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This facultative extracellular\u2013intracellular pathogen can be transmitted to humans, leading to a zoonotic disease. The disease remains a public health concern, particularly in regions where livestock farming is present. The two-component regulatory system BvrR\/BvrS was described by isolating the attenuated transposition mutants bvrR::Tn5 and bvrS::Tn5, whose characterization led to the understanding of the role of the system in bacterial survival. However, a phenotypic comparison with deletion mutants has not been performed because their construction has been unsuccessful in brucellae and difficult in phylogenetically related Rhizobiales with BvrR\/BvrS orthologs. Here, we used an unmarked gene excision strategy to generate a B. abortus mutant strain lacking both genes, called B. abortus\u00a0\u2206bvrRS. The deletion was verified through PCR, Southern blot, Western blot, Sanger sequencing, and whole-genome sequencing, confirming a clean mutation without further alterations at the genome level. B. abortus\u00a0\u2206bvrRS shared attenuated phenotypic traits with both transposition mutants, confirming the role of BvrR\/BvrS in pathogenesis and membrane integrity. This B. abortus\u00a0\u2206bvrRS with a non-antimicrobial marker is an excellent tool for continuing studies on the role of BvrR\/BvrS in the B. abortus lifestyle.<\/jats:p>","DOI":"10.3390\/microorganisms11082014","type":"journal-article","created":{"date-parts":[[2023,8,5]],"date-time":"2023-08-05T10:21:39Z","timestamp":1691230899000},"page":"2014","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A bvrR\/bvrS Non-Polar Brucella abortus Mutant Confirms the Role of the Two-Component System BvrR\/BvrS in Virulence and Membrane Integrity"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0990-149X","authenticated-orcid":false,"given":"Olga","family":"Rivas-Solano","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Instituto Tecnol\u00f3gico de Costa Rica, Cartago 30109, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8629-5107","authenticated-orcid":false,"given":"Kattia","family":"N\u00fa\u00f1ez-Montero","sequence":"additional","affiliation":[{"name":"Laboratorio Facultad Ciencias de la Salud, Instituto de Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Chile, Temuco 4813003, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Altamirano-Silva","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Enfermedades Tropicales, Universidad de Costa Rica, San Pedro de Montes de Oca, San Jos\u00e9 2060, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7746-6920","authenticated-orcid":false,"given":"Nazareth","family":"Ruiz-Villalobos","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Universidad Nacional, Heredia 40104, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-3340","authenticated-orcid":false,"given":"El\u00edas","family":"Barquero-Calvo","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Universidad Nacional, Heredia 40104, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Universidad Nacional, Heredia 40104, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Enfermedades Tropicales, Universidad de Costa Rica, San Pedro de Montes de Oca, San Jos\u00e9 2060, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1036-920X","authenticated-orcid":false,"given":"Caterina","family":"Guzm\u00e1n-Verri","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Universidad Nacional, Heredia 40104, Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,5]]},"reference":[{"key":"ref_1","unstructured":"Corbel, M.J., Food and Agriculture Organization of the United Nations, World Health Organization, and World Organisation for Animal Health (2006). Brucellosis in Humans and Animals."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0378-1135(02)00214-6","article-title":"Brucella Intracellular Life: From Invasion to Intracellular Replication","volume":"90","author":"Gorvel","year":"2002","journal-title":"Vet. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1073\/pnas.022622699","article-title":"Brucella melitensis: A Nasty Bug with Hidden Credentials for Virulence","volume":"99","author":"Moreno","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1046\/j.1365-2958.1998.00913.x","article-title":"A Two-component Regulatory System Playing a Critical Role in Plant Pathogens and Endosymbionts Is Present in Brucella abortus and Controls Cell Invasion and Virulence","volume":"29","author":"Moreno","year":"1998","journal-title":"Mol. