{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:49Z","timestamp":1787029009519,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union Reference Laboratory for Brucellosis","doi-asserted-by":"publisher","award":["SI2.801943"],"award-info":[{"award-number":["SI2.801943"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union Reference Laboratory for Brucellosis","doi-asserted-by":"publisher","award":["020602002001"],"award-info":[{"award-number":["020602002001"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union Reference Laboratory for Brucellosis","doi-asserted-by":"publisher","award":["2102054258"],"award-info":[{"award-number":["2102054258"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"French Directorate General for Food (DGAL)","award":["SI2.801943"],"award-info":[{"award-number":["SI2.801943"]}]},{"name":"French Directorate General for Food (DGAL)","award":["020602002001"],"award-info":[{"award-number":["020602002001"]}]},{"name":"French Directorate General for Food (DGAL)","award":["2102054258"],"award-info":[{"award-number":["2102054258"]}]},{"name":"Ministry for an Ecological and Solidary Transition","award":["SI2.801943"],"award-info":[{"award-number":["SI2.801943"]}]},{"name":"Ministry for an Ecological and Solidary Transition","award":["020602002001"],"award-info":[{"award-number":["020602002001"]}]},{"name":"Ministry for an Ecological and Solidary Transition","award":["2102054258"],"award-info":[{"award-number":["2102054258"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>France has been officially free of bovine brucellosis since 2005. Nevertheless, in 2012, as the source of two human cases, a bovine outbreak due to B. melitensis biovar 3 was confirmed in the French Alpine Bargy massif, due to a spillover from wild, protected Alpine ibex (Capra ibex). In order to reduce high Brucella prevalence in the local ibex population, successive management strategies have been implemented. Lateral flow immunochromatography assay (LFIA) was thus identified as a promising on-site screening test, allowing for a rapid diagnosis far from the laboratory. This study compared a commercial LFIA for brucellosis diagnosis with the WOAH-recommended tests for small ruminants (i.e., Rose Bengal test (RBT), Complement fixation test, (CFT) and Indirect ELISA, (iELISA)). LFIA showed the same analytical sensitivity as iELISA on successive dilutions of the International Standard anti-Brucella melitensis Serum (ISaBmS) and the EU Goat Brucella Standard Serum (EUGBSS). Selectivity was estimated at 100% when vaccinated ibex sera were analyzed. When used on samples from naturally infected ibex, LFIA showed high concordance, as well as relative sensitivity and specificity (&gt;97.25%) in comparison with RBT and CFT. This work shows high reliability and ensures a better standardization of LFIA testing for wild ruminants.<\/jats:p>","DOI":"10.3390\/microorganisms11081976","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:14:40Z","timestamp":1690881280000},"page":"1976","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of a Lateral Flow Immunochromatography Assay (LFIA) for Diagnosis and Surveillance of Brucellosis in French Alpine Ibex (Capra ibex)"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3395-4113","authenticated-orcid":false,"given":"Luca","family":"Freddi","sequence":"first","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3960-6646","authenticated-orcid":false,"given":"Acacia Ferreira","family":"Vicente","sequence":"additional","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Petit","sequence":"additional","affiliation":[{"name":"French Office for Biodiversity (OFB), Research and Scientific Support, 74320 S\u00e9vrier, France"},{"name":"The Biometrics and Evolutionary Biology Laboratory UMR 5558, CNRS, VetAgro Sup, Universit\u00e9 de Lyon, 69622 Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00ebline","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette","family":"Game","sequence":"additional","affiliation":[{"name":"Departmental Veterinary Laboratory of Savoie (LDAV 73), 73000 Chamb\u00e9ry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Locatelli","sequence":"additional","affiliation":[{"name":"R\u00e9serve Zoologique de la Haute Touche, Mus\u00e9um National d\u2019Histoire Naturelle (MNHN), 36290 Obterre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Jacques","sequence":"additional","affiliation":[{"name":"Institut Universitaire Technologique (IUT), D\u00e9partement G\u00e9nie Biologique, Universit\u00e9 de Tours, 37082 Tours, France"},{"name":"UMR-1282 Infectiologie et Sant\u00e9 Publique (ISP), INRAE Centre Val de Loire, Universit\u00e9 de Tours, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micka\u00ebl","family":"Riou","sequence":"additional","affiliation":[{"name":"UE-1277 