{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:12:17Z","timestamp":1788984737195,"version":"build-2803163510"},"reference-count":87,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cholera, a disease caused by the Vibrio cholerae bacteria, threatens public health worldwide. The organism mentioned above has a significant historical record of being identified as a prominent aquatic environmental pollutant capable of adapting its phenotypic and genotypic traits to react to host patients effectively. This study aims to elucidate the heterogeneity of the sporadic clinical strain of V. cholerae VC01 among patients residing in Silvasa. The study involved conducting whole-genome sequencing of the isolate obtained from patients exhibiting symptoms, including those not commonly observed in clinical practice. The strain was initially identified through a combination of biochemical analysis, microscopy, and 16s rRNA-based identification, followed by type strain-based identification. The investigation demonstrated the existence of various genetic alterations and resistance profiles against multiple drugs, particularly chloramphenicol (catB9), florfenicol (floR), oxytetracycline (tet(34)), sulfonamide (sul2), and Trimethoprim (dfrA1). The pan-genomic analysis indicated that 1099 distinct clusters were detected within the genome sequences of recent isolates worldwide. The present study helps to establish a correlation between the mutation and the coexistence of antimicrobial resistance toward current treatment.<\/jats:p>","DOI":"10.3390\/microorganisms11082030","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T12:35:42Z","timestamp":1691498142000},"page":"2030","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-Resistant Vibrio cholerae VC01 Isolated from a Clinical Sample"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0739-026X","authenticated-orcid":false,"given":"Vishal","family":"Mevada","sequence":"first","affiliation":[{"name":"DNA Division, Directorate of Forensic Science, Gandhinagar 382007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Veer Narmad South Gujarat University, Surat 395007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4041-6751","authenticated-orcid":false,"given":"Pravin","family":"Dudhagara","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Veer Narmad South Gujarat University, Surat 395007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Chaudhari","sequence":"additional","affiliation":[{"name":"School of Applied Sciences and Technology, Gujarat Technological University, Ahmedabad 382424, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5473-5219","authenticated-orcid":false,"given":"Mustafa","family":"Vohra","sequence":"additional","affiliation":[{"name":"Directorate of Medical & Health Services, UT of Dadra & Nagar Haveli and Daman & Diu, Silvassa 396230, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram","family":"Khan","sequence":"additional","affiliation":[{"name":"Directorate of Medical & Health Services, UT of Dadra & Nagar Haveli and Daman & Diu, Silvassa 396230, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9325-4920","authenticated-orcid":false,"given":"Douglas","family":"J. H. Shyu","sequence":"additional","affiliation":[{"name":"Department of Biological Science and Technology, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yih-Yuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, National Chiayi University, Chiayi City 600, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dolatsinh","family":"Zala","sequence":"additional","affiliation":[{"name":"School of Applied Sciences and Technology, Gujarat Technological University, Ahmedabad 382424, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ali, M., Nelson, A.R., Lopez, A.L., and Sack, D.A. (2015). Updated Global Burden of Cholera in Endemic Countries. PLoS Negl. Trop. 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