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00713-17","DOI":"10.1128\/IAI.00713-17","article-title":"Brucella abortus Senses the Intracellular Environment through the BvrR\/BvrS Two-Component System, Which Allows B. Abortus To Adapt to Its Replicative Niche","volume":"86","author":"Moreno","year":"2018","journal-title":"Infect. Immun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e00004-21","DOI":"10.1128\/IAI.00004-21","article-title":"Intracellular Passage Triggers a Molecular Response in Brucella abortus That Increases Its Infectiousness","volume":"89","author":"Moreno","year":"2021","journal-title":"Infect. Immun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"12375","DOI":"10.1073\/pnas.192439399","article-title":"The Two-Component System BvrR\/BvrS Essential for Brucella abortus Virulence Regulates the Expression of Outer Membrane Proteins with Counterparts in Members of the Rhizobiaceae","volume":"99","author":"Manterola","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41564-020-00799-3","article-title":"\u03b2-Barrels Covalently Link Peptidoglycan and the Outer Membrane in the \u03b1-Proteobacterium Brucella abortus","volume":"6","author":"Godessart","year":"2021","journal-title":"Nat. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e13164","DOI":"10.1111\/cmi.13164","article-title":"Omp25-dependent Engagement of SLAMF1 by Brucella abortus in Dendritic Cells Limits Acute Inflammation and Favours Bacterial Persistence in Vivo","volume":"22","author":"Degos","year":"2020","journal-title":"Cell. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Viadas, C., Rodr\u00edguez, M.C., Sangari, F.J., Gorvel, J.-P., Garc\u00eda-Lobo, J.M., and L\u00f3pez-Go\u00f1i, I. (2010). Transcriptome Analysis of the Brucella abortus BvrR\/BvrS Two-Component Regulatory System. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010216"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rivas-Solano, O., Van der Henst, M., Castillo-Zeled\u00f3n, A., Su\u00e1rez-Esquivel, M., Mu\u00f1oz-Vargas, L., Capitan-Barrios, Z., Thomson, N.R., Chaves-Olarte, E., Moreno, E., and De Bolle, X. (2022). The Regulon of Brucella abortus Two-Component System BvrR\/BvrS Reveals the Coordination of Metabolic Pathways Required for Intracellular Life. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0274397"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2929","DOI":"10.1016\/j.febslet.2011.08.011","article-title":"Brucella Adaptation and Survival at the Crossroad of Metabolism and Virulence","volume":"585","author":"Barbier","year":"2011","journal-title":"FEBS Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1128\/jb.170.9.4249-4256.1988","article-title":"Rhizobium Meliloti Mutants That Overproduce the R. meliloti Acidic Calcofluor-Binding Exopolysaccharide","volume":"170","author":"Doherty","year":"1988","journal-title":"J. Bacteriol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5485","DOI":"10.1128\/jb.177.19.5485-5494.1995","article-title":"Identification of Rhizobium-Specific Intergenic Mosaic Elements within an Essential Two-Component Regulatory System of Rhizobium Species","volume":"177","author":"Stanley","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/JB.180.1.20-26.1998","article-title":"Succinoglycan Production by Rhizobium meliloti Is Regulated through the ExoS-ChvI Two-Component Regulatory System","volume":"180","author":"Cheng","year":"1998","journal-title":"J. Bacteriol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1365-2958.2007.05680.x","article-title":"ExoR Is Genetically Coupled to the ExoS?ChvI Two-Component System and Located in the Periplasm of Sinorhizobium meliloti","volume":"64","author":"Wells","year":"2007","journal-title":"Mol. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1111\/j.1365-2958.2009.06931.x","article-title":"Null Mutations in Sinorhizobium meliloti ExoS and ChvI Demonstrate the Importance of This Two-Component Regulatory System for Symbiosis","volume":"74","author":"Dimmick","year":"2009","journal-title":"Mol. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6614","DOI":"10.1128\/jb.175.20.6614-6625.1993","article-title":"A Chromosomally Encoded Two-Component Sensory Transduction System Is Required for Virulence of Agrobacterium tumefaciens","volume":"175","author":"Charles","year":"1993","journal-title":"J. Bacteriol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6626","DOI":"10.1128\/jb.175.20.6626-6636.1993","article-title":"The Chromosomal Response Regulatory Gene ChvI of Agrobacterium tumefaciens Complements an Escherichia coli PhoB Mutation and Is Required for Virulence","volume":"175","author":"Mantis","year":"1993","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wu, C.