Plateforme d\u2019Infectiologie Exp\u00e9rimentale, INRAE Centre Val de Loire, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryne","family":"Jay","sequence":"additional","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Garin-Bastuji","sequence":"additional","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Rossi","sequence":"additional","affiliation":[{"name":"French Office for Biodiversity (OFB), Research and Scientific Support, 74320 S\u00e9vrier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitomir","family":"Djokic","sequence":"additional","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9633-4464","authenticated-orcid":false,"given":"Claire","family":"Ponsart","sequence":"additional","affiliation":[{"name":"EU\/WOAH & National Reference Laboratory for Animal Brucellosis, ANSES\/Paris-Est University, 94700 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1016\/j.ajpath.2015.03.003","article-title":"Pathogenesis and immunobiology of brucellosis: Review of Brucella\u2013Host interactions","volume":"185","author":"Ficht","year":"2015","journal-title":"Am. J. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"Pappas","year":"2006","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1128\/microbiolspec.BAI-0006-2019","article-title":"The intracellular life cycle of Brucella spp.","volume":"7","author":"Celli","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"Franco","year":"2007","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1051\/vetres:2005003","article-title":"From the Discovery of the Malta fever\u2019s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis","volume":"36","author":"Godfroid","year":"2005","journal-title":"Vet. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"249","DOI":"10.20506\/rst.32.1.2197","article-title":"Economics of brucellosis impact and control in low-income countries","volume":"32","author":"McDermott","year":"2013","journal-title":"Rev. Sci. Tech. Int. Off. Epizoot."},{"key":"ref_7","unstructured":"Corbel, M.J., Food and Agriculture Organization of the United Nations, World Health Organization, and World Organisation for Animal Health (2006). Brucellosis in Humans and Animals, World Health Organization."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: A re-emerging zoonosis","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0167-5877(96)01110-5","article-title":"A Review of the use of B. melitensis Rev 1 vaccine in adult sheep and goats","volume":"31","author":"Blasco","year":"1997","journal-title":"Prev. Vet. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.20506\/rst.32.1.2180","article-title":"Brucellosis in terrestrial wildlife","volume":"32","author":"Godfroid","year":"2013","journal-title":"Rev. Sci. Tech. Int. Off. Epizoot."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority, and European Centre for Disease Prevention and Control (2018). The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-Borne Outbreaks in 2017. EFSA J., 16, e05500.","DOI":"10.2903\/j.efsa.2018.5500"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.3201\/eid2009.131517","article-title":"Reemergence of Brucella melitensis infection in wildlife, France","volume":"20","author":"Hars","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Mick, V., Le Carrou, G., Corde, Y., Game, Y., Jay, M., and Garin-Bastuji, B. (2014). Brucella melitensis in France: Persistence in wildlife and probable spillover from Alpine ibex to domestic animals. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094168"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20227","DOI":"10.2807\/ese.17.30.20227-en","article-title":"Re-emergence of brucellosis in cattle in France and risk for human health","volume":"17","author":"Mailles","year":"2012","journal-title":"Eurosurveillance"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"15592","DOI":"10.1038\/s41598-017-15803-w","article-title":"Sociospatial structure explains marked variation in brucellosis seroprevalence in an Alpine ibex population","volume":"7","author":"Marchand","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.3389\/fmicb.2018.01065","article-title":"High shedding potential and significant individual heterogeneity in naturally-infected Alpine ibex (Capra ibex) with Brucella melitensis","volume":"9","author":"Lambert","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"105239","DOI":"10.1016\/j.prevetmed.2020.105239","article-title":"Estimating disease prevalence and temporal dynamics using biased capture serological data in a wildlife reservoir: The example of brucellosis in Alpine ibex (Capra ibex)","volume":"187","author":"Calenge","year":"2021","journal-title":"Prev. Vet. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100542","DOI":"10.1016\/j.epidem.2022.100542","article-title":"Combining seroprevalence and capture-mark-recapture data to estimate the force of infection of brucellosis in a managed population of Alpine ibex","volume":"38","author":"Lambert","year":"2022","journal-title":"Epidemics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/s13567-019-0717-0","article-title":"Brucella melitensis Rev.1 vaccination generates a higher shedding risk of the vaccine strain in Alpine ibex (Capra ibex) compared to the domestic goat (Capra hircus)","volume":"50","author":"Ponsart","year":"2019","journal-title":"Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.vetimm.2016.02.002","article-title":"A review of the basis of the immunological diagnosis of ruminant brucellosis","volume":"171","author":"Ducrotoy","year":"2016","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3325\/cmj.2010.51.296","article-title":"Diagnosis of brucellosis in livestock and wildlife","volume":"51","author":"Godfroid","year":"2010","journal-title":"Croat. Med. J."},{"key":"ref_22","unstructured":"World Organisation for Animal Health (2022). OIE Terrestrial Manual 2022, World Organisation for Animal Health."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.vetmic.2008.01.009","article-title":"Simple and rapid field tests for brucellosis in livestock","volume":"130","author":"Abdoel","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_24","first-page":"423","article-title":"Comparative evaluation of standard serological tests for diagnosis of ovine brucellosis","volume":"2","author":"Ashraf","year":"2014","journal-title":"Benha Vet. Med. J."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Gusi, A.M., Bertu, W.J., de Miguel, M.J., Dieste-P\u00e9rez, L., Smits, H.L., Ocholi, R.A., Blasco, J.M., Moriy\u00f3n, I., and Mu\u00f1oz, P.M. (2019). Comparative performance of lateral flow immunochromatography, iELISA and rose bengal tests for the diagnosis of cattle, sheep, goat and swine brucellosis. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007509"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1080\/15321819.2018.1541803","article-title":"Protein-G-based lateral flow assay for rapid serodiagnosis of brucellosis in domesticated animals","volume":"40","author":"Manasa","year":"2019","journal-title":"J. Immunoass. Immunochem."},{"key":"ref_27","unstructured":"(2009). Animal Health Analysis Method\u2014Detection of Antibodies against Brucellosis by the Complement Fixation Test (Standard No. NF U47-004). Available online: https:\/\/www.boutique.afnor.org\/en-gb\/standard\/nf-u47004\/animal-health-analysis-methods-detection-of-antibodies-against-brucellosis-\/fa048555\/62829."},{"key":"ref_28","unstructured":"(2009). Animal Health Analysis Methods\u2014Detection of Antibodies against Brucellosis by the Rose-Bengal Plate Test (Standard No. NF U47-003). Available online: https:\/\/www.boutique.afnor.org\/en-gb\/standard\/nf-u47003\/animal-health-analysis-methods-detection-of-antibodies-against-brucellosis-\/fa159228\/32897."},{"key":"ref_29","first-page":"809","article-title":"The first international standard anti-Brucella melitensis serum","volume":"30","author":"McGiven","year":"2011","journal-title":"Rev. Sci. Tech. Int. Off. Epizoot."},{"key":"ref_30","unstructured":"(2015). Avis de l\u2019Anses et Rapport D\u2019expertise Collective, Maisons. Available online: https:\/\/www.anses.fr\/fr\/system\/files\/SANT2014sa0218Ra.pdf."},{"key":"ref_31","first-page":"97","article-title":"La R\u00e9introduction en France du bouquetin des Alpes","volume":"45","author":"Gauthier","year":"1990","journal-title":"Rev. Ecol."},{"key":"ref_32","unstructured":"(2019). Rapport D\u2019expertise Collective, Maisons. Available online: https:\/\/www.anses.fr\/fr\/system\/files\/SABA2018SA0017Ra.pdf."},{"key":"ref_33","unstructured":"RStudio Team (2020). RStudio: Integrated Development Environment for R 2020, RStudio, Inc."},{"key":"ref_34","unstructured":"R Core Team (2019). R: A Language and Environment for Statistical Computing Year, R Core Team."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/s13567-021-00984-0","article-title":"Targeted strategies for the management of wildlife diseases: The case of brucellosis in Alpine ibex","volume":"52","author":"Lambert","year":"2021","journal-title":"Vet. Res."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/8\/1976\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:23:34Z","timestamp":1760127814000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/8\/1976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,31]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["microorganisms11081976"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11081976","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,31]]}}}