated-orcid":false,"given":"Eva","family":"B\u00e1rtov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palack\u00e9ho t\u0159. 1946\/1, 612 42 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3382-0913","authenticated-orcid":false,"given":"Alena","family":"\u017d\u00e1kovsk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic"},{"name":"Department of Biology, Faculty of Education, Masaryk University, Po\u0159\u00ed\u010d\u00ed 7\/9, 625 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Bud\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Statistics, Faculty of Science, Masaryk University, Kotl\u00e1\u0159sk\u00e1 267\/2, 611 37 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil","family":"Sedl\u00e1k","sequence":"additional","affiliation":[{"name":"State Veterinary Institute Prague, S\u00eddli\u0161tn\u00ed 136\/24, 165 03 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1186\/ar2853","article-title":"Treatment of Lyme borreliosis","volume":"11","author":"Girschick","year":"2009","journal-title":"Arthritis Res. 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