-F., Lin, J.-S., Shaw, G.-C., and Lai, E.-M. (2012). Acid-Induced Type VI Secretion System Is Regulated by ExoR-ChvG\/ChvI Signaling Cascade in Agrobacterium tumefaciens. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002938"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3352","DOI":"10.1128\/JB.01676-09","article-title":"The BatR\/BatS Two-Component Regulatory System Controls the Adaptive Response of Bartonella henselae during Human Endothelial Cell Infection","volume":"192","author":"Quebatte","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.tube.2015.08.009","article-title":"Comparison of Transposon and Deletion Mutants in Mycobacterium tuberculosis: The Case of Rv1248c, Encoding 2-Hydroxy-3-Oxoadipate Synthase","volume":"95","author":"Maksymiuk","year":"2015","journal-title":"Tuberculosis"},{"key":"ref_23","first-page":"1557","article-title":"Brucella abortus Strain 2308 Wisconsin Genome: Importance of the Definition of Reference Strains","volume":"7","author":"Comerci","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1128\/JB.05623-11","article-title":"The RNA Chaperone Hfq Independently Coordinates Expression of the VirB Type IV Secretion System and the LuxR-Type Regulator BabR in Brucella abortus 2308","volume":"194","author":"Caswell","year":"2012","journal-title":"J. Bacteriol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1365-2958.2012.08117.x","article-title":"Identification of Two Small Regulatory RNAs Linked to Virulence in Brucella abortus 2308: Brucella Small RNAs Are Required for Virulence","volume":"85","author":"Caswell","year":"2012","journal-title":"Mol. Microbiol."},{"key":"ref_26","unstructured":"Alton, G.G., Jones, L.M., Angus, R.D., and Verger, J.M. (1988). Techniques for the Brucellosis Laboratory."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Castillo-Zeled\u00f3n, A., Ruiz-Villalobos, N., Altamirano-Silva, P., Chac\u00f3n-D\u00edaz, C., Barquero-Calvo, E., Chaves-Olarte, E., and Guzm\u00e1n-Verri, C. (2021). A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR Ortholog Is Not Crucial for Brucella abortus Virulence. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0254568"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschu","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1093\/bioinformatics\/bty149","article-title":"NanoPack: Visualizing and Processing Long-Read Sequencing Data","volume":"34","author":"Schultz","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving Bacterial Genome Assemblies from Short and Long Sequencing Reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: Quality Assessment Tool for Genome Assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: Assessing the Quality of Microbial Genomes Recovered from Isolates, Single Cells, and Metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"ref_33","unstructured":"(2022, October 24). BLAST Help Page, Available online: https:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi?CMD=Web&amp;PAGE_TYPE=BlastDocs&amp."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"giab063","DOI":"10.1093\/gigascience\/giab063","article-title":"Vulcan: Improved Long-Read Mapping and Structural Variant Calling via Dual-Mode Alignment","volume":"10","author":"Fu","year":"2021","journal-title":"GigaScience"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3422","DOI":"10.1093\/bioinformatics\/bti553","article-title":"ACT: The Artemis Comparison Tool","volume":"21","author":"Carver","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e00312-18","DOI":"10.1128\/IAI.00312-18","article-title":"Transposon Sequencing of Brucella abortus Uncovers Essential Genes for Growth In Vitro and Inside Macrophages","volume":"86","author":"Sternon","year":"2018","journal-title":"Infect. Immun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5631","DOI":"10.1128\/JB.187.16.5631-5639.2005","article-title":"The Lipopolysaccharide of Brucella abortus BvrS\/BvrR Mutants Contains Lipid A Modifications and Has Higher Affinity for Bactericidal Cationic Peptides","volume":"187","author":"Manterola","year":"2005","journal-title":"J. Bacteriol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/8\/2014\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:26:20Z","timestamp":1760127980000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/8\/2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,5]]},"references-count":37,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["microorganisms11082014"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11082014","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,5]]}}}