{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:24:17Z","timestamp":1788866657388,"version":"build-2803163510"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USDA Agricultural Research Service","award":["# 58-6040-8-034"],"award-info":[{"award-number":["# 58-6040-8-034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Necrotic enteritis (NE) is an intestinal disease that results in poor performance, inefficient nutrient absorption, and has a devastating economic impact on poultry production. This study evaluated the effects of a saponin-based product (Clarity Q, CQ) during an NE challenge. A total of 1200 male chicks were randomly assigned to four dietary treatments (10 pens\/treatment; 30 birds\/pen): treatment 1 (NC), a non-medicated corn\u2013soybean basal diet; treatment 2 (PC), NC + 50 g\/metric ton (MT) of bacitracin methylene disalicylate (BMD); and treatments 3 (CQ15) and 4 (CQ30), NC + 15 and 30 g\/MT, respectively. On the day (d) of placement, birds were challenged by a coccidia vaccine to induce NE. On d 8, 14, 28, and 42, performance parameters were measured. On d 8, three birds\/pen were necropsied for NE lesions. On d 8 and d 14, jejunum samples from one bird\/pen were collected for mRNA abundance of tight junction proteins and nutrient transporter genes. Data were analyzed in JMP (JMP Pro, 16), and significance (p \u2264 0.05) between treatments was identified by Fisher\u2019s least significant difference (LSD) test. Compared to PC and NC, CQ15 had higher average daily gain (ADG), while CQ30 had lower average daily feed intake (ADFI) and feed conversion ratio (FCR). NE lesions in the duodenum were lower in CQ15 compared to all other treatments. On d 8, mRNA abundance of CLDN1, CLDN5, AMPK, PepT2, GLUT2, and EAAT3 were significantly greater in CQ30 (p &lt; 0.05) compared to both PC and NC. On d 14, mRNA abundance of ZO2 and PepT2 was significantly lower in PC when compared to all treatments, while that of ANXA1, JAM3, and GLUT5 was comparable to CQ15. In summary, adding Clarity Q to broiler diets has the potential to alleviate adverse effects caused by this enteric disease by improving performance, reducing intestinal lesions, and positively modulating the mRNA abundance of various tight junction proteins and key nutrient transporters during peak NE infection.<\/jats:p>","DOI":"10.3390\/microorganisms11081894","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T01:50:37Z","timestamp":1690422637000},"page":"1894","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inclusion of Quillaja Saponin Clarity Q Manages Growth Performance, Immune Response, and Nutrient Transport of Broilers during Subclinical Necrotic Enteritis"],"prefix":"10.3390","volume":"11","author":[{"given":"Candice E. C.","family":"Blue","sequence":"first","affiliation":[{"name":"Department of Poultry Science, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nima K.","family":"Emami","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallory B.","family":"White","sequence":"additional","affiliation":[{"name":"School of STEM, Virginia Western Community College, Roanoke, VA 24015, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Staci","family":"Cantley","sequence":"additional","affiliation":[{"name":"Huvepharma, Inc., Peachtree City, GA 30269, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rami A.","family":"Dalloul","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.anaerobe.2018.04.011","article-title":"Expansion of the Clostridium perfringens toxin-based typing scheme","volume":"53","author":"Rood","year":"2018","journal-title":"Anaerobe"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101330","DOI":"10.1016\/j.psj.2021.101330","article-title":"Centennial Review: Recent developments in host-pathogen interactions during necrotic enteritis in poultry","volume":"100","author":"Emami","year":"2021","journal-title":"Poult. 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